CN-115697562-B - Modular simultaneous diagnostic polymerase chain reaction system

CN115697562BCN 115697562 BCN115697562 BCN 115697562BCN-115697562-B

Abstract

The present invention relates to a modular simultaneous diagnosis polymerase chain reaction system, and more particularly, to a modular simultaneous diagnosis polymerase chain reaction system in which a plurality of placement grooves for placing biochips are formed in an upper surface of a main body case, a plurality of heating and cooling modules and a plurality of light source modules are provided in the main body case so as to correspond to the placement grooves, a plurality of cover members including detection modules corresponding to the placement grooves are provided in the upper surface of the main body case so as to be rotatable up and down, and the plurality of heating and cooling modules, the plurality of light source modules, and the plurality of detection modules are integrally controlled by one control module provided in the main body case, so that a sample accommodated in the plurality of biochips can be simultaneously subjected to polymerase chain reaction inspection, a plurality of polymerase chain reaction operations can be simplified, maintenance costs can be reduced, and a product price can be reduced by omitting a duplicate member.

Inventors

XU YOUZHEN
Jin Xiongxie
Pu Junxi
LI YUANZAI

Assignees

基因系统有限公司

Dates

Publication Date: 20260512
Application Date: 20220407
Priority Date: 20210530

Claims (4)

1. A modular simultaneous diagnostic polymerase chain reaction system comprising a main body case forming an external appearance to form an internal space of a predetermined size, a plurality of placement grooves formed in a recessed manner on an upper surface of the main body case for placing a plurality of biochips, a plurality of heating/cooling modules provided in the main body case so as to be positioned at a lower portion of the plurality of placement grooves for heating or cooling the biochips, a plurality of light source modules provided in the main body case so as to be positioned at a side of the plurality of placement grooves for irradiating light to a side of the biochips, and a plurality of cover members provided on an upper surface of the main body case so as to be rotatable up and down to cover the plurality of placement grooves, A step-shaped height difference part with a predetermined height is formed at the center of the upper surface of the main body casing, a lower upper plate is arranged at the front part and an upper plate is arranged at the rear part, a display panel is arranged at the front surface of the main body casing in a manner of being capable of rotating up and down, the display panel is used for displaying the working state of the polymerase chain reaction module, a control module is arranged in the main body casing and is used for controlling the polymerase chain reaction module, The cover member includes: a housing provided on the lower end plate and the upper end plate of the main body housing so as to be rotatable up and down, forming an internal space of a predetermined size, and having a through portion formed on a bottom surface thereof, the through portion corresponding to the placement groove; A rotating member disposed at the rear of the housing so that the housing can rotate up and down; a handle surrounding a part of the front surface and both side surfaces of the housing to vertically rotate the housing around the rotating member; A detection module provided at an upper portion of the penetration portion to measure fluorescence emitted from the biochip placed in the placement groove, and A light emitting diode display unit vertically arranged in front of the penetration unit for transmitting the operating state of the polymerase chain reaction module to the upper surface of the cover member by light, The control module is provided to be received by sliding the opening formed in the rear panel of the main body casing in the front-rear direction, and is electrically connected to the plurality of heating/cooling modules, the light source module, the detection module, the light emitting diode display unit, and the display panel to control them, The light emitting diode display part includes a light emitting diode substrate in which a plurality of light emitting diodes are horizontally arranged, a light guide plate formed of a transparent material having at least 4 reflecting surfaces to allow light emitted from the light emitting diode substrate to be transmitted to an upper portion through the plurality of reflecting surfaces, and a base to fix the light emitting diode substrate and the light guide plate to a bottom surface of the cover member by supporting them.
2. The modular simultaneous diagnostic polymerase chain reaction system of claim 1, wherein an opening of a predetermined size is formed in the front surface of the main body case so that the link member rotatably supporting the display panel can be moved into and out of the main body case.
3. The modular simultaneous diagnostic polymerase chain reaction system according to claim 1, wherein a plurality of openings are formed in the upper plate of the main body case so as to correspond to the plurality of placing grooves, a placing plate having a through hole formed in the center is provided in the lower portion of the opening so as to form the placing groove, a support plate for supporting the heating and cooling module is fixedly provided in the lower portion of the placing plate, the rotating member for supporting the cover member so as to be rotatable up and down is vertically provided in the upper surface of the rear end of the support plate, horizontal fixing shafts for fixing the cover member are provided in the upper surfaces of the left and right sides of the support plate, and the light source module is provided in the placing plate so as to be located in the side of the placing groove.
4. The modular simultaneous diagnostic polymerase chain reaction system according to claim 3, wherein a center groove is formed in an inner surface of both ends of the handle, a rotation shaft protruding from an outer surface of the cover member is inserted into the center groove, the handle is rotatably provided centering on the center groove, a guide groove is formed so that a horizontal fixing shaft provided on an upper surface of the support plate and protruding toward an upper surface of the main body case is inserted and moved, the guide groove is opened downward when the handle is rotated upward and erected vertically, and a locking function is provided to fix the horizontal fixing shaft so as not to be separated when the handle is rotated downward and laid horizontally.

