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CN-113396917-A - Composition containing propionyl brassinolide and hypersensitivity protein

CN113396917ACN 113396917 ACN113396917 ACN 113396917ACN-113396917-A

Abstract

The invention discloses a composition containing propionyl brassinolide and hypersensitivity protein, which is characterized in that maleic anhydride is added into a three-neck flask provided with a reflux condenser tube and a thermometer, polyethylene glycol monomethyl ether is added, magneton stirring is started, p-toluenesulfonic acid is added to obtain an intermediate C, and the intermediate C and phenylpropylene are added into a four-neck flask; the propionyl brassinolide and the hypersensitive protein are reasonably compounded, so that the propionyl brassinolide can play a role in regulating the growth of plants, promote the growth of the plants and improve the yield of crops, the stability of pesticides is improved by adding the dispersing auxiliary, the sedimentation is not easy to occur, and the absorption efficiency of the plants is improved by adding the surface active auxiliary, so that the purposes of efficiently using the pesticides, protecting the environment and reducing the soil pressure are achieved.

Inventors

  • LIU YANHE
  • CHEN QI

Assignees

  • ANHUI RUNNONG TENGHUI BIOLOGICAL TECH CO LTD

Dates

Publication Date
20210917
Application Date
20210708
Priority Date
20210708

Claims (8)

  1. 1. The composition containing propionyl brassinolide and hypersensitivity protein is characterized by comprising the following components in parts by weight: propionyl brassinolide 0.001-5 parts, hypersensitivity protein 1-10 parts, solvent 5-20 parts, water 1-20 parts, dispersing auxiliary agent 1-10 parts, surface active auxiliary agent 1-5 parts; the dispersing aid is prepared by the following steps: s1: adding maleic anhydride into a three-neck flask provided with a reflux condenser tube and a thermometer, adding polyethylene glycol monomethyl ether, starting magneton stirring, controlling the temperature at 40-50 ℃, adding p-toluenesulfonic acid, heating to 110-120 ℃, and reacting for 2-3h to obtain an intermediate C; s2: adding the intermediate C and the phenylpropylene into a four-neck flask, dropwise adding 2-methoxybutenoic acid by using a constant flow pump while stirring, controlling the dropwise adding speed to be 1-2 drops/s, simultaneously dropwise adding an initiator by using the constant flow pump, controlling the dropwise adding speed to be 1-2 drops/s, controlling the temperature to be 120-130 ℃, reacting for 4-5h, and adding sodium hydroxide for neutralizing to be neutral to obtain the dispersing aid; the surface active auxiliary agent is prepared by the following steps: s3: adding epoxy chloropropane and sodium hydroxide solid into a three-neck flask provided with a constant-pressure dropping funnel and a thermometer, adding water and tetrabutyl ammonium bromide, stirring at normal temperature for 1-2h, adding diethylene glycol butyl ether into the constant-pressure dropping funnel for dropwise adding, controlling the dropwise adding rate to be 2-3 drops/s, reacting at normal temperature for 3-4h, washing with dichloromethane, filtering, collecting filtrate, reducing the pressure at normal temperature, and cooling to obtain an intermediate A; s4: adding phosphoric acid into a three-neck flask provided with a constant pressure dropping funnel and a thermometer, adding ether as a solvent, starting a magneton for stirring, adding the intermediate A into the constant pressure dropping funnel for dropwise adding, controlling the dropwise adding rate to be 1-2 drops/s, controlling the temperature to be 40-60 ℃, reacting for 6-7h, and reducing the pressure to obtain an intermediate B; s5: and adding the intermediate B into a beaker, adding triethanolamine for neutralization by using ethanol as a solvent, adjusting the pH value to 7-8, and performing suction filtration to obtain the surface active auxiliary agent.
  2. 2. The composition containing propionyl brassinolide and hypersensitivity protein according to claim 1, characterized in that said solvent is one or a mixture of more than two of ethyl acetate, dioctyl phthalate, cyclohexane, paraffin, ethanol, ethylene glycol, toluene, petroleum ether, dimethylformamide and acetone, soybean oil in any proportion.
  3. 3. The composition of claim 1, wherein the composition is formulated in a dosage form selected from the group consisting of a solution, an aqueous solution, a soluble powder, and a wettable powder.
  4. 4. The propionyl brassinolide and hypersensitivity protein-containing composition according to claim 1, wherein the mass of said p-toluenesulfonic acid in step S1 is 8% of the total mass of maleic anhydride and polyethylene glycol monomethyl ether, the molar ratio of said maleic anhydride to said polyethylene glycol is 1: 1.
  5. 5. the propionyl brassinolide and hypersensitivity protein composition according to claim 1, wherein said initiator is azobisisobutyronitrile in the step S2, and the molar ratio of said intermediate C, phenylpropenoic acid and 2-methoxybutenoic acid is 4: 1: 0.4.
  6. 6. the propionyl brassinolide and hypersensitivity protein-containing composition according to claim 1, wherein the ratio of the amount of said water to tetrabutylammonium bromide in step S3 is 1 mL: 0.12g, and the dosage ratio of the water to the dichloromethane is 1 mL: 40mL, wherein the molar ratio of the epichlorohydrin, the sodium hydroxide, the tetrabutylammonium bromide and the diethylene glycol monobutyl ether is 0.6: 0.6: 0.02: 0.1.
  7. 7. the propionyl brassinolide and hypersensitivity protein-containing composition according to claim 1, wherein said phosphoric acid to diethyl ether dosage ratio in step S4 is 3 g: 100mL, wherein the molar ratio of the phosphoric acid to the intermediate A is 1: 3.
  8. 8. the propionyl brassinolide and hypersensitivity protein-containing composition according to claim 1, wherein the amount ratio of said intermediate B to ethanol in step S5 is 1 g: 100 mL.

