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CN-114959096-B - SNP molecular marker related to mung bean anti-bean elephant and application thereof in genetic breeding

CN114959096BCN 114959096 BCN114959096 BCN 114959096BCN-114959096-B

Abstract

The invention discloses SNP molecular markers related to mung bean anti-elephant and application thereof in genetic breeding. The invention aims to solve the problem of how to identify the resistance of mung bean weevil. The SNP molecular marker is a locus (SNP locus) on mung bean chromosome 4, the nucleotide type of the SNP molecular marker is T or C, and the SNP molecular marker is 164 th nucleotide of a sequence 3 in a sequence table. The invention provides application of a substance for detecting polymorphism or genotype of SNP locus in mung bean genome in identification or auxiliary identification of mung bean elephant resistance. The method can be used for assisting in identifying the resistance of mung beans to bean weevils, can be used for early screening of screened mung beans, and can be used for assisting in breeding of mung bean molecular markers.

Inventors

  • CHEN HONGLIN
  • CHENG XUZHEN
  • WANG LIXIA
  • WANG SUHUA

Assignees

  • 中国农业科学院作物科学研究所

Dates

Publication Date
20260505
Application Date
20220524

Claims (9)

  1. 1. The application of the substances for detecting the polymorphism or genotype of SNP locus in mung bean genome in any one of the following, (1) Identifying or assisting in identifying green bean image resistance of green beans; (2) Screening or breeding mung bean single plants or strains or varieties resistant to the green bean weevils; (3) Screening or breeding mung bean single plants or strains or varieties of the green bean weevils; (4) Preparing a product for identifying or assisting in identifying resistance of mung beans to green bean weevils; (5) Preparing a product for screening or breeding mung bean single plants or strains or varieties resistant to green bean weevils; (6) Preparing a product for screening or breeding mung bean single plants or strains or varieties of the green bean weevils; The SNP locus is a locus on mung bean chromosome 4, the nucleotide type of the SNP locus is T or C, and the SNP locus is the 164 th nucleotide of a sequence 3 in a sequence table.
  2. 2. The method according to claim 1, wherein the substance is D1), D2) or D3): D1 The substance is a primer composition for amplifying mung bean genome DNA fragments including the SNP locus; d2 The substance is a PCR reagent containing the primer composition of D1); d3 The substance is a kit containing the primer composition of D1) or the PCR reagent of D2).
  3. 3. The method according to claim 2, wherein the primer composition comprises primer A and primer B; The primer A is a single-stranded DNA molecule with a nucleotide sequence of sequence 1 in a sequence table; The primer B is a single-stranded DNA molecule with a nucleotide sequence of sequence 2 in a sequence table.
  4. 4. The method for identifying or assisting in identifying the resistance of mung beans is characterized by comprising the steps of detecting the genotype of SNP loci in mung bean genome to be detected, identifying or assisting in identifying the resistance of mung beans according to the genotype, wherein the SNP loci are loci on mung bean chromosome 4, the nucleotide type of the SNP loci is T or C, the 164 th nucleotides of sequence 3 in a sequence table, the genotypes are TT, TC or CC, the TT is homozygous for the SNP loci, the CC is homozygous for the SNP loci, the TC is heterozygous for the SNP loci, and the TC is heterozygous for the SNP loci; The identification or auxiliary identification of the green bean image resistance of the mung beans is any one of the following modes: 1) The mung bean to be detected with the genotype of the SNP locus being TT or TC is or is candidate to be mung bean resisting green bean weevil; 2) The mung bean to be detected with the genotype of the SNP locus being CC is or is candidate to be mung bean of green bean weevil; 3) The mung bean image resistance of the mung bean to be detected with the genotype of the SNP locus being TT or TC is higher than that of the mung bean to be detected with the genotype of the SNP locus being CC, and the mung bean image resistance of the mung bean to be detected with the genotype of the SNP locus being TT is higher than that of the mung bean to be detected with the genotype of the SNP locus being TC.
  5. 5. Use of the method of claim 4 in mung bean breeding.
  6. 6. A mung bean breeding method is characterized by comprising the steps of detecting the genotype of the SNP locus in the mung bean genome according to claim 1, selecting mung beans with the genotype of the SNP locus being TT or TC as parents for breeding, wherein TT is homozygous with the SNP locus being T, TC is heterozygous with the SNP locus being T and C, mung beans with the genotype being TT or TC are mung beans with the resistance of the mung beans, and breeding the mung beans with the resistance of the mung beans.
  7. 7. A mung bean breeding method is characterized by comprising the steps of detecting the genotype of the SNP locus in claim 1 in mung bean genome, selecting mung beans with the genotype of the SNP locus as a parent for breeding, wherein CC is homozygous with the SNP locus as C, and mung beans with the genotype of CC are mung beans with mung bean image sensitivity, and the breeding purpose of the method is to breed mung beans with green bean image.
  8. The DNA molecule is characterized in that the nucleotide sequence of the DNA molecule is sequence 3 in a sequence table.
  9. 9. The use of a DNA molecule according to claim 8, (1) Identifying or assisting in identifying green bean image resistance of green beans; (2) Screening or breeding mung bean single plants or strains or varieties resistant to the green bean weevils; (3) Screening or breeding mung bean single plants or strains or varieties of the green bean weevils; (4) Preparing a product for identifying or assisting in identifying resistance of mung beans to green bean weevils; (5) Preparing a product for screening or breeding mung bean single plants or strains or varieties resistant to green bean weevils; (6) And preparing a product for screening or breeding mung bean single plants or strains or varieties of the green bean weevils.

