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CN-115980338-B - Array type food allergen detection micro-fluidic chip, clamp and detection system

CN115980338BCN 115980338 BCN115980338 BCN 115980338BCN-115980338-B

Abstract

The invention provides an array type food allergen detection microfluidic chip, a clamp and a detection system. The chip comprises a first microfluidic chip and a second microfluidic chip which share a substrate, wherein the first microfluidic chip comprises a first runner group arranged on the substrate, the second microfluidic chip comprises a second runner group arranged on the substrate, the first runner group comprises at least two longitudinal runners which are arranged in parallel, one longitudinal runner is used for blank comparison, the other longitudinal runners are used for modifying target antibodies, the second runner group comprises at least two transverse runners which are arranged in parallel, the transverse runners are used for loading samples and fluorescent marks, the transverse runners are vertically attached to modification marks of the longitudinal runners, and an array point is formed at the intersection of the transverse runners and the modification marks of the longitudinal runners.

Inventors

  • LI ZHENG
  • CHEN XIAOFENG

Assignees

  • 深圳大学

Dates

Publication Date
20260505
Application Date
20221220

Claims (10)

  1. 1. An array type food allergen detection micro-fluidic chip is characterized by comprising a first micro-fluidic chip and a second micro-fluidic chip which share a substrate, wherein the first micro-fluidic chip comprises a first flow channel group (110) arranged on the substrate, and the second micro-fluidic chip comprises a second flow channel group (120) arranged on the substrate; the first flow channel group (110) comprises at least two parallel longitudinal flow channels (111), wherein one longitudinal flow channel (111) is used for blank control, the other longitudinal flow channels (111) are used for modifying target antibodies, and each longitudinal flow channel (111) is provided with an inlet A and an outlet A; The second flow channel group (120) comprises at least two parallel transverse flow channels (121), the transverse flow channels (121) are used for loading samples and fluorescent marks, and at least two transverse flow channels (121) share an inlet B and an outlet B; The transverse runner (121) is vertically attached to the decoration mark of the longitudinal runner (111), and an array point (115) is formed at the intersection of the transverse runner (121) and the decoration mark of the longitudinal runner (111); The first runner group (110) is used for being packaged on the substrate and leaving a modified mark after specifically capturing food allergen in food extract or aerosol, and the second runner group (120) is used for being packaged on the substrate after removing the first runner group (110) and loading and marking the captured food allergen by using a fluorescent marked detection antibody.
  2. 2. The array-type food allergen detection microfluidic chip according to claim 1, wherein the number of the longitudinal flow channels (111) is five, wherein four of the longitudinal flow channels (111) are used for modifying one of four target antibodies, respectively; wherein the four target antibodies comprise egg ovalbumin, ovomucoid, lysozyme and tropomyosin derived from crustacean scylla paramamosain.
  3. 3. The array type food allergen detection microfluidic chip according to claim 1, wherein a fishbone structure (121 a) is disposed on a side of the lateral flow channel (121) away from the substrate, so as to uniformly generate eddy currents in the internal fluid and promote uniform mixing of targets in the fluid.
  4. 4. An array-type food allergen detection micro-fluidic chip according to claim 3, characterized in that the number of the lateral flow channels (121) is four, and the four lateral flow channels (121) share one inlet B and one outlet B.
  5. 5. The array type food allergen detection micro-fluidic chip according to claim 1, wherein the substrate is made of glass, and the first flow channel group (110) and the second flow channel group (120) are respectively integrated on PDMS.
  6. 6. An adaptive microfluidic chip holder for holding the arrayed food allergen detection microfluidic chip according to any one of claims 1 to 5; The clamp comprises a loading groove (210), an adaptive clamping plate (220) and a fixing bolt (230); An opening is formed in the top of the loading groove (210), the opening is used for placing the array type food allergen detection microfluidic chip and the self-adaptive clamping plate (220), and windows (211) for accommodating the fixing bolts (230) are formed in the side walls of the loading groove (210) according to different heights; The adaptive clamping plate (220) comprises two layers of clamping plates (221), and a plurality of elastic pieces (222) arranged between the clamping plates (221).
  7. 7. The adaptive microfluidic chip holder according to claim 6, wherein a longitudinal flow channel loading window and a transverse flow channel loading window (212) are formed at the bottom of the loading slot (210); the longitudinal runner loading window is used for exposing the inlet A and the outlet A; The transverse runner loading window is used for exposing the inlet B and the outlet B.
  8. 8. A detection system comprising an array-type food allergen detection microfluidic chip according to any one of claims 1 to 5.
  9. 9. The detection system according to claim 8, further comprising an aerosol-generating assembly (300), and a fixture (200) according to claim 7, the array-type food allergen detection microfluidic chip (100) being disposed within the fixture (200); The aerosol generating assembly (300) comprises a ceramic ultrasonic atomizing sheet (310), an atomizing cavity cover (320), a transmission diaphragm (330) and an atomizing cavity (340) which are sequentially arranged; The ceramic ultrasonic atomization sheet (310) is used for generating high-frequency vibration, transmitting the high-frequency vibration to the transmission diaphragm (330) through medium liquid, and further transmitting the high-frequency vibration to the solution to be tested in the atomization cavity (340), so as to promote the vibration atomization of the solution to be tested, and generate aerosol; The nebulization chamber (340) comprises a lower solution storage area (341) and an upper aerosol area (342), the diameter of the solution storage area (341) being smaller than the diameter of the aerosol area (342); the solution storage area (341) is used for storing and atomizing solution; The top of the aerosol zone (342) is provided with a carrier gas inlet and an aerosol outlet, and the carrier gas is used for fully contacting with the aerosol in the atomization cavity (340) after entering through the inlet, and finally carrying the aerosol to enter the detection device from the outlet for reaction and analysis.
  10. 10. The detection system according to claim 9, wherein the transmission diaphragm (330) is further provided with O-rings (350) on both sides, respectively.

