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BR-102024017779-A2 - Polymeric films based on chitosan and sage oil-resin (Rosmarinus spenne): production process, compositions and uses.

BR102024017779A2BR 102024017779 A2BR102024017779 A2BR 102024017779A2BR-102024017779-A2

Abstract

This invention relates to a process for obtaining polymeric films based on chitosan and rosemary oil-resin (Salvia rosmarinus SPENN L.), their compositions and uses. The technology enhances the antimicrobial potential of its constituents through the synergism that occurs between chitosan and oil-resin, aiming at the management and treatment of secondary infections associated with skin lesions. This invention addresses the process of obtaining chitosan films incorporated with rosemary oil-resin (Salvia rosmarinus SPENN L.), providing as a product a malleable, biodegradable film with antimicrobial action. Rosemary oil-resin is known to have great antimicrobial potential. Chitosan, in turn, is an excellent ingredient for various formulation models, including adhesive films. In these films, chitosan provides a controlled release system for the active components of the extract, in addition to enhancing the antimicrobial effects. By incorporating these two active ingredients, chitosan and oleoresin, into the adhesive films, the effect of the oleoresin becomes even more efficient and can be used topically for secondary infections associated with skin lesions.

Inventors

  • ANA CAROLINA MORAIS APOLÔNIO
  • RODRIGO LUIZ FABRI
  • ANA BÁRBARA POLO
  • GUILHERME DINIZ TAVARES
  • TALEESSA VIEIRA GOMES
  • MARIANA SIMÕES DE OLIVEIRA
  • MILLENA MACHADO CARDOSO
  • BEATRIZ VILELA TOMÉ
  • ARI SÉRGIO DE OLIVEIRA LEMOS

Assignees

  • UNIVERSIDADE FEDERAL DE JUIZ DE FORA - UFJF

Dates

Publication Date
20260310
Application Date
20240829

Claims (10)

  1. 1. POLYMERIC FILMS BASED ON CHITOSAN AND SALVIA ROSMARINUS SPENN OLEO-RESIN, characterized by comprising: a) Purified water (60.41 mL); b) Malic acid (1.4 g); c) Chitosan (1.4 g); d) Glycerin (7 mL); e) Tween 80 (340 μL in each beaker); f) Salvia rosmarinus Spenn oleo-resin (0.085 g; 0.034 g; 0.17 g).
  2. 2. PROCESS FOR OBTAINING POLYMERIC FILMS BASED ON CHITOSAN AND SALVIA ROSMARINUS SPENN OLEORESIN, according to claim 1, characterized by comprising the steps of: a) Weighing and reserving the components; b) Addition of malic acid and purified water to a beaker, followed by the addition of chitosan, which is then placed on a mechanical stirrer (1200 rpm) for 1 h until complete dissolution of the polymer; c) Addition of glycerin, maintaining agitation for another 30 min; d) Division of the base into 4 duly identified beakers for subsequent addition of different concentrations of Salvia rosmarinus Spenn oleoresin emulsified in Tween 80 in its respective beaker, which will be placed on a magnetic stirrer until complete dissolution; e) Degassing of the samples in an ultrasonic bath for 2 h; f) Each beaker is poured into a petri dish, which is then dried in an oven at 50 °C for 12 hours.
  3. 3. PROCESS FOR OBTAINING POLYMERIC FILMS BASED ON CHITOSAN AND SALVIA ROSMARINUS SPENN OLEO-RESIN, according to claim 2, characterized in that the chitosan is weighed and suspended in a 1% (w/v) aqueous solution of malic acid in a beaker, which will be subjected to mechanical stirring (1200 rpm) for 1 h.
  4. 4. PROCESS FOR OBTAINING POLYMERIC FILMS BASED ON CHITOSAN AND SALVIA ROSMARINUS SPENN OLEO-RESIN, according to claim 1-3, characterized by the polymeric films exhibiting antimicrobial activity superior to their isolated compounds.
  5. 5. USE OF POLYMERIC FILMS BASED ON CHITOSAN AND SALVIA ROSMARINUS SPENN OLEORESIN, according to claim 1-4, characterized by being an antimicrobial pharmaceutical formulation.
  6. 6. Formulation of polymeric films based on chitosan and Salvia rosmarinus Spenn oleoresin, according to claims 4 and 5, characterized by the synergism between chitosan and Salvia rosmarinus Spenn oleoresin.
  7. 7. USE OF A POLYMERIC FILM FORMULATION BASED ON CHITOSAN AND SALVIA ROSMARINUS SPENN OLEO-RESIN, according to claim 1-6, characterized by exhibiting antimicrobial potential against secondary infections associated with topical lesions.
  8. 8. USE OF A POLYMERIC FILM FORMULATION BASED ON CHITOSAN AND SALVIA ROSMARINUS SPENN OLEO-RESIN, according to claim 1-7, characterized by exhibiting antimicrobial potential against secondary infections associated with burns.
  9. 9. USE OF A POLYMERIC FILM FORMULATION BASED ON CHITOSAN AND SALVIA ROSMARINUS SPENN OLEO-RESIN, according to claims 1-8, characterized by exhibiting antimicrobial potential against secondary infections associated with diabetic foot.
  10. 10. USE OF A POLYMERIC FILM FORMULATION BASED ON CHITOSAN AND SALVIA ROSMARINUS SPENN OLEO-RESIN, according to claim 1-9, characterized by exhibiting antimicrobial potential against secondary infections associated with pressure ulcers.

