CN-110506831-B - Fermented compound feed for preventing diarrhea of piglets and preparation and application methods thereof

Abstract

The invention discloses a fermented compound feed for preventing diarrhea of piglets and a preparation method and an application method thereof. Premixing corn, bean pulp and bran according to a mass ratio of 4.5:4.5:1.0, adding ZJUAF-2 fermentation liquor with a mass percentage of 10% and ZJUAF-4 fermentation liquor with a mass percentage of 10%, adding 40% of water, fermenting at an initial temperature of 25-30 ℃, fermenting for 48-72 hours, turning over once every 5-8 hours, and obtaining the corn-bean pulp type fermented compound feed when the pH of the feed is reduced to 4.0. Corn, soybean meal and bran adopt a bacillus subtilis and a pediococcus pentosaceus for mixed fermentation, the contents of beta-conglycinin and glycinin are reduced by 71.94% and 82.86%, neutral washing fibers are reduced by 38.93%, the contents of flatulence factors raffinose and stachyose are respectively reduced by 42.22% and 36.60%, the contents of phytic acid phosphorus are reduced by 37.50%, besides the obvious reduction of the anti-nutritional factor contents, the lactic acid content is improved by 3.5 times, the acid soluble protein is improved by 6 times, and the fermented feed is used for feeding piglets, and the control effect on diarrhea of the piglets is equivalent to that of feeding antibiotics.

Inventors

• ZHANG YU
• WANG YIZHEN
• LU ZEQING
• HAO LIHONG
• JIANG ZIPENG
• SU WEIFA
• WANG CHENG
• SHI CHANGYOU
• WANG FENGQIN

Assignees

• 浙江大学

Dates

Publication Date

20260508

Application Date

20190717

Claims (1)

1. 1. A fermented compound feed for preventing diarrhea of piglets is characterized in that corn, soybean meal and bran are mixed and fermented by adopting bacillus subtilis and pediococcus pentosaceus, the content of beta-conglycinin and glycinin is reduced by 71.94% and 82.86%, neutral washing fiber is reduced by 38.93%, the content of flatulence factor raffinose and stachyose is respectively reduced by 42.22% and 36.60%, the content of phytic acid phosphorus is reduced by 37.50%, except that the content of anti-nutritional factors is obviously reduced, the content of lactic acid is increased by 3.5 times, and the content of acid soluble protein is increased by 6 times; the bacillus subtilis belongs to bacillus subtilis subspecies, bacillus subtilis subsp. Subilis is named ZJUAF-2, is preserved in China general microbiological culture Collection center, and has a preservation date of 2016, 8 and 8 days, and a preservation number of CGMCC NO:12824, and the pediococcus pentosaceus belongs to pediococcus pentosaceus, pediococcus pentosaceus, is named ZJUAF-4, is preserved in China general microbiological culture collection center, has a preservation date of 2018, 11 and 21 days, and has a preservation number of CGMCC NO:16767; The preparation method of the fermented compound feed comprises the steps of premixing corn, soybean meal and bran according to a mass ratio of 4.5:4.5:1.0, adding ZJUAF-2 fermentation liquor and 10% ZJUAF-4 fermentation liquor which are in mass percent, adding 40% water, wherein the fermentation starting temperature is 25-30 ℃, the fermentation time is 48-72 h, turning the feed once every 5-8 h, and obtaining the corn-soybean meal type fermented compound feed when the pH of the feed is reduced to 4.0; The ZJUAF-2 fermentation liquor is prepared by culturing ZJUAF-2 to OD 600 by adopting an LB (liquid-solid) culture medium to reach 1.2, inoculating the culture medium into the fermentation liquor culture medium according to the volume ratio of 5.0%, wherein the culture medium comprises, by mass, 2.0% of glycerol, 3.0% of soybean meal powder, 0.03% of NaH 2 PO 4 0.4%、K 2 HPO 4 , 0.5-1.0% of bran and the balance of water, and culturing the fermentation liquor at 30 ℃ at 200 r/min for 24 h until the neutral protease activity reaches 600U/mL; The ZJUAF-4 fermentation broth is prepared by culturing with MRS culture medium until OD 600 reaches 1.4-1.7, inoculating 0.1% of inoculating fermentation broth culture medium according to volume ratio, wherein the culture medium comprises yeast powder 0.5-2.0%, glucose 0.5-2.0%, tomato powder 0.5-1.0%, KH 2 PO 4 0.1-0.5%, and water in balance, and performing stationary culture at 37deg.C for 24h until the bacterial concentration reaches 1.0X10 9 ~1.0×10 10 cfu/mL.

