CN-115867627-B - Gasket assembly and related systems and methods

Abstract

Gasket assemblies and related systems and methods are disclosed. An apparatus includes a system, a flow cell, and a plurality of gasket assemblies. The system includes a flow cell interface and the flow cell has one or more channels. Each channel has a first channel opening and a second channel opening. The first channel opening is positioned at a first end of the flow cell and the second channel opening is positioned at a second end of the flow cell. A gasket assembly is coupled at each second channel opening. Each gasket assembly includes an adhesive stack and a gasket. The adhesive stack includes a first side bonded to the gasket and a second side bonded to the flow cell. The flow cell interface may engage with a corresponding gasket to establish a fluid coupling between the system and the flow cell.

Inventors

• A. KUMAR
• J. osmos
• D. KAPLAN
• R. LeMoyne

Assignees

• 伊鲁米纳公司

Dates

Publication Date

20260508

Application Date

20220120

Priority Date

20210405

Claims (20)

1. 1. An apparatus, the apparatus comprising: A flow-through cell comprising means having a cover extending over a reaction structure to form one or more channels therebetween, each channel being in communication with a plurality of reaction sites of the reaction structure, wherein the flow-through cell has one or more channels, each channel having a first channel opening and a second channel opening, the first channel opening being positioned at a first end of the flow-through cell and the second channel opening being positioned at a second end of the flow-through cell, and A gasket assembly coupled at each second channel opening and configured to establish a fluid coupling between the flow cell and a system external to the flow cell, each gasket assembly comprising: Adhesive laminate, and Wherein the adhesive stack comprises a first adhesive coupled to the flow cell and a second adhesive coupled to the gasket and positioned between the first adhesive and the gasket, wherein each gasket assembly further comprises a release layer positioned between the first adhesive and the second adhesive, the first adhesive bonded to both the flow cell and the release layer and the second adhesive bonded to both the release layer and the gasket.
2. 2. The apparatus of claim 1, wherein the adhesive stack has a through hole and the gasket has a through hole, the through hole of the gasket aligned with the through hole of the adhesive stack to enable fluid communication through the gasket assembly.
3. 3. The apparatus of claim 1, wherein the separation layer comprises polyethylene terephthalate.
4. 4. The apparatus of claim 1, wherein the separation layer comprises a through hole and the gasket has a through hole aligned with the through hole of the separation layer, and wherein the first adhesive coats a first side of the separation layer and the second adhesive coats a second side of the separation layer.
5. 5. The apparatus of claim 1, wherein the first adhesive comprises an acrylic adhesive.
6. 6. The apparatus of claim 1, wherein the second adhesive comprises a silicone adhesive.
7. 7. The apparatus of any one of claims 1-2, wherein the gasket comprises a silicone elastomer.
8. 8. The apparatus of any one of claims 1-2, further comprising a flow cell manifold, the flow-through Chi Qiguan being coupled to the first end of the flow cell and comprising a flow-through Chi Qiguan inlet, a plurality of fluid lines, and a plurality of flow cell manifold outlets fluidly coupled to the flow cell manifold inlet by corresponding fluid lines, each of the flow cell manifold outlets being coupled to a corresponding first channel opening of the flow cell.
9. 9. The apparatus of claim 8, further comprising a manifold gasket assembly coupled to the flow cell manifold inlet.
10. 10. The apparatus of claim 9, wherein the manifold gasket assembly comprises a first adhesive coupled to the flow-through Chi Qiguan, a gasket, and a second adhesive coupled to the gasket and positioned between the first adhesive and the gasket.
11. 11. The apparatus of claim 8, wherein the flow cell manifold comprises a laminate.
12. 12. The apparatus of any one of claims 1 to 2, wherein the flow cell comprises a plurality of the channels.
13. 13. An apparatus, comprising: A system comprising a flow-through Chi Jiekou; A flow-through cell comprising means having a cover extending over a reaction structure to form one or more channels therebetween, each channel being in communication with a plurality of reaction sites of the reaction structure, wherein each channel has a first channel opening and a second channel opening, the first channel opening being positioned at a first end of the flow-through cell and the second channel opening being positioned at a second end of the flow-through cell, and A gasket assembly coupled at each second channel opening, each gasket assembly comprising: Adhesive laminate, and A gasket, the adhesive stack comprising a first side bonded to the gasket and a second side bonded to the flow cell, wherein the adhesive stack comprises a first adhesive coupled to the flow cell and a second adhesive coupled to the gasket and positioned between the first adhesive and the gasket, wherein each gasket assembly further comprises a separation layer positioned between the first adhesive and the second adhesive, the first adhesive bonded to both the flow cell and the separation layer and the second adhesive bonded to both the separation layer and the gasket; Wherein the flow cell interface is engageable with a corresponding gasket to establish a fluid coupling between a system and the flow cell.
14. 14. The apparatus of claim 13, wherein the flow cell interface comprises a plurality of plungers engageable with the corresponding gaskets.
15. 15. The apparatus of claim 14, further comprising a spring biasing the corresponding plunger.
16. 16. The apparatus of any one of claims 14 to 15, wherein the flow cell interface comprises a plunger guide comprising a plunger bore in which the corresponding plunger is positioned.
17. 17. The apparatus of any one of claims 13 to 15, wherein the system further comprises a vacuum chuck supporting the flow cell.
18. 18. The apparatus of claim 17, wherein the vacuum chuck supports a substantial length of the flow cell between the first end and the second end.
19. 19. The apparatus of any one of claims 13 to 15, further comprising a flow cell frame, the flow cell and plurality of gasket assemblies coupled to the flow cell frame.
20. 20. A method of manufacturing the apparatus of claim 1, the method comprising: picking up a gasket assembly using a head of a pick-and-place machine, the gasket assembly comprising an adhesive stack and a gasket, the adhesive stack comprising a second side and a first side bonded to the gasket; wherein the adhesive stack comprises a first adhesive on the first side of the adhesive stack, a second adhesive on the second side of the adhesive stack, and a separating layer positioned between the first adhesive and the second adhesive, and The second side of the gasket assembly is placed onto a surface surrounding the opening of the channel of the flow cell.

