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CN-115873740-B - Thiobacillus caldus suitable for Sb leaching

CN115873740BCN 115873740 BCN115873740 BCN 115873740BCN-115873740-B

Abstract

The invention relates to the field of environmental protection, and discloses thiobacillus caldus (Acidithiobacilluscaldus) suitable for Sb leaching, wherein the preservation number of the thiobacillus caldus is CCTCC No. M2021710. The thiobacillus caldus (Acidithiobacilluscaldus) disclosed by the invention can resist a high-dose FCC spent catalyst, has a remarkable effect of removing metals (especially Sb) in the FCC spent catalyst, and is suitable for bioleaching treatment of the FCC spent catalyst.

Inventors

  • WANG YUEJIE
  • WANG CHEN
  • LI LINGLING
  • ZHAO SHENG

Assignees

  • 中国石油化工股份有限公司
  • 中石化安全工程研究院有限公司

Dates

Publication Date
20260512
Application Date
20210929

Claims (13)

  1. 1. A strain of thiobacillus caldus (Acidithiobacillus caldus) is characterized in that the preservation number of the thiobacillus caldus is CCTCC No. M2021710.
  2. 2. A microbial inoculum comprising the thiobacillus caldus (Acidithiobacillus caldus) as set forth in claim 1.
  3. 3. The microbial agent of claim 2, wherein the microbial agent is a liquid microbial agent.
  4. 4. Use of the thiobacillus caldus of claim 1 and/or the microbial inoculum of claim 2 or 3 in biological metallurgy.
  5. 5. Use of the thiobacillus caldus of claim 1 and/or the microbial inoculum of claim 2 or 3 for bioleaching metals from waste.
  6. 6. A method for removing metals from waste by bioleaching, which is characterized in that the method comprises the step of contacting the thiobacillus caldus of claim 1 and/or the microbial inoculum of claim 2 or 3 with the waste.
  7. 7. The method according to claim 6, wherein the thiobacillus caldus is contacted with waste in the form of a microbial inoculum, and the microbial inoculum is prepared by inoculating the thiobacillus caldus into a bioleaching medium for propagation to obtain a seed solution, and then inoculating the seed solution into the bioleaching medium for fermentation to obtain the microbial inoculum.
  8. 8. The method of claim 7, wherein the bioleaching medium comprises ：(NH 4 ) 2 SO 4 2-3g/L,KH 2 PO 4 2-4g/L,MgSO 4 ·7H 2 O 0.3-0.7g/L,CaCl 2 0.2-0.3g/L,FeSO 4 0.005-0.02g/L, and 8-15g/L sulfur powder, and the pH of the bioleaching medium is 2.5-3.5.
  9. 9. The method according to claim 7, wherein the conditions for the propagation comprise a temperature of 40-45 ℃ for 48-96 hours; the fermentation conditions comprise 40-45 ℃ and 24-120h, and ventilation of 0.05-0.2vvm.
  10. 10. The method according to claim 7, wherein the viable count of the thiobacillus caldus in the microbial inoculum is 10 8 cfu/mL or more.
  11. 11. The method of claim 6 or 7, wherein the waste is FCC spent catalyst.
  12. 12. The method of claim 11, wherein the FCC spent catalyst is added in an amount of 150-200g compared to 1L of the fermentation broth.
  13. 13. The method of claim 12, wherein the contacting conditions comprise a temperature of 40-45 ℃ for a period of 5-7 days.

Description

Thiobacillus caldus suitable for Sb leaching Technical Field The invention relates to the field of environmental protection, in particular to a thiobacillus caldus (Acidithiobacilluscaldus) and a microbial inoculum, and application thereof in biological metallurgy and biological leaching for removing metals in wastes and a method for removing metals in wastes by biological leaching. Background Catalytic fluid cracking (FCC) catalysts are currently the most consumed refinery catalysts and may represent 70% of the total refinery catalyst usage. During the use process of the FCC catalyst, the catalyst can lose activity due to deposition of metal components such as nickel, vanadium and the like in coke and raw oil, and the catalyst can not be used continuously, so that the catalyst becomes a waste catalyst. Since the FCC spent catalyst contains metal components having high risk such as Ni, V, sb, etc., it has been considered to have high environmental risks. In view of the harmfulness of the FCC spent catalyst, it is extremely important to dispose of it by demetallization innocent treatment. The methods for disposing the waste catalyst and recovering the noble metal which are widely adopted at present mainly comprise two processes of pyrometallurgy and hydrometallurgy, but the two treatment methods have the problems of high energy consumption, serious secondary pollution and the like. Bioleaching techniques are based on the metabolic activity of various bacteria for the purpose of metal removal. Compared with the traditional wet process and fire process, the bioleaching method has the advantages of environmental protection, low cost, simple operation and maintenance, mild required treatment conditions, no emission of dangerous waste and the like. However, the current attention and research on treating the FCC spent catalyst by a bioleaching method are very limited, the leaching efficiency of metal is low, and the popularization and application of the technology are greatly limited. And the FCC dead catalyst is rich in various metal components with higher toxicity, so that the existing bioleaching strain has poor tolerance to the FCC dead catalyst with higher concentration, generally only has a removal effect on 1% -5% of the dead catalyst, and greatly limits the application of bioleaching technology. It is desirable to increase the bioleaching efficiency of FCC spent catalysts by screening for high tolerance and high removal rate microorganisms. Disclosure of Invention The invention aims to provide a thiobacillus caldus (Acidithiobacilluscaldus) and a microbial inoculum, and application thereof in biological metallurgy and biological leaching for removing metals in wastes and a method for removing metals in wastes by biological leaching, the strain can tolerate a high dosage of FCC spent catalyst, is obviously higher than the adding amount of the prior strain of 1-5% of the spent catalyst, and has obvious effect of removing metals in the FCC spent catalyst with the adding amount of 20% by weight. In order to achieve the above purpose, the invention provides a thiobacillus caldus (Acidithiobacilluscaldus) with a preservation number of CCTCC No. M2021710. In a second aspect, the invention provides a microbial agent comprising thiobacillus caldus (Acidithiobacilluscaldus) as described above. In a third aspect the invention provides the use of a thiobacillus caldus as described above and/or a microbial agent as described above in biological metallurgy. In a fourth aspect, the invention provides the use of a thiobacillus caldus as described above and/or a microbial inoculum as described above for bioleaching metals from waste. In a fifth aspect the invention provides a method of bioleaching to remove metal from waste, the method comprising contacting a thiobacillus caldus as described above and/or a microbial inoculum as described above with the waste. The thiobacillus caldus provided by the invention can tolerate high dosage (up to 40 wt%) of FCC spent catalyst, is obviously higher than the adding amount of 1% -5% of the spent catalyst of the existing strain, and has obvious effect of removing metals in 20 wt% of the added FCC spent catalyst. The invention provides a usable strain for removing metals (especially Sb) in the FCC spent catalyst by a bioleaching method, and is expected to realize the industrial application of treating the FCC spent catalyst by the bioleaching method. Additional features and advantages of the invention will be set forth in the detailed description which follows. Preservation of organisms The strain of the invention is thiobacillus caldus (Acidithiobacillus caldus), which is preserved in China center for type culture Collection (address: wuhan university, postal code: 430072) (abbreviated as CCTCC of preservation unit) at 6 and 9 days of 2021, with a preservation number of CCTCC No. M2021710. Detailed Description The endpoints and any values of the ranges disclosed herein are not limited