Description

Modular simultaneous diagnostic polymerase chain reaction system Cross reference The present application claims priority from korean patent application No. 10-2021-0069619 filed 5/30 in 2021, the entire contents of which are incorporated herein by reference. Technical Field The present invention relates to a Polymerase Chain Reaction (PCR) apparatus, and more particularly, to a modular simultaneous diagnosis polymerase chain reaction system in which a plurality of polymerase chain reaction modules are provided in one apparatus and can be controlled by one control module. Background The polymerase chain reaction (Polymerase Chain Reaction; PCR) is a technique in which a sample solution containing a nucleic acid molecule is repeatedly heated and cooled and a site having a specific base sequence (target nucleic acid) is chain-copied to be amplified exponentially, and a technique in which a target gene can be detected by copying a minute amount of nucleic acid. This polymerase chain reaction method repeats a deoxyribonucleic acid (DNA) denaturation step, an annealing step, and a deoxyribonucleic acid synthesis step, the deoxyribonucleic acid denaturation step (denaturing step) being a step of heating a sample solution containing double-stranded template deoxyribonucleic acid at a specific temperature, for example, about 95 ℃, for 5 seconds to separate double-stranded deoxyribonucleic acid into single-stranded deoxyribonucleic acid, the annealing step (ANNEALING STEP) being a step of injecting a primer having a sequence complementary to a specific base sequence to be amplified into the sample solution after the deoxyribonucleic acid denaturation step, cooling for 5 seconds at a specific temperature, for example, 50 ℃, together with the single-stranded deoxyribonucleic acid, so that the primer hybridizes to the specific base sequence of the single-stranded deoxyribonucleic acid to form a partial deoxyribonucleic acid-primer complex, and the deoxyribonucleic acid synthesis step (extension step) being a step of holding the sample solution at an activation temperature of a deoxyribonucleic acid polymerase, for example, 72 ℃, for 5 seconds, after the annealing step, so that a double-stranded ribonucleic acid is formed by the deoxyribonucleic acid polymerase (ras) with the deoxidizing primer of a partial deoxyribonucleic acid-primer complex. In addition, the target nucleic acid can be detected by detecting fluorescence generated by a fluorescent dye that binds to the target nucleic acid. FIG. 1 is a schematic configuration diagram of a conventional polymerase chain reaction apparatus, and FIG. 2 is a view showing the state of use of the polymerase chain reaction apparatus shown in FIG. 1. As shown in the drawing, the conventional polymerase chain reaction apparatus 100 mainly includes a main body housing 110, a polymerase chain reaction module 120, a control module 130, and a biochip 140. The main body case 110 has a hexahedral shape and forms a space of a predetermined size inside, and forms the outer appearance of the polymerase chain reaction apparatus 100. The polymerase chain reaction module 120 heats and cools the reactant stored in the biochip 140, and is provided in a rectangular parallelepiped shape so as to be stored in the main body case 110 by sliding an opening formed in the front surface of the main body case 110 into the main body case 110. The control module 130 is disposed inside the main body case 110, electrically connected to the polymerase chain reaction module 120, and performs a predetermined polymerase chain reaction process by controlling the polymerase chain reaction module 120. The biochip 140 has a plurality of chambers for accommodating reagents including a sample and a reagent, and is placed in a placement groove 122 formed in the upper surface of the polymerase chain reaction module 120 and is provided in the main body case 110 together with the polymerase chain reaction module 120. The polymerase chain reaction module 120 includes a heating/cooling module 150 for heating and cooling the biochip 140 placed in the placement groove 122, and a light source module 160 for applying light of a predetermined wavelength to a side surface of the biochip 140. The heating and cooling module 150 includes a heating plate 151, a heat dissipating block 153, and a heat dissipating fan 155, and the light source module 160 includes a plurality of Light Emitting Diodes (LEDs) 161 and a filter 163. Also, a detection module 170 is provided at an upper portion of the placement groove 122 inside the main body case 110 to detect fluorescence emitted from the biochip 140 placed in the placement groove 122 of the polymerase chain reaction module 120. The detection module 170 includes an image sensor 171 and a filter 173. A display panel 180 is provided on the upper surface of the main body case 110 so as to be rotatable up and down. The control module 130 includes a control unit 131 and a power supply unit 133. U