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BR-112018073180-B1 - METHOD FOR SELECTING A SORGHUM PLANT THAT EXHIBITS GREATER RESISTANCE TO ANTHRACNOSE

BR112018073180B1BR 112018073180 B1BR112018073180 B1BR 112018073180B1BR-112018073180-B1

Abstract

Compositions and methods useful for identifying and selecting sorghum plants with increased resistance to anthracnose are provided in this document. The methods use molecular genetic markers within QTLs located on chromosome 5 and chromosome 8 to identify and select plants with increased resistance to anthracnose, and plants comprising the QTL alleles associated with increased anthracnose resistance can be crossed with other sorghum plants to incorporate the increased resistance into other sorghum lines or varieties.

Inventors

  • MALLESWARI GELLI
  • DAVID ALAN HESSEL

Assignees

  • PIONEER HI-BRED INTERNATIONAL, INC

Dates

Publication Date
20260317
Application Date
20170502
Priority Date
20160510

Claims (8)

  1. 1. Method for identifying and/or selecting a sorghum plant that has greater resistance to anthracnose, characterized in that it comprises: a. screening a population with a marker to determine whether one or more sorghum plants in the population comprise a favorable QTL allele associated with greater resistance to anthracnose, wherein the marker is located within a range on chromosome 5 comprising and flanked by V01153-1, having the reference sequence as established by SEQ ID NO: 3 and V01162-1, having the reference sequence as established by SEQ ID NO: 1; and b. Select from the aforementioned population at least one sorghum plant comprising the favorable allele, wherein the aforementioned QTL allele associated with greater resistance to anthracnose comprises at least one of the following: a “T” in V100EF5-1 at position 39 of SEQ ID NO:7, a “C” in V02017-1 at position 25 of SEQ ID NO:6, and a “G” in V02249-1 at position 25 of SEQ ID NO:2.
  2. 2. Method, according to claim 1, characterized in that said QTL allele associated with greater resistance to anthracnose comprises: a “T” in V100EF5-1 at position 39 of SEQ ID NO: 7, a “C” in V02017-1 at position 25 of SEQ ID NO: 6 and a “G” in V02249-1 at position 25 of SEQ ID NO: 2.
  3. 3. Method for identifying and/or selecting a sorghum plant that has greater resistance to anthracnose, characterized in that it comprises: a. screening a population with a marker to determine whether one or more sorghum plants in the population comprise a QTL allele associated with greater resistance to anthracnose, wherein the marker is located within a range on chromosome 8 comprising and flanked by V02395-1, having the reference sequence as established by SEQ ID NO: 4, and V02069-1, having the reference sequence as established by SEQ ID NO: 8; and b. Select from the aforementioned population at least one sorghum plant comprising the favorable allele, wherein the aforementioned QTL allele associated with greater resistance to anthracnose comprises at least one of the following: a “G” in V02068-1 at position 24 of SEQ ID NO:5 and a “G” in V02069-1 at position 29 of SEQ ID NO:8.
  4. 4. Method, according to claim 3, characterized in that the said QTL allele associated with greater resistance to anthracnose comprises: a “G” in V02068-1 at position 24 of SEQ ID NO:5 and a “G” in V02069-1 at position 29 of SEQ ID NO:8.
  5. 5. Method for identifying and/or selecting a sorghum plant that exhibits greater resistance to anthracnose, characterized in that it comprises: a. detecting in a sorghum plant an allele of a marker locus, wherein said marker locus is located within a chromosomal interval on chromosome 5 comprising and flanked by V01153-1, having the reference sequence as established by SEQ ID NO: 3 and V01162-1, having the reference sequence as established by SEQ ID NO: 1 and said allele is associated with a haplotype comprising: a “T” in V100EF5-1 at position 39 of SEQ ID NO: 7, a “C” in V02017-1 at position 25 of SEQ ID NO: 6, and a “G” in V02249-1 at position 25 of SEQ ID NO: 2; and b. Select a sorghum plant that has the allele of the marker locus that is associated with the haplotype.
  6. 6. Method for identifying and/or selecting a sorghum plant that exhibits greater resistance to anthracnose, characterized in that it comprises: a. detecting in a sorghum plant one or more of the following: i. a “T” in V100EF5-1 at position 39 of SEQ ID NO:7, ii. a “C” in V02017-1 at position 25 of SEQ ID NO:6, and iii. a “G” in V02249-1 at position 25 of SEQ ID NO:2; and b. selecting said sorghum plant that has one or more of (i) to (iii).
  7. 7. Method for identifying and/or selecting a sorghum plant that exhibits greater resistance to anthracnose, characterized in that it comprises: a. detecting in a sorghum plant an allele of a marker locus, wherein said marker locus is located within a chromosomal interval on chromosome 8 comprising and flanked by V02395-1, having the reference sequence as established by SEQ ID NO: 4, and V02069-1, having the reference sequence as established by SEQ ID NO: 8, and said allele is associated with a haplotype comprising: a “G” in V02068-1 at position 24 of SEQ ID NO: 5 and a “G” in V02069-1 at position 29 of SEQ ID NO: 8; and b. selecting a sorghum plant that has the allele of the marker locus that is associated with the haplotype.
  8. 8. Method for identifying and/or selecting a sorghum plant that exhibits greater resistance to anthracnose, characterized in that it comprises: a. detecting in a sorghum plant one or more of the following: i. a “G” in V02068-1 at position 24 of SEQ ID NO:5 and ii. a “G” in V02069-1 at position 29 of SEQ ID NO:8; and b. selecting said sorghum plant that has one or more of (a)(i) and (a)(ii).

