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CN-114798014-B - Biological particle sorting flow channel and micro-fluidic chip

CN114798014BCN 114798014 BCN114798014 BCN 114798014BCN-114798014-B

Abstract

The invention relates to a biological particle sorting flow channel and a microfluidic chip, wherein the biological particle sorting flow channel comprises a first flow channel unit, the first flow channel unit comprises a first side wall and a second side wall which are oppositely arranged, the first side wall and the second side wall are asymmetric curved surfaces, one side, close to the first side wall or the second side wall, of the first flow channel unit is dug with a deepening flow channel, and the deepening flow channel is arranged along the extending direction of the first flow channel unit. The first flow channel unit with the asymmetric curved surface is arranged to form focusing flow, the deepened flow channel is arranged along the extending direction of the first flow channel unit, the liquid flow state of the first flow channel unit near the deepened flow channel is disturbed, so that the inertial lift force and dean drag force are changed, the original balance is destroyed, particles such as white blood cells near one side of the deepened flow channel can generate disordered movement states, the overlapping of the bands of white blood cell aggregates and circulating tumor cells is avoided, and the recovery of the circulating tumor cells is facilitated.

Inventors

  • MENG XUAN
  • YANG JIAMIN

Assignees

  • 广州万孚生物技术股份有限公司

Dates

Publication Date
20260512
Application Date
20210129

Claims (9)

  1. 1. The biological particle sorting runner is characterized by comprising a first runner unit, wherein the first runner unit comprises a first side wall and a second side wall which are oppositely arranged, the first side wall and the second side wall are asymmetric curved surfaces, one side, close to the first side wall or the second side wall, of the first runner unit is provided with a deepened runner in a digging mode, and the deepened runner is arranged along the extending direction of the first runner unit; The second flow channel unit is communicated with the first flow channel unit, the curvature radius of the second flow channel unit is smaller than that of the first flow channel unit, the deepened flow channel correspondingly extends into the second flow channel unit, the second flow channel unit comprises a third side wall and a fourth side wall which are oppositely arranged, the third side wall and the fourth side wall are asymmetric curved surfaces, the third side wall is connected with the second side wall, and the fourth side wall is connected with the first side wall; the device comprises a plurality of first flow channel units and a plurality of second flow channel units, wherein the first flow channel units and the second flow channel units are alternately arranged, and the depth of the deepened flow channel is 50-200 mu m.
  2. 2. The biological particle sorting flow channel according to claim 1, further comprising a third flow channel unit in communication with the endmost second flow channel unit, the third flow channel unit having a radius of curvature greater than the radius of curvature of the first flow channel unit, the deepened flow channel extending correspondingly into the third flow channel unit, and the third flow channel unit being disposed 90 ° rotated relative to the first flow channel unit, the third flow channel unit including oppositely disposed fifth and sixth side walls having an asymmetrically curved surface, the fifth and sixth side walls being connected to an end of the third side wall remote from the second side wall, the sixth side wall being connected to an end of the fourth side wall remote from the first side wall.
  3. 3. The biological particle sorting flow channel according to claim 2, further comprising a plurality of fourth flow channel units and a plurality of fifth flow channel units, wherein the curvature radius of the fourth flow channel units is smaller than that of the fifth flow channel units, the curvature radius of the third flow channel units is larger than that of the fifth flow channel units, the fourth flow channel units and the fifth flow channel units are alternately arranged, the deepened flow channels correspondingly extend into the fourth flow channel units and the fifth flow channel units, the third flow channel units are arranged in a rotating way of 90 degrees relative to the fifth flow channel units, the fourth flow channel units comprise seventh side walls and eighth side walls which are arranged oppositely, the seventh side walls and the eighth side walls are asymmetrically curved surfaces, the fifth flow channel units comprise ninth side walls and tenth side walls which are arranged oppositely, the eighth side walls are asymmetrically curved surfaces and are connected with the ninth side walls, the seventh side walls are connected with the fifth side walls, the seventh side walls of the fourth flow channel units at the foremost end are connected with the seventh side walls and the fifth side walls of the fifth flow channel units, and the fifth flow channel units at least one end of the fifth flow channel units is connected with the eighth side walls of the fifth flow channel units, and the fifth flow channel units at least one end of the fifth flow channel units is far from the fifth side walls.
  4. 4. The biological particle sorting flow channel according to claim 3, wherein a blocking member is provided in the fifth flow channel unit at a position corresponding to the inlet of the flow dividing hole, and the blocking member is located at a side of the flow dividing hole away from the deepened flow channel, and a width of the blocking member along an extending direction of the fifth flow channel unit is larger than a diameter of the flow dividing hole.
  5. 5. The biological particle sorting flow channel according to any one of claims 3 to 4, further comprising a sixth flow channel unit communicating with the endmost fourth flow channel unit, the sixth flow channel unit having a radius of curvature greater than that of the fifth flow channel unit, the deepened flow channel extending correspondingly into the sixth flow channel unit, the sixth flow channel unit being disposed rotated 90 ° relative to the fifth flow channel unit and the sixth flow channel unit being disposed in a convex manner in an opposite direction to the third flow channel unit, the sixth flow channel unit including an eleventh side wall and a twelfth side wall disposed in an opposite manner, the eleventh side wall and the twelfth side wall being asymmetrically curved surfaces, the eleventh side wall being connected to an end of the eighth side wall remote from the ninth side wall, the twelfth side wall being connected to an end of the seventh side wall remote from the tenth side wall.
  6. 6. The biological particle sorting flow channel of claim 5, wherein the depth of the deepened flow channel is 70 μm to 120 μm.
  7. 7. The biological particle sorting flow channel of claim 5, wherein the radius of curvature of the first sidewall is greater than or less than the radius of curvature of the second sidewall, and the first sidewall and the second sidewall are disposed in a convex manner toward the same side; And/or, the curvature radius of the third side wall is smaller than that of the fourth side wall, and the third side wall and the fourth side wall are arranged in a protruding way towards the same side; And/or, the curvature radius of the fifth side wall is smaller than that of the sixth side wall, and the fifth side wall and the sixth side wall are arranged in a protruding way towards the same side; and/or, the radius of curvature of the seventh side wall is smaller than that of the eighth side wall, and the seventh side wall and the eighth side wall are arranged in a protruding way towards the same side; and/or, the radius of curvature of the ninth side wall is smaller than that of the tenth side wall, and the ninth side wall and the tenth side wall are arranged in a protruding way towards the same side; And/or, the radius of curvature of the eleventh side wall is smaller than that of the twelfth side wall, and the eleventh side wall and the twelfth side wall are arranged in a protruding mode towards the same side.
  8. 8. A microfluidic chip comprising a functional plate, wherein a first side of the functional plate is provided with the biological particle sorting channel of any one of claims 1-7.
  9. 9. The microfluidic chip is characterized by comprising a functional plate, wherein a first side surface of the functional plate is provided with the biological particle sorting flow channel according to any one of claims 3-7, a second side surface of the functional plate is provided with a buffer flow channel in a reciprocating reverse structure, and the buffer flow channel is communicated with the flow dividing hole.

