CN-114557409-B - Turbot composite immunopotentiator, and preparation method and application thereof

CN114557409BCN 114557409 BCN114557409 BCN 114557409BCN-114557409-B

Abstract

The invention provides a turbot composite immunopotentiator, a preparation method and application thereof. The application of the composition containing a series of components in preparing the turbot immunopotentiator is disclosed, wherein the immunopotentiator can effectively improve the expression of turbot pro-inflammatory related genes, reduce the bacterial number of livers or intestinal tracts, enhance the sterilizing capacity of turbots, enhance the antioxidant stress capacity of turbots, relieve the liver injury of turbots after bacterial infection and relieve the tissue injury of turbots after bacterial infection. The immunopotentiator provided by the invention has the advantages of simple and easily available components, environment friendliness, low cost and ideal effect, and provides a new scheme for improving the cultivation level and the survival rate of marine fishes.

Inventors

YANG DAHAI
WANG ZHUANG
JIANG YU
LIU QIN
WANG PENGBO
WANG QIYAO
ZHANG YUANXING

Assignees

华东理工大学
上海纬中生物科技有限公司

Dates

Publication Date: 20260505
Application Date: 20220322

Claims (7)

1. The application of the composition in preparing turbot immunopotentiator comprises beta-glucan, mannooligosaccharide, astragalus polysaccharide, lecithin, fish oil and phytase; the composition comprises the following components in percentage by weight: The immunity enhancement is the enhancement of the capability of resisting the infection of the edwardsiella and the aeromonas salmonicida, reduces the bacterial quantity of liver or intestinal tissues of turbot after the infection of the edwardsiella and the aeromonas salmonicida, relieves the liver injury of the turbot after the infection of the edwardsiella and the aeromonas salmonicida, and relieves the intestinal tissue injury of the turbot after the infection of the edwardsiella and the aeromonas salmonicida; The immune enhancement is to improve the expression of turbot pro-inflammatory related genes after being infected by fish killing Edwardsiella and salmonicida aeromonas, wherein the pro-inflammatory related genes comprise il1b, il6 and tnf, improve the lysozyme activity of turbot tissues, improve the sterilizing capability of turbots and improve the lysozyme activity of livers or intestinal tracts.
2. The use according to claim 1, wherein the immunopotentiation is to increase the catalase activity of the liver or intestinal tissue of turbot and to increase the antioxidant stress capacity of turbot.
3. The use according to claim 1, wherein the composition comprises, in weight percent:
4. The use according to claim 1, wherein the turbot is an edwardsiella fish-killing (EDWARDSIELLAPISCICIDA) susceptible fish and an aeromonas salmonicida (Aeromonas salmonicidas) susceptible fish.
5. The application of the composition in preparing fish feed, wherein the fish feed is turbot feed, is characterized in that the composition is a turbot immunopotentiator, and comprises the following components in percentage by weight: The immunity enhancement is the enhancement of the capability of resisting the infection of the edwardsiella and the aeromonas salmonicida, reduces the bacterial quantity of liver or intestinal tissues of turbot after the infection of the edwardsiella and the aeromonas salmonicida, relieves the liver injury of the turbot after the infection of the edwardsiella and the aeromonas salmonicida, and relieves the intestinal tissue injury of the turbot after the infection of the edwardsiella and the aeromonas salmonicida; The immune enhancement is to improve the expression of turbot pro-inflammatory related genes after being infected by fish killing Edwardsiella and salmonicida aeromonas, wherein the pro-inflammatory related genes comprise il1b, il6 and tnf, improve the lysozyme activity of turbot tissues, improve the sterilizing capability of turbots and improve the lysozyme activity of livers or intestinal tracts.
6. The use according to claim 5, wherein the composition comprises, in weight percent: the components of the composition are mixed in water.
7. A method for preparing a composition, namely turbot immunopotentiator, comprises the steps of mixing beta-glucan, mannooligosaccharide, astragalus polysaccharide, lecithin and phytase, adding fish oil into the mixture, adding purified water into the mixture, and homogenizing and emulsifying to obtain the composition, wherein the contents of the components in percentage by weight are as follows: The immunity enhancement is the enhancement of the capability of resisting the infection of the edwardsiella and the aeromonas salmonicida, reduces the bacterial quantity of liver or intestinal tissues of turbot after the infection of the edwardsiella and the aeromonas salmonicida, relieves the liver injury of the turbot after the infection of the edwardsiella and the aeromonas salmonicida, and relieves the intestinal tissue injury of the turbot after the infection of the edwardsiella and the aeromonas salmonicida; The immune enhancement is to improve the expression of turbot pro-inflammatory related genes after being infected by fish killing Edwardsiella and salmonicida aeromonas, wherein the pro-inflammatory related genes comprise il1b, il6 and tnf, improve the lysozyme activity of turbot tissues, improve the sterilizing capability of turbots and improve the lysozyme activity of livers or intestinal tracts.