Description

FIELD [0001] The field is located in the plant breeding and genetics area. REFERENCE TO THE LIST OF SEQUENCES SUBMITTED ELECTRONICALLY [0002] The official copy of the sequence listing is submitted electronically via EFS-Web as an ASCII-format sequence listing in a file named BB2504WOPCT_SequenceListing_ST25.txt, created on March 23, 2017, with a size of 2 kilobytes, and is deposited simultaneously with the descriptive report. The sequence listing contained in this document in ASCII format is part of the descriptive report and is incorporated herein in its entirety by reference. BACKGROUND [0003] Sorghum is the fifth most important cereal crop in the world, after wheat, rice, corn, and barley. In addition to being an increasingly important food crop for the world's population, sorghum is also an important animal feed used in many countries. Furthermore, sorghum is currently the second largest source of grain-based ethanol in the U.S. after corn. [0004] Anthracnose (Colletotrichum sublineolum) is an important fungal pathogen of grain sorghum that can lead to significant yield losses (20 to 80%) in susceptible genotypes, particularly in tropical and subtropical regions. All parts of the plants are affected. Crop rotation is suggested for controlling anthracnose infection; however, it is not always feasible and, more importantly, not always effective. Host plant resistance is the most effective form of control. [0005] Selection through the use of molecular markers associated with greater resistance to anthracnose allows selections based solely on the genetic makeup of the offspring. As a result, plant breeding can occur more rapidly, thus generating sorghum plants with greater resistance to anthracnose. SUMMARY [0006] Compositions and methods for identifying and/or selecting (i.e., obtaining) sorghum plants that have greater resistance to anthracnose are provided in this document. [0007] In one embodiment, a method for identifying and/or selecting a sorghum plant with increased resistance to anthracnose is presented in this document, wherein the method comprises the steps of: (a) screening a population with a marker to determine whether one or more sorghum plants in the population comprise a QTL allele associated with increased resistance to anthracnose, wherein the marker is located within a gap on chromosome 5 comprising and flanked by V01153-1 and V01162-1; and (b) selecting from said population at least one sorghum plant comprising the favorable allele. The method may further comprise: (c) crossing the sorghum plant with a second sorghum plant; and (d) obtaining a progeny plant that has the favorable allele. The method may include selecting a sorghum plant from a breeding program if the QTL allele is detected, or counter-selecting a sorghum plant if the QTL allele is not detected. In one aspect, the QTL allele associated with greater anthracnose resistance comprises at least one of the following: a “T” in V100EF5-1, a “C” in V02017-1, and a “G” in V02249-1. In another aspect, the QTL allele associated with greater anthracnose resistance comprises: a “T” in V100EF5-1, a “C” in V02017-1, and a “G” in V02249-1. [0008] In another embodiment, a method for identifying and/or selecting a sorghum plant with increased resistance to anthracnose is presented in this document, wherein the method comprises the steps of: (a) screening a population with a marker to determine whether one or more sorghum plants in the population comprise a QTL allele associated with increased resistance to anthracnose, wherein the marker is located within a gap on chromosome 8 comprising and flanked by V02395-1 and V02069-1; and (b) selecting from said population at least one sorghum plant comprising the favorable allele. The method may further comprise: (c) crossing the sorghum plant with a second sorghum plant; and (d) obtaining a progeny plant that has the favorable allele. The method may include selecting a sorghum plant from a breeding program if the QTL allele is detected, or counter-selecting a sorghum plant if the QTL allele is not detected. In one aspect, the QTL allele associated with increased anthracnose resistance comprises at least one of the following: a “G” in V02068-1 and a “G” in V02069-1. In another aspect, the QTL allele associated with increased anthracnose resistance comprises: a “G” in V02068-1 and a “G” in V02069-1. [0009] In another embodiment, a method for identifying and/or selecting a sorghum plant that exhibits greater resistance to anthracnose is provided in this document. The method comprises the steps of (a) detecting in a sorghum plant an allele of a marker locus, wherein said marker locus is located within a chromosomal gap on chromosome 5 comprising and flanked by V01153-1 and V01162-1, and said allele is associated with a haplotype comprising: a “T” in V100EF5-1, a “C” in V02017-1 and a “G” in V02249-1; (b) selecting a sorghum plant that has the allele of the marker locus that is associated with the haplo