CN-108935981-B - Effect of nicotinamide addition to lamb glycolipid metabolism in perinatal milk goats and mechanism thereof

Abstract

The invention discloses the influence and mechanism of the addition of nicotinamide to the metabolism of lamb glycolipid of a perinatal milk goat, starts from the addition of nicotinamide to a parent, researches the influence and mechanism of the nicotinamide to the morphological development of lamb intestinal tracts and the expression of nutrient transport vectors, glucose metabolism and blood biochemical indexes and fat metabolism, and discovers that the addition of nicotinamide to the perinatal milk goat can improve the glucose absorption of the lamb intestinal tracts, inhibit the liver gluconeogenesis of the lamb, promote the liver lipolysis of the liver, reduce the liver injury, promote the abdominal fat deposition and the morphological development of the intestinal tracts of the lamb, and promote the growth and development of the lamb, and the research result provides theoretical basis for regulating the growth and development of the lamb.

Inventors

• CAO YANGCHUN
• YAO JUNHU
• WANG LAMEI
• WEI XIAOSHI
• ZHAO HUIHUI
• HE JIAJUN
• YIN QINGYAN
• CAI CHUANJIANG
• XU XIURONG

Assignees

• 西北农林科技大学

Dates

Publication Date

20260505

Application Date

20180605

Claims (3)

1. 1. The application of adding nicotinamide into the perinatal milk goats in promoting the intestinal morphological development of the lambs and improving the intestinal digestion and absorption of the lambs is characterized in that the nicotinamide is added from 21 d to 28 d after the perinatal milk goats are prenatal; Increasing the height of the lamb duodenum villus and the ratio V/C of the height of the duodenum villus to the depth of the crypt, increasing the ratio V/C of the height of the lamb jejunum villus to the depth of the crypt, increasing the height of the lamb ileum villus and increasing the ratio V/C of the height of the ileum villus to the depth of the crypt, thereby promoting the morphological development of the lamb and improving the digestion and absorption capacity of the lamb intestinal tract; promoting the expression of the lamb jejunum glucose transport vectors GLUT2 and SGLT1 and the expression of the ileum GLUT2, and further promoting the transportation and absorption of the lamb jejunum glucose.
2. 2. Use according to claim 1, characterized in that nicotinamide is added by direct oral feeding of nicotinamide or ration.
3. 3. The use according to claim 2, characterized in that it is fed 1 time each in the morning and in the evening, 2.5g each time.

Description

Effect of nicotinamide addition to lamb glycolipid metabolism in perinatal milk goats and mechanism thereof Technical Field The invention belongs to the field of animal nutrition, and particularly relates to an influence of adding nicotinamide into a milk goat in a perinatal period on the metabolism of offspring lamb glycolipid. Background Perinatal is a special physiological period of the dairy animals, including late gestation and pre-lactation, generally referring to 21 days before birth to 21 days after birth. The metabolism of tissues and organs such as placenta, mammary gland, fat, muscle, liver and gastrointestinal tract of the dairy animals during pregnancy and lactation peak period can be greatly changed so as to supply glucose and amino acids for mother and child nutrition distribution and fetal development, and supply free fatty acids (FREE FATTY ACID, FFA) such as acetic acid, propionic acid and butyric acid for liver and mammary gland for gluconeogenesis and fat synthesis so as to meet the lactation energy and fat requirements. Changes in fetal nutrition and endocrine status may cause metabolic, structural, physiological changes in offspring, and changes are permanent and heritable, even causing diseases in offspring (Metcoff et al 1980). Environmental and nutritional effects of fetuses and newborns even affect post-adult productivity. The fetus increases weight rapidly 6 weeks before delivery, and the mother is the sole source of nutrition for the fetus. Therefore, ensuring maternal nutrition in the late gestation period is critical to fetal development. Studies show that vitamin A is added 1 month before delivery of the ewes to promote the survival rate and the production performance of the lambs (Eldaim et al.2015), vitamin E is added to the ewes in gestation to reduce the stillbirth rate (Donnem et al.2015), and the ewes are fed in late gestation and early lactation to improve the birth weight and the 60-day body weight of the lambs (Idris et al.2010). Therefore, the nutrition of the mother in the perinatal period and the lactation early period is important for the growth and development of the fetus and the postnatal early period, and the nutrition of the mother in the perinatal period and the lactation early period is ensured to be sufficient, so that the growth and development of offspring can be promoted, and the production performance is improved. Niacin and nicotinamide are two forms of vitamin B3 that are interchangeable in vivo, nicotinamide being one of the major precursors of nicotinamide adenine dinucleotide (NAD +) and nicotinamide adenine dinucleotide phosphate (NADP +). Niacin mainly originates from plants, nicotinamide mainly originates from animals, but niacin in grains mainly exists in a combined state, and the utilization efficiency is low. The main sources of nicotinic acid in rumen of ruminant are (1) nicotinic acid in feed, (2) the rumen microorganism synthesizes the nicotinic acid, and (3) tryptophan is converted into the nicotinic acid in liver through canine uric acid, but the conversion efficiency is lower, and the tryptophan belongs to essential amino acid, and the proportion of the part is smaller. The current study on nicotinamide in terms of glycometabolism is that supplementation of perinatal cows with niacin significantly reduced expression of hepatic glucose transport carrier 2 (glucose transporter, GLUT 2) (Kinoshita et al 2016), and that addition of nicotinamide regulates glycometabolism and NAD-sirtuin pathway and may be associated with altered mitochondrial function in obese and diabetic mice (Yang et al 2013) perinatal cows with niacin alters expression of hepatic glycometabolism genes, possibly associated with FoxO1 (Asako Kinoshita et et al 2015). Nicotinamide has been studied in terms of fat metabolism, as a functional micronutrient critical to energy metabolism, and long-term treatment of MSC cells with nicotinamide promotes adipogenesis (Shapiro et al 2016). Liu et al (2009) found that nicotinamide can control lipolysis and energy balance of bovine fat precursor cells, and that nicotinamide promoted hepatocyte energy metabolism in a certain concentration range (Cang Kun et al 2009; 2013 of adult sea building et al) and improved lipid metabolism of stressed cows (Sun Xianzhi et al 2015). Nicotinic acid is a good lipid-lowering drug at pharmacological doses, which lowers low-density lipoprotein (LDL) levels and increases high-density lipoprotein (HDL) levels. Supplementing niacin to limited-feeding Holstein cows reduces blood non-esterified fatty acid (NEFA) level (Pires et al.2007), supplementing 12g/d rumen protection niacin to perinatal cows, inhibiting lipolysis, reducing milk energy output, improving perinatal energy balance, but having no influence on feed intake (Yuan et al.2012), supplementing 24g/d niacin can reduce blood NEFA level of perinatal cows, inhibit lipolysis (Morey et al.2011), supplementing 1000mg/kg niacin to Chinese hybrid fatte