Description

FIELD OF APPLICATION [001] The invention described in this document relates to the process of obtaining polymeric films based on chitosan and rosemary oil-resin (Salvia rosmarinus Spenn), their composition, characterization and uses. The combination of chitosan polymer and rosemary oil-resin in polymeric films exerts a synergistic effect in relation to their antimicrobial potential, aiming at the control of secondary infections in skin lesions. STATE OF THE ART [002] Formulations that combine natural compounds are frequently studied and developed, gaining strength in the current scenario during the fight against resistant microorganisms. Salvia rosmarinus Spenn belongs to the Lamiaceae family and originated in the Mediterranean, being widely cultivated and distributed throughout the world today (BEGUM, A. et al. An in-depth review on the medicinal flora Rosmarinus officinalis (Lamiaceae). Acta Scientiarum Polonorum, Technologia Alimentaria, v. 12, n. 1, p. 61-73, 2013). However, its chemical composition varies according to the plant varieties and growing conditions (PINTORE, G.A.M. et al. Chemical composition and antimicrobial activity of Salvia rosmarinus Spenn oils from Sardinia and Corsica. Flavor and Fragrance Journal, v. 17, p. 15-19, 2002), presenting interesting biological activities in the management of secondary infections to skin lesions. [003] The biological properties of rosemary have been recognized since antiquity, and the groups of active chemical constituents found in the plant have been gradually identified, considering their complex nature. Although there is great variation in the chemical composition of oils extracted from rosemary, there is a consensus in the literature that monoterpenes are the major compounds in essential oils, sometimes being hydrocarbons such as pinene, myrcene and camphene, and sometimes being oxygenated compounds such as camphor, 1,8-cineole and borneol (PORTE, A. GODOY, R. L. de O. Rosemary (Salvia rosmarinus Spenn): antimicrobial and chemical properties of the essential oil. Bulletin of the Food Research and Processing Center, Curitiba, v. 19, n. 2, p.193-210, Jul./Dec. 2001), while in oleoresins most of the compounds are diterpenic acids, such as carnosic acid, carnosol and rosmanol derivatives (Islamcevic, R.M. et al. Determination of Major Phenolic Acids, Phenolic Diterpenes and Triterpenes in Rosemary (Salvia rosmarinus Spenn) by Gas Chromatography and Mass Spectrometry. Acta Chimica Slovenica, vol. 54, no. 1, p. 60, 2007). [004] The antimicrobial activity of rosemary cannot be attributed to a specific chemical component, but rather to the synergistic effect of various substances, which also include rosmarinic acid, an important phenolic component against microorganisms. Studies indicate that the components responsible for the antimicrobial effect of the plant are those containing aromatic nuclei and polar functional groups, with hydroxylated and carbonyl compounds being the main ones (PORTE, A. GODOY, R. L. de O. Rosemary (Salvia rosmarinus Spenn): antimicrobial properties and chemistry of the essential oil. Bulletin of the Food Research and Processing Center, Curitiba, v. 19, n. 2, p.193-210, Jul./Dec. 2001). [005] Furthermore, authors point out that Gram-positive bacteria are generally more sensitive to condiment plants than Gram-negative bacteria. This is probably due to the fact that the lipopolysaccharide layer contained in the cell wall of Gram-negative bacteria prevents the plant components from reaching the microorganism's cytoplasmic membrane and exerting their effect (VAARA, M. Agents that increase the permeability of the outer membrane. Microbiology Reviews, v. 56, n. 3, p. 395-411, 1992.) [006] The correct use of topical medications is important, as misuse can lead to therapy failure and drug resistance (PATEL, R. et al. Burn assessment: A critical review on care, advances in burn healing and pre-clinical animal studies. Journal of Research in Pharmacy, v. 27, n. 4, p. 1577-1593, 2023). Polymeric films can be especially useful for the treatment of skin infections secondary to skin lesions. They have unique characteristics that make them functional films and can act in the prevention and treatment of infections, such as moisture control in the wound, removal of excess exudate, formation of a semipermeable barrier, and the presence of compounds that have an antimicrobial effect (AGARWAL, A. et al. Polymeric materials for chronic wound and burn dressings. Advanced Wound Repair Therapies, Woodhead Publishing, p. 186-208, 2011). [007] Some claims are already found with this motivation, such as patents BR 10 2022 005720 6, BR 11 2023 006623 3 A2, BR 10 2020 0201050 5 and C1 0803807-4 F1, in which the main objectives were to describe the process of obtaining polymeric films as carriers of bioactive substances, as in the case of patent BR 10 2016 017854 1, in which polymeric films with copaiba oil were developed for therapeutic application. [008] Polymer