Description

Turbot composite immunopotentiator, and preparation method and application thereof Technical Field The invention belongs to the technical field of aquaculture feed additives, and particularly relates to a turbot compound immunopotentiator, a preparation method and application thereof. Background With the increasing exhaustion of natural fishery resources, aquaculture industry is a traditional industry, has developed rapidly and effectively in recent times, and has highlighted its importance in society, economy and people's life. However, increasing the yield from increasing the cultivation area is difficult to continuously develop due to the limited water and soil resources. Therefore, the large-scale, intensive and high-density development mode gradually becomes the main development flow of the Chinese fish farming industry. However, with the continuous steady development of fishery cultivation, various disease problems are increasingly prominent, and the cultivation yield and growth are seriously affected. Disease problems have become an important factor limiting the healthy development of the mariculture industry. For the occurrence of various diseases, chemotherapy typified by antibiotics has been positively effective in controlling and preventing diseases. However, the adverse effects of environmental pollution, the occurrence of a large number of drug-resistant pathogens, drug residues in aquatic products and the like caused by such disease control measures are becoming serious. In order to suppress the increasingly serious development trend of the cultured fish diseases caused by various environmental factors, the rapid increase of the culture density and the like, the sustainable development of the mariculture industry is promoted, and the successful experience of the development of the fishery in developed countries is advocated by the world grain and agriculture organization, and the 'system management approach' (SYSTEM MANAGEMENT approaches, SMA) culture mode for preventing the occurrence of various diseases. Improving the immunity of fish is also a way to respond to this initiative. Fish such as turbot (Scophthalmus maximus) are one of the supporting industries for marine fish farming. Along with the continuous expansion of the cultivation scale, the intensification degree is continuously improved, the disease problem in the cultivation process is also increased year by year, and especially the occurrence rate of bacterial diseases such as Edwardsiella, vibrio anguillarum and the like is highest. Meanwhile, in recent years, the improper addition of antibiotics leads to continuous improvement of drug resistance of bacteria, and serious harm is caused to green and healthy development of industry. In order to improve the immunity of aquatic animals and prevent disease, the use of an immunopotentiator becomes an important prevention and control strategy in the current aquaculture industry. Disclosure of Invention The invention aims to provide a turbot composite immunopotentiator, a preparation method and application thereof. In a first aspect of the invention there is provided the use of a composition for the preparation of an immunopotentiator for turbot, wherein the composition comprises the following components: the composition comprises the following components in percentage by weight: preferably, the composition further comprises: 1-5% of phytase. In one or more embodiments, the immune enhancement is an enhancement of the ability to combat Edwardsiella fish and/or Aeromonas salmonicida infection. In one or more embodiments, the immunopotentiator increases expression of turbot pro-inflammatory-related genes, preferably including, but not limited to, il1b, il6, tnf. In one or more embodiments, the bacterial count of turbot body tissue is reduced, preferably the bacterial count of the liver or intestinal tract is reduced. In one or more embodiments, the lysozyme activity of turbot tissue is increased, the sterilizing capacity of turbot is increased, and preferably, the lysozyme activity of liver or intestinal tract is increased. In one or more embodiments, the catalase activity of turbot tissue is increased, the antioxidant stress capacity of turbot is enhanced, and preferably, the catalase activity of liver or intestinal tract is increased. In one or more embodiments, liver damage of turbot after bacterial infection is alleviated. In one or more embodiments, the tissue damage of turbot after bacterial infection is alleviated, preferably the tissue damage comprises intestinal tissue damage. In one or more embodiments, the composition includes, in weight percent: preferably, the composition further comprises: 1.5-4% of phytase. In one or more embodiments, the turbot is an edwardsiella fish-killing (EDWARDSIELLA PISCICIDA) susceptible fish or an aeromonas salmonicida (Aeromonas salmonicidas) susceptible fish. In another aspect of the present invention, there is provided a composition which is a turb