CN-115491400-B - Marine biological peptide for promoting skin wound repair and preparation method and application thereof

Abstract

The invention discloses a marine biological peptide capable of effectively promoting skin wound repair and a preparation method and application thereof. The marine peptide has good skin compatibility, can obviously promote cell proliferation, migration and angiogenesis, effectively reduce inflammatory reaction, promote high-quality wound repair and reduce scar formation. The preparation method of the marine biological peptide comprises the steps of taking fresh and alive marine shellfish soft parts, carrying out enzymolysis by protease, ultrafiltration and sephadex separation, discarding the last elution single peak, respectively collecting or mixing other elution components, and freeze-drying. The method is simple, low in cost, environment-friendly, short in production period and suitable for industrial production. The marine biological peptide can be applied to medicines, medical biological materials and cosmetics, has wide application range, and has obvious effects of promoting regeneration and repair of burns, scalds, cut wounds, traffic wounds, operation wounds, war wounds, firearm wounds, seawater-soaked wounds, diabetic ulcers, inflammatory ulcers, pressure ulcers and chronic difficultly-healed wound surfaces.

Inventors

• CHEN HUA
• SUN HUILI
• PAN JIANYU
• CHEN DEKE
• CAI BINGNA
• WAN PENG

Assignees

• 中国科学院南海海洋研究所

Dates

Publication Date

20260508

Application Date

20220831

Claims (1)

1. 1. The application of the marine biological peptide in preparing medicines, medical biological materials or cosmetics for promoting the repair of the wound surface of a fire arm is characterized in that the marine biological peptide is obtained by taking fresh chlamys farreri which accords with the use standard of related marine products, taking soft part homogenate, adding distilled water with the volume of 2.5 times, adjusting the pH value to 7.0, adding neutral protease of 2000U/G shellfish meat, carrying out enzymolysis for 5 hours at the temperature of 50 ℃, boiling enzymolysis liquid for 10 minutes, centrifuging for 15 minutes at 6000 Xg, sequentially ultrafiltering supernatant by an ultrafiltration membrane with the interception molecular weight of 10kDa and 3kDa, wherein the active peptide component of 3kDa-10kDa is marked as N1, concentrating the N1 component to the concentration of about 200mg/mL, separating by dextran gel G-15 with the concentration of 100X 4.0cm, wherein the loading amount is 7mL, the mobile phase is distilled water with the flow rate of 10mL/min, and the detection wavelength of 220nm and 280nm, and collecting the elution component of 50-110 minutes in N1F2 to obtain the marine biological peptide; the preparation formulation of the medicine comprises lotion, ointment, tincture, spirit, powder, oil, paste, plaster, film coating agent or aerosol, wherein the mass fraction of the marine biological peptide in the medicine is 0.01% -2.5%; the medical biological material dosage form comprises lotion, solution, dressing, ointment or injection, and the mass fraction of the marine biological peptide in the medical biological material is 0.8% -2.5%; The cosmetic preparation comprises cream, emulsion, gel, water or spray, wherein the mass fraction of the marine organism peptide in the cosmetic is 0.05% -1.0%.

Description

Marine biological peptide for promoting skin wound repair and preparation method and application thereof Technical Field The invention belongs to the technical field of biological medicine, and particularly relates to a marine biological peptide for promoting skin wound repair, and a preparation method and application thereof. Background The skin is covered on the surface of the body, is one of the organs with the largest area of the human body, and is also the first barrier of the human body against external injury. The skin is in direct contact with the outside, and is easily damaged by the processes of wounds, burns and the like. According to statistics of world health organization, about 900 ten thousand people burn and scald in 2017. In addition to acute wounds, a variety of common diseases (e.g., diabetic foot, aging, obesity, vascular disease, cancer, infection, etc.) affect over 1% of people worldwide, making their wounds slow or incomplete to heal, and the time of wound healing is closely related to susceptibility to infection, pain duration, hospitalization time, and incidence of scarring. Skin wound repair mechanisms are often affected by numerous factors, such as age, nutritional status, endocrine changes, local blood circulation, infection, ionizing radiation, drugs, systemic diseases, and the like. The injury with small area can heal in several days without scar. However, in wounds with more tissue cell loss and overlarge wounds, the regenerated epidermis is difficult to repair the wounds completely, the organism fills the wounds by connective tissues, scars are often left after the wounds are repaired, and the surfaces of the wounds are covered by keratinized epidermis, but lack sweat glands, hair and dermal papilla, so that the original tissue structure and function are lost. In addition, the chronic wound healing capacity is also quite obvious. In diabetes, the incidence of diabetic foot is as high as 25%, abnormal hyperglycemia leads to damage of tissue cells and abnormal metabolism, and seriously impairs wound repair rate. In obese people, the thickness of skin fat layer is unfavorable for micro-angiogenesis, and the wound is easy to cause fat liquefaction to generate infection, so that the wound recovery speed is low. In the aging population, the self-renewal function of stem cells and the clearance ability of damaged cells are weakened due to the decline of cell compensatory function, and the wound healing rate is obviously slowed down. It follows that promoting rapid, high quality healing of these acute and chronic wounds is a non-negligible problem in clinical care. In clinical treatment, different treatments and administration are mainly performed for acute skin wounds and chronic skin wounds. The new medicine recombinant bovine basic fibroblast growth factor (rb-bFGF) of the genetic engineering class I of China promotes wound healing, and is mainly used for burn, chronic ulcer on the body surface, fresh wound (including trauma, skin-supply wound, operation wound and the like). However, the polypeptide and the recombinant protein cannot be applied and popularized in large scale in the fields of medical biomaterials, cosmetics and the like due to higher preparation cost. Disclosure of Invention It is an object of the present invention to provide a marine peptide which is effective in promoting skin wound repair. The marine biological peptide has good skin compatibility, can obviously improve cell activity, promote cell proliferation, migration and angiogenesis, effectively reduce inflammatory reaction, promote high-quality repair of skin wounds and effectively reduce scar hyperplasia. It is another object of the present invention to provide a method for preparing marine peptides by enzymatic hydrolysis, ultrafiltration, sephadex G-15 separation. The method is simple, low in cost, environment-friendly, short in production period and suitable for large-scale industrial production. It is a further object of the present invention to provide the use of a marine peptide which is useful in medicine, but also in medical biomaterials and cosmetics. The invention realizes the aim through the following technical scheme: In one aspect, the invention provides a marine peptide which has good water solubility and skin compatibility. The marine biological peptide is a product obtained by carrying out enzymolysis on fresh marine shellfish soft parts by protease. These marine shellfish may include, but are not limited to, one or a combination of several of Pinctada martensii, paphia undulata, scallops. The protease used may be an enzyme capable of enzymatic hydrolysis of the protein, including but not limited to one or a combination of papain, neutral protease, trypsin. The inventor obtains good effect by using the marine shellfish peptide in skin care and hair care research. However, since the skin wound tissue and the normal skin tissue have obvious differences in physiological state and structure, n