Description

Array type food allergen detection micro-fluidic chip, clamp and detection system Technical Field The invention relates to the technical field of allergen detection, in particular to an array type food allergen detection microfluidic chip, a clamp and a detection system. Background Food allergy is a pathological reaction of a patient after the patient contacts with food allergen, which may damage a plurality of organs such as respiratory system, digestive system, skin and the like of the patient, and may cause anaphylactic shock and even death when serious. The incidence of food allergy has been on an increasing trend year by year over the last decades. Therefore, development of a rapid detection method for food allergens remains an urgent task. Disclosure of Invention The invention aims to provide an array type food allergen detection microfluidic chip with simple operation and high sensitivity, and simultaneously provides a packaging technology for non-chemical covalent bonding of the microfluidic chip so as to solve the problem of irreversible chemical covalent bonding of the microfluidic chip. In addition, the invention also provides a method for converting the target to be detected in the solution into an aerosol state so as to simulate an allergen aerosol generation mechanism in the food processing or heating process and improve the allergen detection sensitivity. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: the invention provides an array type food allergen detection micro-fluidic chip, which comprises a first micro-fluidic chip and a second micro-fluidic chip which share a substrate, wherein the first micro-fluidic chip comprises a first runner group arranged on the substrate, the second micro-fluidic chip comprises a second runner group arranged on the substrate, the first runner group comprises at least two longitudinal runners which are arranged in parallel, one longitudinal runner is used for blank comparison, the other longitudinal runners are used for modifying target antibodies, each longitudinal runner is provided with an inlet A and an outlet A, the second runner group comprises at least two transverse runners which are arranged in parallel, the transverse runners are used for loading and fluorescent marking, the at least two transverse runners share an inlet B and an outlet B, the transverse runners are vertically attached to modification marks of the longitudinal runners, and an array point is formed at the intersection of the transverse runners and the modification marks of the longitudinal runners. The two microfluidic chips are sequentially packaged in the substrate (namely, the first microfluidic chip is packaged and then the second microfluidic chip is packaged), and a detection area is formed at the intersection of the flow channels to form array points, so that simultaneous high-flux detection of several proteins is realized. The microfluidic chip flow channel width may be on the order of microns. The array type microfluidic chip is packaged by the self-adaptive microfluidic chip clamp, the microfluidic chip can be guaranteed to have good sealing performance without covalent bonding, and the packaging process is reversible, so that different microfluidic chips can be assembled on the same substrate in sequence. Further, the number of the longitudinal flow channels is five, wherein four longitudinal flow channels are used for respectively modifying one of four target antibodies, and the four target antibodies comprise OVA, OVO, LYS and TM antibodies. Furthermore, a fishbone structure is arranged on one side of the transverse flow channel far away from the substrate, so that vortex flow is uniformly generated in the internal fluid, and further uniform mixing of targets in the fluid is promoted. Further, the number of the transverse flow channels is four, and the four transverse flow channels share one inlet B and one outlet B. Further, the substrate is made of glass, and the first runner group and the second runner group are respectively integrated on PDMS. More specifically, the substrate is a glass slide. The invention provides a clamp for clamping the array type food allergen detection microfluidic chip, which comprises a loading groove, an adaptive clamping plate and a fixing bolt, wherein an opening is formed in the top of the loading groove and used for placing the array type food allergen detection microfluidic chip and the adaptive clamping plate, windows used for accommodating the fixing bolt are formed in the side walls of the loading groove according to different heights, and the adaptive clamping plate comprises two layers of clamping plates and a plurality of elastic pieces arranged between the clamping plates. Different windows are at different distances from the bottom of the loading slot to better adapt to microfluidic chips of different thicknesses. The self-adaptive clamping plate can self-adaptively adjust the