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CA-3019167-C - ENCLOSED DROPLET SORTER AND METHODS OF USING THE SAME

CA3019167CCA 3019167 CCA3019167 CCA 3019167CCA-3019167-C

Abstract

Aspects of the present disclosure include a particle sorting module for sorting components of a sample, such as cells in a biological sample. Particle sorting modules according to certain embodiments include an enclosed housing having an aligner for coupling the housing with a particle sorting system, a flow cell nozzle positioned at the proximal end of the housing, a sample interrogation region in fluid communication with the orifice of the flow cell nozzle and a droplet deflector. A particle sorting system and methods for separating components of a sample as well as kits, including one or more particle sorting module, suitable for coupling with a particle sorting system and for practicing the subject methods are also provided.

Inventors

  • Pierce O. Norton

Assignees

  • BECTON, DICKINSON AND COMPANY

Dates

Publication Date
20260505
Application Date
20170328
Priority Date
20160415

Claims (13)

  1. 62 What is claimed is: 1. A particle sorting module comprising: a housing comprising a proximal end and a distal end and a wall therebetween, the wall comprising an aligner for aligning the housing with a particle sorting system; a flow cell nozzle positioned at the proximal end of the housing, the flow cell 5 nozzle comprising an orifice; a sample interrogation region in fluid communication with the flow cell nozzle orifice; and a droplet deflector positioned distal to the sample interrogation region, wherein the housing encloses the flow cell nozzle, the sample interrogation 10 region, and the droplet deflector in a sealed environment.
  2. 2. The particle sorting module according to claim 1, wherein the aligner comprises protrusions. 15
  3. 3. The particle sorting module according to claim 1 or 2, further comprising two or more sample collection containers.
  4. 4. The particle sorting module according to claim 3, wherein the sample collection containers are fastened to the distal end of the housing.
  5. 5. The particle sorting module according to any one of claims 1 to 4, further comprising a cuvette positioned in the sample interrogation region.
  6. 6. The particle sorting module according to any one of claims 1 to 5, further 25 comprising a sample inlet at the proximal end of the housing.
  7. 7. The particle sorting module according to any one of claims 1 to 6, further comprising a sheath fluid inlet at the proximal end of the housing. 30
  8. 8. The particle sorting module according to any one of claims 1 to 7, further comprising one or more electrical connectors on the housing. Date Re9ue/Date Received 2024-05-30 63
  9. 9. The particle sorting module according to any one of claims 1 to 8, further comprising a sample agitator.
  10. 10. The particle sorting module according to any one of claims 1 to 9, wherein the 5 module is sterile.
  11. 11. A system comprising: a particle sorting module according to any one of claims 1 to 1 O; a sample input module fluidically coupled to an inlet at the proximal end of the 10 particle sorting module housing; and a waste tank fluidically coupled to an outlet from the particle sorting module.
  12. 12. The system according to claim 11, further comprising a register for coupling with the aligner on the housing of the particle sorting module.
  13. 13. A method comprising: irradiating with a light source a sample comprising particles in a flow stream in an interrogation region of a particle sorting module according to any one of claims 1 to 1 O; detecting light from the sample; and 14. 15. sorting the particles in the sample into two or more sample collection containers. The method according to claim 13, wherein the particles are cells. A kit comprising: a particle sorting module according to any one of claims 1 to 1 O; a sample input module; and a waste tank. Date Re9ue/Date Received 2024-05-30

Description

1 ENCLOSED DROPLET SORTER AND METHODS OF USING THE SAME CROSS-REFERENCE TO RELATED APPLICATIONS This application claims priority to the filing date of United States Provisional 5 Patent Application Serial No. 62/323,418, filed April 15, 2016, and United States Provisional Patent Application Serial No. 62/364,282, filed July 19, 2016. INTRODUCTION Flow-type particle sorting systems, such as sorting flow cytometers, are used to 10 sort particles in a fluid sam pie based on at least one measured characteristic of the particles. In a flow-type particle sorting system, particles, such as molecules, analytebound beads, or individual cells, in a fluid suspension are passed in a stream by a detection region in which a sensor detects particles contained in the stream of the type to be sorted. The sensor, upon detecting a particle of the type to be sorted, triggers a 15 sorting mechanism that selectively isolates the particle of interest. Particle sensing typically is carried out by passing the fluid stream by a detection region in which the particles are exposed to irradiating light, from one or more lasers, and the light scattering and fluorescence properties of the particles are measured. Particles or components thereof can be labeled with fluorescent dyes to facilitate 20 detection, and a multiplicity of different particles or components may be simultaneously detected by using spectrally distinct fluorescent dyes to label the different particles or components. Detection is carried out using one or more photosensors to facilitate the independent measurement of the fluorescence of each distinct fluorescent dye. One type of flow-type particle sorting system is the electrostatic sorting type. In 25 an electrostatic sorter, a fluid suspension is jetted from a nozzle and vibrated to break the stream into uniform discrete drops. The sorting mechanism includes a drop charging means connected to the stream to charge drops containing a particle of the type to be sorted with an electrical charge as it breaks off from the jet stream. The stream of drops is passed through a transverse electrostatic field established by a pair of oppositely 30 charged deflection plates. Charged drops containing a particle of the type to be sorted are deflected in a direction and in an amount related to the polarity and magnitude of the drop charge and are collected in distinct collection receptacles. Uncharged drops are Date Re9ue/Date Received 2023-07-31 WO 2017/180325 PCT/0S2017/024609 2 not deflected passing through the electrostatic field and are collected by a central receptacle. SUMMARY Aspects of the present disclosure include a particle sorting module for sorting particle components of a sample, such as cells in a biological sample. Particle sorting modules according to certain embodiments include an enclosed housing having an aligner for coupling the housing with a particle sorting system, a flow cell nozzle positioned at the proximal end of the housing, a sample interrogation region in fluid 10 communication with the orifice of the flow cell nozzle and a droplet deflector. A particle sorting system and methods for separating components of a sample as well as kits, including one or more particle sorting module, suitable for coupling with a particle sorting system and for practicing the subject methods are also provided. In embodiments, the enclosed housing is configured for coupling with a particle 15 sorting system with one or more aligners. In some embodiments, the aligner includes one or more protrusions, such as balled tipped pin protrusions to align and couple the particle sorting module to the particle sorting system. The particle sorting module may also include a sample inlet and a sheath fluid inlet at the proximal end of the housing. The particle sorting module may be configured with electrical connectors on the housing. 20 In some embodiments, the particle sorting module also includes two or more sample collection containers. The sample collection containers may be fastened to the housing, such as being screw threaded with the housing. The particle sorting module may also include a cuvette, such as a glass or plastic cuvette positioned in the sample interrogation region. In some embodiments, the cuvette is positioned in the sample 25 interrogation region with an adhesive. In other embodiments, the cuvette is co-molded with the housing. Aspects of the present disclosure also include systems for sorting particle components of a sample, such as cells in a biological sample. Systems according to certain embodiments include one or more particle sorting modules, a sample input 30 module fluidically coupled to an inlet at the proximal end of the particle sorting module and a waste tank fluidically coupled to an outlet from the particle sorting module. In embodiments, the system is configured to be coupled with one or more particle sorting module. In some embodiments, the system includes a register for coupling with the WO 2017/1803