Description

SNP molecular marker related to mung bean anti-bean elephant and application thereof in genetic breeding Technical Field The invention relates to the technical fields of molecular biology and genetic breeding, in particular to SNP molecular markers related to mung bean anti-bean weevil and application thereof in genetic breeding. Background Mung bean (VIGNA RADIATA (Linn.) Wilczek.) has a two thousand year history in China and is an important food crop in China. In recent years, along with the adjustment of the structure of the planting industry and the change of the dietary structure of people, the demand of people for mung beans is increased, and the planting area is enlarged year by year. Research shows that the plant diseases and insect pests seriously affect the germination of mung bean seeds, the yield and the like. In view of the above, the intensive research on genes and insect-resistant mechanisms involved in the anti-elephant mung bean variety has very important significance for screening dominant varieties with strong anti-elephant capability and screening anti-elephant breeding materials. The green bean weevil (Callosobruchus chinensis Linnaeus) belongs to coleoptera, the family of the bean weevil, the genus Oncomelania, is a worldwide storage pest. Legume crops rich in carbohydrates, proteins and less in fat, such as mung beans, small beans, cowpeas, broad beans, peas and chickpeas, are mainly fed, wherein mung beans are particularly preferred. The primary invasion of the green bean is generally generated in the field, the pollen is eaten by adults, the green bean can lay eggs on pods of the green bean, the green bean can be put in storage along with the beans and is continuously damaged, the secondary invasion and even multiple invasion can be caused after the emergence of the adults, and the green bean in the warehouse is even damaged and even completely scrapped when the green bean in the warehouse is serious, so that the use value is lost. In addition, the green bean weevil has strong reproductive capacity, is difficult to clear after occurrence and has serious harm. Therefore, it is necessary to select and breed the variety of the bean weevil-resistant mung bean, and the molecular marker assisted selection by using the high-flux molecular detection platform is an effective means for improving the efficiency and accuracy of breeding and selecting the disease and insect-resistant mung bean. Disclosure of Invention The invention aims to solve the problem of how to identify or assist in identifying the resistance of mung bean weevil. In order to solve the technical problems, the invention firstly provides application of a substance for detecting polymorphism or genotype of SNP loci in mung bean genome. The application provided by the invention is the application of a substance for detecting the polymorphism or genotype of SNP locus in mung bean genome in any one of the following, (1) Identifying or assisting in identifying resistance of mung bean weevil; (2) Screening or breeding mung bean single plants or strains or varieties resistant to bean weevils; (3) Screening or breeding mung bean single plants or strains or varieties of the perceived bean weevils; (4) Breeding mung beans; (5) Preparing a product for identifying or assisting in identifying the resistance of mung bean elephant; (6) Preparing a product for screening or breeding single mung bean plants or strains or varieties resistant to bean weevils; (7) Preparing a product for screening or breeding mung bean single plants or strains or varieties of the perceived bean weevils; (8) Preparing mung bean breeding products; The SNP locus is a locus on mung bean chromosome 4, the nucleotide type of the SNP locus is T or C, and the SNP locus is the 164 th nucleotide of a sequence 3 in a sequence table. The genome sequence of the middle green No. 5 is taken as a reference genome, and the SNP locus is 10060247bp of the mung bean chromosome 4 (specifically, 164 th locus of the sequence 3 in a sequence table). The invention also provides a method for identifying or assisting in identifying resistance of mung bean weevil. The method for identifying or assisting in identifying the resistance of mung bean weevils comprises the steps of detecting genotypes of SNP loci in mung bean weevils to be detected, and identifying or assisting in identifying the resistance of mung bean weevils according to the genotypes, wherein the genotypes are TT, TC or CC, the TT is homozygous for the T of the SNP loci, the CC is homozygous for the C of the SNP loci, and the TC is heterozygous for the T and the C of the SNP loci. Optionally, according to the above method, the identifying or assisting in identifying the resistance of mung bean weevil is any one of the following ways: (1) The mung bean to be detected with the genotype of the SNP locus being TT or TC is or is candidate to be the mung bean resisting the elephant, (2) The mung bean to be detected with the genotyp