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CN-108137658-B - Maize plants producing enhanced resistance to northern leaf blight

CN108137658BCN 108137658 BCN108137658 BCN 108137658BCN-108137658-B

Abstract

Provided herein are compositions and methods for producing maize plants that exhibit resistance to northern leaf blight. Isolated polynucleotides encoding polypeptides conferring resistance to northern leaf blight, polynucleotide constructs comprising such polynucleotides, and maize plants comprising the polynucleotide constructs are provided. The method comprises expressing an isolated polynucleotide in a maize cell via standard transformation methods and obtaining a maize plant from the maize cell.

Inventors

  • Z .hou
  • A.L. la la
  • B.LI
  • G.M. Tabor

Assignees

  • 先锋国际良种公司
  • 先锋国际良种公司

Dates

Publication Date
20260421
Application Date
20161014
Priority Date
20151016

Claims (15)

  1. 1. A polynucleotide construct comprising an isolated polynucleotide selected from the group consisting of the nucleotide sequences set forth in SEQ ID NO.1 (PH 4GP c-DNA), SEQ ID NO. 3 (PH 1W2 cDNA) and SEQ ID NO. 9 (PH 4GP genomic sequence), Wherein the isolated polynucleotide is operably linked to a promoter or, in the case of SEQ ID NO. 9, comprises a promoter.
  2. 2. The polynucleotide construct of claim 1, wherein the polynucleotide construct further comprises one or more additional heterologous nucleic acid sequences encoding a polypeptide selected from the group consisting of a disease resistance conferring polypeptide, a herbicide resistance conferring polypeptide, an insect resistance conferring polypeptide, a polypeptide involved in carbohydrate metabolism, a polypeptide involved in fatty acid metabolism, a polypeptide involved in amino acid metabolism, a polypeptide involved in plant development, a polypeptide involved in plant growth regulation, a polypeptide involved in yield improvement, a polypeptide involved in drought resistance, a polypeptide involved in cold resistance, a polypeptide involved in heat resistance, and/or a polypeptide involved in salt resistance, wherein each heterologous nucleic acid sequence is operably linked to a promoter.
  3. 3. The polynucleotide construct of claim 2, wherein the polypeptide that confers disease resistance is a polypeptide that confers northern leaf blight NLB resistance.
  4. 4. The polynucleotide construct of claim 3, wherein the polypeptide that confers resistance to northern leaf blight is a polypeptide consisting of the amino acid sequences set forth in SEQ ID NOs 11 or 12.
  5. 5. A method for producing a maize plant cell comprising the polynucleotide construct of claim 1.
  6. 6. A method for producing a maize plant comprising the maize plant cell of claim 5.
  7. 7. A method for producing a maize plant that exhibits resistance to northern leaf blight NLB, the method comprising: a) Expressing a heterologous polynucleotide construct comprising a polynucleotide selected from the group consisting of the nucleotide sequences set forth in SEQ ID NO. 1 (PH 4GP c-DNA), SEQ ID NO. 3 (PH 1W2 cDNA) and SEQ ID NO. 9 (PH 4GP genomic sequence) in a regenerable maize plant cell, wherein the polynucleotide is operably linked to at least one regulatory sequence or, in the case of SEQ ID NO. 9, at least one regulatory sequence, and B) Generating a maize plant that exhibits resistance to northern leaf blight, wherein the maize plant comprises the heterologous polynucleotide construct in its genome.
  8. 8. The method of claim 7, wherein the at least one regulatory sequence is a promoter.
  9. 9. The method of claim 7, wherein the at least one regulatory sequence is a terminator.
  10. 10. The method of claim 7, wherein the regulatory sequence is native to maize.
  11. 11. The method of claim 7, wherein the regulatory sequence is native to the Ht1 gene.
  12. 12. The method of claim 7, wherein the polynucleotide construct comprises one or more additional heterologous nucleic acid sequences encoding a polypeptide selected from the group consisting of a disease resistance conferring polypeptide, a herbicide resistance conferring polypeptide, an insect resistance conferring polypeptide, a polypeptide involved in carbohydrate metabolism, a polypeptide involved in fatty acid metabolism, a polypeptide involved in amino acid metabolism, a polypeptide involved in plant development, a polypeptide involved in plant growth regulation, a polypeptide involved in yield improvement, a polypeptide involved in drought resistance, a polypeptide involved in cold resistance, a polypeptide involved in heat resistance, and/or a polypeptide involved in salt resistance, wherein each heterologous nucleic acid sequence is operably linked to a promoter.
  13. 13. The method of claim 12, wherein the polypeptide that confers disease resistance is a polypeptide that confers resistance to northern leaf blight NLB.
  14. 14. The method of claim 13, wherein the polypeptide that confers resistance to northern leaf blight is a polypeptide consisting of the amino acid sequences set forth in SEQ ID NOs 11 or 12.
  15. 15. A method of obtaining a maize plant that exhibits resistance to northern leaf blight NLB, the method comprising: a) Crossing a maize plant produced by the method of claim 7 with a maize plant that does not comprise said polynucleotide construct in its genome; b) Obtaining a progeny plant that exhibits resistance to northern leaf blight, wherein the progeny plant comprises the polynucleotide construct in its genome.

