CA-3054119-C - CELL TREATMENT DEVICE, SUSPENSION CULTURE VESSEL AND STEM CELL INDUCTION METHOD

Abstract

This cell treatment device is provided with: a factor introduction device 30 for introducing a pluripotent induction factor into cells so as to prepare induction factor-introduced cells; and a reprogramming suspension culture vessel for culturing the induction factor-introduced cells that have been prepared by the factor introduction device 30.

Inventors

• Koji Tanabe
• Kenta SUTO
• Ryoji HIRAIDE

Assignees

• I PEACE, INC.
• Koji Tanabe

Dates

Publication Date

20260505

Application Date

20180223

Priority Date

20170224

Claims (18)

1. CLAIMS 1. A cell treatment device comprising: a factor introducing device that introduces a pluripotency inducing factor into cells to create inducing factor-introduced cells, a suspension culture vessel comprising a semipermeable membrane, for suspension culturing of the inducing factor-introduced cells created by the factor introducing device, to induce induced pluripotent stem cells from the inducing factor-introduced cells, and a fluid channel for feeding the inducing factor-introduced cells from the factor introducing device to the suspension culture vessel, wherein the suspension culture vessel further comprises a container in which the semipermeable membrane is placed, and wherein the inducing factor-introduced cells created by the factor introducing device are fed into the suspension culture vessel.
2. 2. The cell treatment device according to claim 1, wherein the inducing factor-introduced cells are reprogrammed inside the suspension culture vessel.
3. 3. The cell treatment device according to claim 1, wherein the induced cells are reprogrammed and expanded inside the suspension culture vessel.
4. 4. The cell treatment device according to claim 1, wherein the suspension culture vessel further comprises a drive unit for agitation of the culture medium in the container.
5. 5. The cell treatment device according to claim 4, wherein an agitating member is provided in the container.
6. 6. The cell treatment device according to claim 5, wherein the drive unit rotates the agitating member by magnetic force.
7. 7. The cell treatment device according to claim 1, wherein the container has a helical groove provided on its inner surface, and Date Re9ue/Date Received 2024-01-12 wherein the cell treatment device further comprises a pump that supplies culture medium into the container so that the culture medium flows along the groove inside the container.
8. 8. The cell treatment device according to any one of claims 4 to 7, wherein the inducing factor-introduced cells created by the factor introducing device are fed into the semipermeable membrane of the suspension culture vessel.
9. 9. A cell treatment device comprising: a factor introducing device that introduces a pluripotency inducing factor into cells to create inducing factor-introduced cells, a suspension culture vessel comprising a semipermeable membrane, for suspension culturing of the inducing factor-introduced cells created by the factor introducing device, to induce induced pluripotent stem cells from the inducing factor-introduced cells, and a fluid channel for feeding the inducing factor-introduced cells from the factor introducing device to the suspension culture vessel, wherein the suspension culture vessel comprises: a container in which the semipermeable membrane is placed with the inducing factorintroduced cells and culture medium accommodated around the periphery of the semipermeable membrane, and a pump that supplies culture medium into the semipermeable membrane so as to create a flow of the culture medium inside the semipermeable membrane.
10. 10. The cell treatment device according to claim 9, wherein the inducing factor-introduced cells created by the factor introducing device are fed outside of the semipermeable membrane inside the container.
11. 11. A cell treatment device comprising: a factor introducing device that introduces a pluripotency inducing factor into cells to create inducing factor-introduced cells, a suspension culture vessel comprising a semipermeable membrane, for suspension culturing of the inducing factor-introduced cells created by the factor introducing device, to induce induced pluripotent stem cells from the inducing factor-introduced cells, and 41 Date Re9ue/Date Received 2024-01-12 a fluid channel for feeding the inducing factor-introduced cells from the factor introducing device to the suspension culture vessel, wherein the suspension culture vessel comprises: a container in which the inducing factor-introduced cells and culture medium are placed, the container being provided with a helical groove provided on its inner surf ace, and a pump that supplies culture medium into the container so that the medium flows along the groove inside the container.
12. 12. The cell treatment device according to claim 11, wherein the inducing factor-introduced cells created by the factor introducing device are fed into the container of the suspension culture vessel.
13. 13. The cell treatment device according to any one of claims 4 to 12, wherein the culture medium is liquid medium.
14. 14. The cell treatment device according to any one of claims 4 to 12, wherein the culture medium is gel medium.
15. 15. A stem cell induction method that includes: introducing a pluripotency inducing factor into cells to create inducing factor-introduced cells, and suspension culturing the inducing factor-introduced cells in a semipermeable membrane while nutrients are supplied to the inducing factor-introduced cells through the semipermeable membrane to induce induced pluripotent stem cells from the inducing factor-introduced cells and to form a floating cell mass from the induced pluripotent stem cells.
16. 16. The stem cell induction method according to claim 15, wherein the suspension culture is agitation culture.
17. 17. The stem cell induction method according to claim 15 or 16, wherein liquid medium is used during suspension culture of the inducing factor-introduced cells. 42 Date Re9ue/Date Received 2024-01-12
18. 18. The stem cell induction method according to claim 15 or 16, wherein gel medium is used during suspension culture of the inducing factor-introduced cells. 43 Date Re9ue/Date Received 2024-01-12

