CN-114989965-B - Assembly line nucleic acid extraction and library preparation instrument

CN114989965BCN 114989965 BCN114989965 BCN 114989965BCN-114989965-B

Abstract

The assembly line nucleic acid extraction and library preparation instrument comprises a frame, a vehicle running device, a nucleic acid extraction device, a library building device, a purification device and a storage device, wherein the nucleic acid extraction device, the library building device and the purification device are arranged on the frame and are positioned on one side of the vehicle running device, the storage device is matched with the nucleic acid extraction device, the library building device and the purification device through the vehicle running device, materials in the nucleic acid extraction device, the library building device, the purification device and the storage device are moved through the vehicle running device, a liquid moving device is arranged above the frame, the liquid moving device is matched with the nucleic acid extraction device and the storage device, and liquid in the storage device is moved into the nucleic acid extraction device.

Inventors

YE BAOCHUN
WANG JUN
Fang Xueyuan

Assignees

杭州杰毅生物技术有限公司
杭州杰毅生物技术有限公司

Dates

Publication Date: 20260421
Application Date: 20220615
Priority Date: 20220615

Claims (5)

1. The assembly line nucleic acid extraction and library preparation instrument is characterized by comprising a frame (1), a vehicle device (2), a nucleic acid extraction device (3), a library building device (4) and a purification device (5) which are arranged on the frame (1) and positioned on one side of the vehicle device (2), and a storage device (6) which is arranged on the frame (1) and positioned on the other side of the vehicle device (2), wherein the storage device (6) is matched with the nucleic acid extraction device (3), the library building device (4) and the purification device (5) through the vehicle device (2), materials in the nucleic acid extraction device (3), the library building device (4), the purification device (5) and the storage device (6) are moved through the vehicle device (2), a liquid moving device (7) is arranged above the frame (1), and the liquid moving device (7) is matched with the nucleic acid extraction device (3) and the storage device (6), and liquid in the storage device (6) is moved into the nucleic acid extraction device (3); The vehicle driving device (2) comprises a vehicle frame (2-1), a vehicle driving assembly (2-2), a rotating support (2-3), a transferring support (2-4) and a hooking assembly (2-5), wherein the rotating support (2-3) is slidably arranged on the vehicle frame (2-1) through a sliding rail, the vehicle driving assembly (2-2) is connected with the rotating support (2-3), the rotating support (2-3) is driven to move, the transferring support (2-4) and the hooking assembly (2-5) are respectively arranged on the rotating support (2-3), and the hooking assembly (2-5) is matched with the transferring support (2-4); The hooking assembly (2-5) comprises a hooking mounting plate (2-5-1), the hooking mounting plate (2-5-1) is slidably mounted in the rotating frame (2-3-2) through a translation piece (2-6), a lifting motor (2-5-2) is mounted on the hooking mounting plate (2-5-1), a lifting gear (2-5-3) is mounted on the lifting motor (2-5-2), a lifting rack (2-5-4) is slidably mounted on the hooking mounting plate (2-5-1), a hooking rod (2-5-5) is fixedly mounted on the lifting rack (2-5-4), and a hook (2-5-6) is formed on the hooking rod.
2. The assembly line nucleic acid extraction and library preparation instrument according to claim 1, wherein the nucleic acid extraction device (3) comprises an oscillation mechanism and a sample adding mechanism which are matched with each other, the oscillation mechanism comprises an oscillation mounting plate (3-1), an oscillation base (3-2), an oscillation test tube rack (3-3) and an oscillation magnetic attraction component (3-4), the oscillation base (3-2) is mounted on the oscillation mounting plate (3-1) through a shock pad (3-5), the oscillation mounting plate (3-1) is mounted on a frame (1), an oscillation motor (3-6) is mounted in the oscillation base (3-2), an oscillation eccentric wheel (3-7) is mounted on an output shaft of the oscillation motor (3-6), the oscillation test tube rack (3-3) is fixedly mounted above the oscillation base (3-2), and the oscillation magnetic attraction component (3-4) is mounted on the oscillation mounting plate (3-1).
3. The apparatus for extracting nucleic acid and preparing a library in a production line according to claim 1, wherein the library-creating device (4) comprises a library-creating test tube rack (4-1), a radiator (4-2) and a temperature-controlling head (4-3), the temperature-controlling head (4-3) is mounted on the radiator (4-2), the radiator (4-2) is mounted on the library-creating test tube rack, and the library-creating test tube rack (4-1) corresponds to the temperature-controlling head (4-3) one by one.
4. The nucleic acid extraction and library preparation instrument for the assembly line according to claim 1, wherein the purification device (5) comprises a purification vibration mechanism and a purification sample adding mechanism, the purification sample adding mechanism is matched with the purification vibration mechanism, the purification vibration mechanism comprises a purification base (5-1), a purification test tube rack (5-2) and a purification motor, the purification test tube rack (5-2) is fixedly arranged on the purification base (5-1), the purification motor is fixedly arranged on the purification base (5-1), an eccentric part (5-4) is arranged on a motor shaft of the purification motor, and the structures of the purification base (5-1) and the purification test tube rack (5-2) are consistent with those of the vibration base (3-2) and the vibration test tube rack (3-3).
5. The nucleic acid extraction and library preparing apparatus according to claim 1, wherein the pipetting device (7) comprises a cross beam (7-1), a pipetting lifter (7-2), an air pump (7-3) and a pipetting mount (7-4), the pipetting lifter (7-2) is mounted on the cross beam (7-1), the pipetting mount (7-4) is mounted on the pipetting lifter (7-2), a pipetting needle (7-5) is mounted on the pipetting mount (7-4), the air pump (7-3) is mounted on the pipetting mount (7-4) and is matched with the pipetting needle (7-5), and the pipetting needle (7-5) is driven to suck or discharge.