Description

Composition containing propionyl brassinolide and hypersensitivity protein Technical Field The invention relates to the field of pesticides, and in particular relates to a composition containing propionyl brassinolide and a hypersensitive protein. Background Plant hormones play an important role in the growth and development of plants, and the growth and development of the plants are regulated by applying the hormones or growth regulators outside the plants, for target plants, the plant growth regulators are exogenous non-nutritive chemical substances and can be conducted to action parts in the plants, and certain links of the life process of the plants can be promoted or inhibited at low concentration, so that the plant hormones promote the growth of the plants, improve the yield of the crops, increase the stress resistance of the crops and the like; propionyl brassinolide is an upgraded product of a brassinolide original drug, is a novel and efficient plant growth regulator, has longer drug effect period, more obvious and more stable effect besides the advantages of the brassinolide original drug, shows obvious effects of stress resistance, yield increase, quality improvement and the like in agricultural application, and is an excellent product which is rare for developing high-quality and efficient agriculture; the hypersensitive protein is a thermostable protein provided from fungi or bacteria, is composed of proteins with different molecular weights of 35-68KD, can induce and activate the resistance of plants to plant diseases and insect pests, regulate the metabolic system of plant growth, promote the plant growth, improve the crop yield and improve the crop quality, is a novel biological pesticide, is non-toxic and residue-free, and is environment-friendly; the pesticide composition and the pesticide are reasonably compounded and mutually cooperated, so that a more excellent effect can be achieved, but the existing pesticide composition has low absorption efficiency of plants after application, high-concentration pesticide is required to be sprayed or applied for many times, so that resource waste and soil unfriendliness are caused, and in the application process, the pesticide is easy to agglomerate, so that sedimentation is caused, the application concentration is not uniform, and the efficacy of the pesticide cannot be better exerted; to this end, we propose a composition containing propionyl brassinolide and a hypersensitivity protein. Disclosure of Invention In order to overcome the technical problems, the invention aims to provide a composition containing propionyl brassinolide and hypersensitivity protein: the propionyl brassinolide and the hypersensitive protein are reasonably compounded to play a role in regulating the growth of plants, the propionyl brassinolide-hypersensitive protein-containing composite can strengthen the functions in plants, promote the growth of the plants and improve the yield of crops, the hypersensitive protein can be combined with receptor protein on the surfaces of the plants after being applied to the plants, the receptor protein of the plants can promote the synthesis of salicylic acid and jasmonic acid in the plants after receiving the signal conduction of activating protein, then the plant protection protein and other proteins related to disease resistance are synthesized by the salicylic acid or the jasmonic acid to achieve the effects of disease resistance and insect prevention, the stability of the pesticide is improved by adding the dispersing auxiliary agent, the sedimentation is not easy to occur, the absorption efficiency of the plants is improved by adding the surface active auxiliary agent, and therefore the purposes of high-efficiency pesticide use, environment. The purpose of the invention can be realized by the following technical scheme: a composition containing propionyl brassinolide and hypersensitivity protein comprises the following components in parts by weight: propionyl brassinolide 0.001-5 parts, hypersensitivity protein 1-10 parts, solvent 5-20 parts, water 1-20 parts, dispersing auxiliary agent 1-10 parts, surface active auxiliary agent 1-5 parts; the dispersing aid is prepared by the following steps: s1: adding maleic anhydride into a three-neck flask provided with a reflux condenser tube and a thermometer, adding polyethylene glycol monomethyl ether, starting magneton stirring, controlling the temperature at 40-50 ℃, adding p-toluenesulfonic acid, heating to 110-120 ℃, and reacting for 2-3h to obtain an intermediate C; the reaction principle is as follows: s2: adding the intermediate C and the phenylpropylene into a four-neck flask, dropwise adding 2-methoxybutenoic acid by using a constant flow pump while stirring, controlling the dropwise adding speed to be 1-2 drops/s, simultaneously dropwise adding an initiator by using the constant flow pump, controlling the dropwise adding speed to be 1-2 drops/s, controlling the temperature to be