Description

CELL TREATMENT DEVICE, SUSPENSION CULTURE VESSEL AND STEM CELL INDUCTION METHOD FIELD [0001] The present invention relates to cell technology, and specifically to a cell treatment device, a suspension culture vessel and a stem cell induction method. BACKGROUND [0002] Embryonic stem cells (ES cells) are stem cells established from early embryos of humans or mice, for example. ES cells are pluripotent, being capable of differentiating into all cells in the body. At the current time, human ES cells are able to be used in cell transplantation therapy for numerous diseases including Parkinson's disease, juvenile onset diabetes and leukemia. However, certain barriers exist against transplantation of ES cells. In particular, transplantation of ES cells can provoke immunorejection similar to the rejection encountered after unsuccessful organ transplantation. Moreover, there are many ethical considerations as well as critical and dissenting opinions against the use of ES cell lines that have been established by destruction of human embryos. [0003] It was against this background that Professor Shinya Yamanaka of Kyoto University successfully established a line of induced pluripotent stem cells (iPS cells) by transferring four genes: OCTI/4, KLF4, c-MYC and SOX2, into somatic cells. Forth.is, Professor Yamanaka received the Nobel Prize in Physiology or Medicine in 2012 (see PTLs 1 and 2, for example). iPS cells are ideal pluripotent cells which are free of issues of rejection or ethical problems. Therefore, iPS cells are considered promising for use in cell transplantation therapy. [CITATION LIST] 1 [PATENT LITERATURE] [0004] [PTL 1] Japanese Patent Publication No. 4183742 [PTL 2] Japanese Unexamined Patent Publication No. 2014-114997 SUMMARY [TECHNICAL PROBLEM] [0005] Devices and methods allowing efficient culturing of not only iPS cells but many other different types of cells are desired. It is therefore one object of the present invention to provide a cell treatment device, a suspension culture vessel and a stem cell induction method. [SOLUTION TO PROBLEM] [0005a] In an aspect, there is provided a cell treatment device comprising: a factor introducing device that introduces a pluripotency inducing factor into cells to create inducing factorintroduced cells, a suspension culture vessel comprising a semipermeable membrane, for suspension culturing of the inducing factor-introduced cells created by the factor introducing device, to induce induced pluripotent stem cells from the inducing factor-introduced cells, and a fluid channel for feeding the inducing factor-introduced cells from the factor introducing device to the suspension culture vessel, wherein the suspension culture vessel further comprises a container in which the semipermeable membrane is placed, and wherein the inducing factorintroduced cells created by the factor introducing device are fed into the suspension culture vessel. [0005b] In another aspect, there is provided a cell treatment device comprising: a factor introducing device that introduces a pluripotency inducing factor into cells to create inducing factorintroduced cells, a suspension culture vessel comprising a semipermeable membrane, for suspension culturing of the inducing factor-introduced cells created by the factor introducing device, to induce induced pluripotent stem cells from the inducing factor-introduced cells, and a fluid channel for feeding the inducing factor-introduced cells from the factor introducing device to the suspension culture vessel, wherein the suspension culture vessel comprises: a container in which the semipermeable membrane is placed with the inducing factor-introduced cells and 2 Date Re9ue/Date Received 2024-01-12 culture medium accommodated around the periphery of the semipermeable membrane, and a pump that supplies culture medium into the semipermeable membrane so as to create a flow of the culture medium inside the semipermeable membrane. [0005c] In another aspect, there is provided a cell treatment device comprising: a factor introducing device that introduces a pluripotency inducing factor into cells to create inducing factorintroduced cells, a suspension culture vessel comprising a semipermeable membrane, for suspension culturing of the inducing factor-introduced cells created by the factor introducing device, to induce induced pluripotent stem cells from the inducing factor-introduced cells, and a fluid channel for feeding the inducing factor-introduced cells from the factor introducing device to the suspension culture vessel, wherein the suspension culture vessel comprises: a container in which the inducing factor-introduced cells and culture medium are placed, the container being provided with a helical groove provided on its inner surface, and a pump that supplies culture medium into the container so that the medium flows along the groove inside the container. [0005d] In another aspect, there is provided a stem cell induction method that includes: intro