CN-114651171-B - Automatic analysis device

CN114651171BCN 114651171 BCN114651171 BCN 114651171BCN-114651171-B

Abstract

The invention aims to provide an automatic analysis device, which can obtain stable light quantity in a wide band by combining a plurality of LED lights and can make the temperature characteristics of LED elements uniform. Referring to fig. 3, the automatic analyzer of the present invention is configured such that the light emitted from the second LED is reflected, and thus the light is combined with the light emitted from the first LED on the same optical axis, and the first LED and the second LED are in contact with the same temperature adjusting member.

Inventors

Ando Kiyo
ADACHI SAKUICHIRO
Akita Kanghong
Matsuoka Hiroshiya
Sen Hongchong
IMAMURA SHIN
Takata Eiichiro
ARAI TAKASHI

Assignees

株式会社日立高新技术

Dates

Publication Date: 20260508
Application Date: 20201007
Priority Date: 20191120

Claims (7)

1. An automatic analyzer for measuring a sample, comprising: A light source for irradiating the reaction vessel containing the sample with light, and A temperature adjusting mechanism for adjusting the temperature of the light source, The light source has a first LED and a second LED, The light source is configured to change an optical path by reflecting light emitted from the second LED on a reflecting element, thereby combining the light emitted from the first LED on the same optical axis, The temperature adjusting mechanism is composed of the same component in contact with the first LED and the second LED respectively, The second LED has a greater amount of light than the first LED, The light source further includes a first optical element that passes at least a part of the first light emitted from the first LED, and a second optical element that reflects the second light emitted from the second LED, The first optical element is configured to reflect at least a portion of the second light, The first optical element and the second optical element are arranged such that the first light having passed through the first optical element and the second light reflected by the first optical element are combined on the same optical axis to become combined light, The first LED is a white light LED generating white light with a wavelength ranging from 350nm to 800nm, The second LED is an ultraviolet LED generating ultraviolet light with a center wavelength of 350nm or less.
2. The automatic analyzer according to claim 1, wherein, The temperature adjustment mechanism is composed of 1 LED mounting substrate on which the first LED and the second LED are mounted respectively, and a temperature adjustment section that suppresses a temperature difference between the first LED and the second LED within a predetermined range by adjusting the temperature of the LED mounting substrate.
3. The automatic analyzer according to claim 1, wherein, The automatic analyzer further includes a diffusion member that diffuses the second light.
4. The automatic analyzer according to claim 3, wherein, The first light is diffused to a first diffusion range on a light receiving surface of a light receiver for receiving the combined light, The second light is diffused on the light receiving surface to a second diffusion range, The diffusion member diffuses the second light such that the second diffusion range includes the first diffusion range.
5. The automatic analyzer according to claim 1, wherein, The first optical element is configured to transmit the first light such that the combined light has a first light amount, a second light amount, a third light amount, When the first wavelength component has a fourth light amount and the second wavelength component has a fifth light amount and the third wavelength component has a sixth light amount in a wavelength spectrum of the light emitted from the halogen lamp, the first optical element is configured such that a difference between a first ratio of the second light amount to the first light amount and a second ratio of the fifth light amount to the fourth light amount falls within an allowable range, and a difference between a third ratio of the third light amount to the second light amount and a fourth ratio of the sixth light amount to the fifth light amount falls within the allowable range.
6. The automatic analyzer according to claim 1, wherein, The automatic analyzer further includes a control unit that adjusts the amount of light emitted from the light source by adjusting the driving current of the light source and the temperature of the temperature adjustment mechanism.
7. The automatic analyzer according to claim 6, wherein, The control unit obtains a temperature change with time of an environment in which the automatic analyzer is installed, The control unit sets a target temperature of the temperature adjustment mechanism according to the temperature change with time.

Description

Automatic analysis device Technical Field The present invention relates to an automatic analyzer for analyzing the amount of components contained in a sample. Background In an automatic analyzer for analyzing the component amounts of proteins, sugars, lipids, enzymes, hormones, inorganic ions, disease markers, and the like contained in biological samples such as blood and urine, a sample and a reagent are dispensed into a liquid container, and an examination item is analyzed based on a change in optical characteristics such as light absorption, fluorescence, and luminescence. In absorbance analysis in an automatic analyzer, light from a light source is irradiated to a sample or a reaction solution in which the sample and a reagent are mixed, the amount of transmitted light passing through one or more measurement wavelengths of the sample or the reaction solution is measured by a light receiving element, the absorbance is calculated, and the component amount is obtained from the relationship between the absorbance and the concentration. The light source for absorbance analysis is preferably a light source that has a broad emission spectrum for coping with a plurality of inspection items and that stably obtains a light amount equal to or more than a predetermined value at a measurement wavelength in order to measure absorbance with high accuracy. Therefore, a xenon lamp, a halogen lamp, or the like is currently used. These light sources can obtain a light quantity of a certain or more, and the time until the light quantity is stabilized is about 30 minutes or so. In addition, the light quantity is large, and accordingly, the energy consumption is large, and the lifetime is limited, and for example, in the case of a halogen lamp, replacement is required for about 1,000 hours, and the maintenance frequency as an automatic analyzer is high. In recent years, as a light source for absorbance analysis, a light emitting Diode (LIGHT EMITTING Diode, hereinafter referred to as LED) which can be expected to have a long life has been studied. For example, patent document 1 describes a structure in which LED light of halogen lamp light and ultraviolet light is combined through a filter. Particularly, the light quantity of the halogen lamp is remarkably reduced in the case of ultraviolet light, and thus an LED for ultraviolet light is used in this document. The document also attempts to monitor degradation of light quantity by using light partially reflected by a filter at the time of combining LED light of halogen lamp light and ultraviolet light, thereby maintaining high-precision analysis performance. When an LED is used as a light source for absorbance analysis, there is a concern that the analysis accuracy is lowered due to changes in the light emission spectrum and the amount of light caused by self-heating at the time of lighting or the ambient temperature. To prevent this, patent document 2 discloses a temperature control block in which an LED photometry section is in contact with a reaction cell (a member for storing a sample or a reaction solution). This document uses LEDs to achieve a compact device, and fixes the light emitting elements of the LEDs to a member having a large heat capacity to adjust the preheating temperature. Thus, the LED element is maintained at a temperature within a certain range without being affected by the external air temperature and self-heat generation, and thus light quantity stability of a certain level or more can be obtained. The automatic analyzer uses a reagent and light having different wavelengths depending on the components to be measured, and the wavelength range is a wide range of 340nm to 800 nm. Therefore, it is difficult to cover the entire band with 1 LED light, and a plurality of LEDs are used. As a method of absorbance analysis by an automatic analyzer, a 2-wavelength measurement method is known. In this method, the concentration of the measurement object is precisely quantified by measuring 2 wavelengths of light simultaneously. In this measurement method, on the premise that the optical axis of each wavelength of light coincides with the light quantity distribution with respect to the reaction solution, if these do not coincide, the original quantitative effect of the 2-wavelength measurement method with good accuracy cannot be obtained. For example, when a 2-wavelength measurement method is performed using 2-wavelength light whose optical axis and light quantity distribution are not uniform, the accuracy and precision are significantly reduced due to interference such as bubbles, as compared with the case where the light quantity distribution is uniform. Accordingly, an improvement is proposed in patent document 3 in that by providing a slit between the light source and the reaction unit, the influence of the light source image caused by the light quantity distribution is not generated. As described above, when an LED is used as a light source for absorbance analy