Description

Assembly line nucleic acid extraction and library preparation instrument Technical Field The invention belongs to the technical field of nucleic acid detection, and particularly relates to a pipeline nucleic acid extraction and library preparation instrument. Background Nucleic acid amplification detection techniques are widely used in various fields of life sciences such as molecular biology, medicine, law, etc., and particularly the development of high-throughput sequencing techniques plays a great role in both genetic and infectious directions. However, the preparation process of the nucleic acid library used in the high-throughput sequencing process generally comprises three steps of sample nucleic acid extraction, library construction and library purification. The automatic nucleic acid extraction and library preparation instrument disclosed at present has lower automation degree, low quality of nucleic acid and library, and easy sequencing failure, the quality of nucleic acid and library directly relates to sequencing cost, and the quality of library with poor quality can indirectly increase sequencing steps and processing time and even sequencing failure. Disclosure of Invention Aiming at the defects, the invention aims to provide a pipeline nucleic acid extraction and library preparation instrument, which realizes full-automatic nucleic acid extraction, library construction and purification, and realizes that a final product can be directly applied to downstream nucleic acid detection. In order to solve the technical problems, the invention adopts the following technical scheme: The assembly line nucleic acid extraction and library preparation instrument comprises a frame, a vehicle running device, a nucleic acid extraction device, a library building device, a purification device and a storage device, wherein the nucleic acid extraction device, the library building device and the purification device are arranged on the frame and are positioned on one side of the vehicle running device, the storage device is matched with the nucleic acid extraction device, the library building device and the purification device through the vehicle running device, materials in the nucleic acid extraction device, the library building device, the purification device and the storage device are moved through the vehicle running device, a liquid moving device is arranged above the frame, the liquid moving device is matched with the nucleic acid extraction device and the storage device, and liquid in the storage device is moved into the nucleic acid extraction device. The preferable scheme of the invention is that the vehicle running device comprises a running frame, a running driving assembly, a rotating bracket, a transferring bracket and a hooking assembly, wherein the rotating bracket is slidably arranged on the running frame through a sliding rail, the running driving assembly is connected with the rotating bracket to drive the rotating bracket to move, the transferring bracket and the hooking assembly are respectively arranged on the rotating bracket, the hooking assembly is matched with the transferring bracket, and a test tube is transferred into the transferring bracket. The hooking assembly comprises a hooking mounting plate, wherein the hooking mounting plate slides in a rotating frame through a translation piece, a lifting motor is arranged on the hooking mounting plate, a lifting gear is arranged on the lifting motor, a lifting rack is arranged on the hooking mounting plate in a sliding manner, a hooking rod is fixedly arranged on the lifting rack, and a hook is formed on the hooking rod. The nucleic acid extraction device comprises an oscillation mechanism and a sample adding mechanism which are matched with each other, wherein the oscillation mechanism comprises an oscillation mounting plate, an oscillation base, an oscillation test tube rack and an oscillation magnetic attraction component, the oscillation base is mounted on the oscillation mounting plate through a shock pad, the oscillation mounting plate is mounted on a rack, an oscillation motor is mounted in the oscillation base, an oscillation eccentric wheel is mounted on an output shaft of the oscillation motor, the oscillation test tube rack is fixedly mounted above the oscillation base, and the oscillation magnetic attraction component is mounted on the oscillation mounting plate. As a preferable scheme of the invention, the sampling mechanism comprises a sampling bracket, a sampling vertical component, a sampling longitudinal component and a sampling outlet component, wherein the sampling longitudinal component is arranged on the sampling bracket, the sampling vertical component is arranged on the sampling longitudinal component, and the sampling outlet component is arranged on the sampling vertical component and is matched with the sampling vertical component. The library building device comprises a library building test tube rack, a radiator and