Description

Biological particle sorting flow channel and micro-fluidic chip Technical Field The invention relates to the technical field of biological particle sorting, in particular to a biological particle sorting flow channel and a microfluidic chip. Background Inertial focusing microfluidics is used in the biomedical industry in cell sorting processes, such as sorting circulating tumor cells from blood. Tumor metastasis is the process by which tumor cells fall off the primary or metastatic focus and circulate in the lymphatic system or peripheral blood, thereby invading distant tissues and forming new foci, ultimately leading to death of the patient, and these fallen tumor cells are called circulating tumor cells (circulating tumor cell, CTCs). The circulating tumor cells are extremely low in content, and usually contain 1-10 circulating tumor cells in each milliliter of blood, the content of each milliliter of white blood cells is millions, the content of red blood cells is billions, and the difficulty in sorting and enriching the circulating tumor cells is similar to that of a sea fishing needle. In the traditional linear arc-shaped flow channel focusing process, circulating tumor cells and white blood cells are easy to overlap, and the sorting of the circulating tumor cells is not facilitated. Disclosure of Invention Based on the above, it is necessary to provide a biological particle sorting flow channel and a microfluidic chip, which can effectively avoid overlapping of circulating tumor cells and white blood cells, and facilitate sorting of circulating tumor cells. The utility model provides a biological particle sorting runner, includes first runner unit, first runner unit includes relative first lateral wall and the second lateral wall that sets up, first lateral wall and second lateral wall are asymmetric curved surface, dig in the first runner unit and be close to one side of first lateral wall or second lateral wall and be equipped with deepening runner, deepen the runner along first runner unit extending direction sets up. When a sample such as blood is introduced into the biological particle sorting flow channel for particle sorting, by arranging a first flow channel unit with an asymmetric curved surface, due to the stress influence of inertia lift force, dean drag force and the like, each particle such as white blood cells and circulating tumor cells in the sample can relatively move in the cross section of the first flow channel unit, when each particle moves to the balance position of the cross section, the particle can be stabilized at the position of the cross section, so that the particle is focused at the stable position in the cross section to form focused flow, and flows to the downstream, because the diameters of each particle such as white blood cells and circulating tumor cells in the blood can be gathered at different positions of the cross section of the first flow channel unit, but certain particles such as white blood cells and circulating tumor cells are closely aggregated by inertia, the deepened flow channel is formed in the first flow channel unit on one side (such as the side far away from the aggregation of the circulating tumor cells) close to the first side wall or the second side wall, and is arranged along the extending direction of the first flow channel unit, so that the liquid flowing state of the first flow channel unit close to the deepened flow channel is disturbed, the inertial lift force and the dean drag force are changed, the original balance is destroyed, particles such as white blood cells on one side of the deepened flow channel can generate disordered moving states, the overlapping of the leucocyte aggregate strips and the circulating tumor cells is avoided, the aggregation of the non-interfering circulating tumor cells is ensured, the aggregation of the white blood cells on the same side of the circulating tumor cells is also prevented, the subsequent separation of the circulating tumor cells from the white blood cells is facilitated, and the recovery of the circulating tumor cells is facilitated. In one embodiment, the bio-particle sorting flow channel further includes a second flow channel unit communicated with the first flow channel unit, the curvature radius of the second flow channel unit is smaller than that of the first flow channel unit, the deepened flow channel correspondingly extends into the second flow channel unit, the second flow channel unit includes a third side wall and a fourth side wall which are oppositely arranged, the third side wall and the fourth side wall are asymmetric curved surfaces, the third side wall is connected with the second side wall, and the fourth side wall is connected with the first side wall. In one embodiment, the bio-particle sorting flow channel includes a plurality of the first flow channel units and a plurality of the second flow channel units, and the first flow channel units and the second flow channel units are