Description

Maize plants producing enhanced resistance to northern leaf blight Cross Reference to Related Applications The application claims the benefit of U.S. provisional application No. 62/242,691 filed on 10 months 16 of 2015, the entire contents of which are hereby incorporated by reference. Technical Field The present disclosure relates to compositions and methods useful in producing maize plants with enhanced resistance to northern leaf blight. Electronically submitted references to sequence listings An official copy of this sequence listing was submitted electronically via the EFS-Web as an ASCII formatted sequence listing, with a file name "20160228_BB2396PCT_sequence listing. Txt", created at 28, 9, 2016, and having a 65 kilobyte size, and submitted concurrently with this specification. The sequence listing included in this ASCII formatted file is part of this specification and is incorporated herein by reference in its entirety. Background Northern leaf blight (northern leaf blight, NLB) induced by the fungal pathogen Leptosporum maydis (Exserohilum turcicum) (formerly known as Helminthosporum megaterium (Helminthosporlum turcicum)) is a severe maize leaf blight in many tropical and temperate environments. Symptoms can range from cigar lesions on the lower leaves to complete destruction of the leaves, reducing the amount of leaf surface area available for photosynthesis. The reduced photosynthetic capacity results in a lack of carbohydrates required for grain filling, which can affect grain yield. Because of the long open time and moderate temperatures, there is a particularly favorable climate for northern leaf blight in the mid-sea pulling areas of the tropics (about 900-1600m above sea level). However, in temperate environments (e.g., in the united states), northern leaf blight may also produce 30% -50% loss during wet seasons, particularly if infection is determined on the upper leaves of plants during the silking period. The most effective and preferred method of controlling northern leaf blight is to plant resistant hybrids. Several species or races (race) of the species Rhizoctonia cerealis exist in nature, allowing the grower to select for two hybrids, a partially resistant hybrid that provides a low level of broad spectrum protection against multiple races, and a race-specific resistant hybrid that provides protection against a particular race. Genetic sources of resistance to Rhizoctonia solani have been described, and four Rhizoctonia solani resistance loci have been identified, ht1, hr2, ht3, and Htn1. Gene Ht1 is located on the long arm of chromosome 2, where gene Ht1 is closely linked to umc36 (Coe, E.H. et al (1988), corn and Corn Improvement [ Maize and Maize modifications ], 3 rd edition, pages 81-258), sgcr506 (Gupta, M. et al (1989) Maize Genet. Coop. Newsl. [ Maize genetics news communications ]63,112), umc150B (Bentolila, S. et al (1991) Ther. Appl. Genet. [ theory and applied genet. ], 82:393-398), and pic18a (Collins et al (1998) Molecular Plant-microorganism interactions ], 11:968-978), and immediately flanking umc22 and umc122 (Li et al (1998) HEREDITAS [ genetic ], 129:101-106). Gene Ht2 is located in the umc48-umc89 region on the long arm of chromosome 8 (Zaitlin et al (1992) Maize Genet. Coop. Newsl. [ Maize genetics collaboration news communication ],66, 69-70), and gene Ht3 maps to the vicinity of bnlg1666 on chromosome 7 (VAN STADEN, D et al (2001) Maize Genetics Conference Abstracts [ Maize genetics conference abstract ] 43:P134). Htn1 gene is located on chromosome 8, approximately 10cM from Ht2 and 0.8cM from RFLP marker umc117 (Simcox and Bennetzen (1993) Maize Genet. Coop. Newl. [ Maize genetics Cooperation news communication ]67,118-119; simcox and Bennetzen (1993) phytology [ plant pathology ], 83:1326-1330). Methods of controlling northern leaf blight by reducing fungal inoculums require additional time and resources for a portion of the farmer and, in addition, may have adverse effects on the environment. This makes the planting of resistant hybrids more attractive to farmers and the public. Accordingly, it would be desirable to provide compositions and methods for producing maize plants with enhanced resistance to northern leaf blight. Disclosure of Invention Compositions and methods for producing maize plants that exhibit resistance to northern leaf blight, whether the resistance is newly conferred or enhanced, are described herein. Isolated polynucleotides are described herein that can be used to produce maize plants that exhibit resistance to northern leaf blight. The isolated polynucleotide may be selected from the group consisting of (a) a nucleotide sequence set forth in SEQ ID NO:1 (PH 4GP c-DNA), SEQ ID NO:3 (PH 1W2 cDNA), or SEQ ID NO:9 (PH 4GP genomic sequence), (b) a nucleotide sequence encoding a CC-NB-LRR polypeptide having an amino acid sequence comprising the amino acid sequence set forth in SEQ ID NO:10 based on a CLUSTALW alignment method using defaul