Description

Gasket assembly and related systems and methods Related patent application The present application claims the benefit and priority of U.S. provisional patent application No. 63/170,946 filed on 5 th 4 th year 2021 and U.S. provisional patent application No. 63/199,916 filed on 2 nd year 2021, the contents of each of which are incorporated herein by reference in their entirety for all purposes. Background The sequencing platform may include a fluidic interface that may form a fluidic connection with the flow cell. Disclosure of Invention By providing a gasket assembly and related systems and methods, advantages over the prior art as well as the benefits described later in this disclosure may be realized. Various implementations of the apparatus and methods are described below, and these apparatus and methods (including and excluding additional implementations listed below) can overcome these disadvantages and achieve the benefits described herein in any combination, provided that the combinations are not inconsistent. According to a first implementation, an apparatus includes a flow cell. The flow cell has one or more channels. Each channel has a first channel opening and a second channel opening. The first channel opening is positioned at a first end of the flow cell and the second channel opening is positioned at a second end of the flow cell. A gasket assembly is coupled at each second channel opening. Each gasket assembly includes an adhesive stack and a gasket. The adhesive stack includes a first side bonded to the gasket and a second side bonded to the flow cell. According to a second implementation, an apparatus includes a gasket assembly including a gasket and an adhesive stack including a first adhesive, a release layer, and a second adhesive. The release layer has a second side and a first side at least partially covered by a first adhesive. The second adhesive at least partially covers the second side of the separation layer. The release layer is positioned between the first adhesive and the second adhesive. The gasket is bonded to the second adhesive. The second adhesive is positioned between the release layer and the gasket. The apparatus also includes a release liner to which the first adhesive of the adhesive stack is releasably adhered. According to a third implementation, an apparatus includes a system and a flow cell. The system includes a flow cell interface and the flow cell has one or more channels. Each channel has a first channel opening and a second channel opening. The first channel opening is positioned at a first end of the flow cell and the second channel opening is positioned at a second end of the flow cell. A gasket assembly is coupled at each second channel opening. Each gasket assembly includes an adhesive stack and a gasket. The adhesive stack includes a first side bonded to the gasket and a second side bonded to the flow cell. The flow cell interface may engage with a corresponding gasket to establish a fluid coupling between the system and the flow cell. According to a fourth implementation, a method includes picking up a gasket assembly using a head of a pick-and-place machine. The gasket assembly includes an adhesive stack and a gasket. The adhesive stack includes a second side and a first side bonded to the gasket. The method includes placing a second side of the gasket assembly onto a surface surrounding an opening of a channel of the flow cell. According to a fifth implementation, an apparatus includes a flow cell having a channel including a channel opening and a gasket assembly coupled at the channel opening. The gasket assembly includes an adhesive stack and a gasket. The adhesive stack includes a first side bonded to the gasket and a second side bonded to the flow cell. According to a sixth implementation, an apparatus includes an adhesive-backed gasket. According to a seventh implementation, a method includes picking up an adhesive-backed gasket and placing the adhesive-backed gasket on a flow cell. The method further includes pressing the adhesive-backed gasket to the flow cell, thereby coupling the adhesive-backed gasket to the flow cell. Further in accordance with the foregoing first, second, third, fourth, fifth, sixth, and/or seventh implementations, the apparatus and/or method may further comprise any one or more of: In one implementation, the adhesive stack has a through hole and the gasket has a through hole that aligns with the through hole of the adhesive stack to enable fluid communication through the gasket assembly. In another implementation, the adhesive stack includes a first adhesive coupled to the flow cell and a second adhesive coupled to the gasket and positioned between the first adhesive and the gasket. In another implementation, each gasket assembly further includes a separation layer positioned between the first adhesive and the second adhesive. The first adhesive bonds to both the flow cell and the separation layer and the second adhesive