Description

Fermented compound feed for preventing diarrhea of piglets and preparation and application methods thereof Technical Field The invention relates to the field of microorganisms and fermentation, in particular to a fermented compound feed for preventing diarrhea of piglets and a preparation and application method thereof. Background Diarrhea is a significant cause of death in piglets. After weaning, as the digestive tract and the enzyme system thereof are not developed well, the digestion and utilization rate of nutrient substances in the feed by piglets is reduced, thereby providing favorable conditions for the reproduction of intestinal pathogens and further inducing diarrhea. In addition, when the feed is ingested, antigenic substances in the feed, such as antigenic proteins, can elicit systemic immune responses, inducing diarrhea. Therefore, the anti-nutritional factors of the feed are reduced, the digestion and utilization of the nutritional substances of the feed by the piglets are improved, and the diarrhea of the piglets is prevented. The biological fermentation effectively reduces feed anti-nutritional factors, improves the digestion and utilization rate of feed nutrients by animals, improves animal health, relieves diarrhea of piglets caused by antibiotic decrement, and becomes an important point of feed resource development in China. However, the existing biological fermentation feeds are various, and the quality is different due to different strains and fermentation processes. In addition, for cost reduction, unconventional feed materials are used for the production of fermented feeds. However, the non-conventional raw materials have no sound product standard and proper recommended addition amount, and lack residual evaluation (Zhang Zhenlei, 2017) of toxins and anti-nutritional factors in the non-conventional raw materials, so that the safety and effectiveness of the fermented product still need to be further demonstrated. Therefore, a safe and effective bio-fermented feed product is urgently required to be developed. Aiming at how to degrade the anti-nutritional factors in the feed, the applicant designs a fermented compound feed taking common plant raw materials such as corn, bean pulp, bran and the like as substrates in a patent ZL201610728880.3, so that potential toxins existing in unconventional raw materials are avoided. Meanwhile, a bacillus strain screened in the patent ZL201610728303.4 for efficiently degrading soybean antigen protein is combined to prepare a fermented compound feed with low soybean antigen protein, and the ileum digestibility of piglets is measured to prove that the bacillus strain can effectively improve the digestibility of feed amino acids (C.Shi, 2017). However, along with the increase of the single-batch fermentation scale to more than ten tons and the mixed use of multiple strains, the oxygen content in a fermentation system is rapidly reduced, anaerobic acidogenic bacteria are easy to form dominant bacterial groups, the acidification of the feed is accelerated, the activity of digestive enzymes secreted by microorganisms is reduced, the degradation level of anti-nutritional factors is reduced, and the quality of the fermented feed is reduced. The addition of the enzyme preparation to the feed can promote the decomposition of feed macromolecules and improve the feed digestibility (for example, in CN201811644468.9 and CN201811295681.3, the inventor adopts neutral protease to degrade protein raw materials), but the quality of the fermented feed is limited by the enzyme activity of the enzyme preparation product, and meanwhile, the production steps and the cost are increased. Therefore, the enzyme production performance of the strain is fully exerted, the substrate enzyme activity in the feed fermentation is improved, the anti-nutritional factors in the feed are effectively reduced, the diarrhea of piglets is prevented, and the production cost is reduced. Disclosure of Invention In order to overcome the defects of the prior art, the invention provides a fermented compound feed for preventing diarrhea of piglets and a preparation and application method thereof. The corn-soybean meal type fermented composite feed for preventing diarrhea of piglets comprises the steps of adopting a bacillus subtilis strain and a pediococcus pentosaceus strain for mixed fermentation of corn, soybean meal and bran, wherein the content of beta-conglycinin and glycinin is reduced by 71.94% and 82.86%, the content of neutral washing fiber is reduced by 38.93%, the content of flatulence factor raffinose and stachyose is respectively reduced by 42.22% and 36.60%, the content of phytic acid phosphorus is reduced by 37.50%, the content of anti-nutritional factor is obviously reduced, the lactic acid content is increased by 3.5 times, and the acid soluble protein is increased by 6 times; The bacillus subtilis belongs to bacillus subtilis subspecies, bacillus subtilis subsp. Subtilis is named ZJUA