CN-115992046-B - Microorganism automatic detection turntable and device

CN115992046BCN 115992046 BCN115992046 BCN 115992046BCN-115992046-B

Abstract

The invention provides a microorganism automatic detection turntable and a microorganism automatic detection device, which comprise a top plate, a middle layer plate, a one-way conduction structure and a bottom plate, wherein the middle layer plate, the one-way conduction structure and the bottom plate are sequentially and fixedly connected from top to bottom, the top plate is in sliding connection with the middle layer plate, a sample cavity is arranged on the top plate, a plurality of detection structures distributed along the circumference are further arranged on the top plate, the detection structures comprise a nucleic acid extraction cavity, a washing liquid cavity and a nucleic acid detection cavity, the sample cavity is communicated with the nucleic acid extraction cavity, a plurality of bacteria interception structures distributed along the circumference are arranged on the middle layer plate and used for intercepting bacteria in a solution to be detected, the waste liquid is discharged to the bottom plate through the one-way conduction structure, the one-way conduction structure is used for preventing the waste liquid discharged to the bottom plate from flowing back to the bacteria interception structures, and a plurality of waste liquid storage structures distributed along the circumference are arranged on the bottom plate. The invention realizes the rapid and simple detection of the food-borne pathogenic bacteria, and reduces the dependence on large instruments and equipment and technicians.

Inventors

LIN JIANHAN
WANG SIYUAN
CAI GAOZHE
QI WUZHEN
DUAN HONG

Assignees

中国农业大学

Dates

Publication Date: 20260508
Application Date: 20211018

Claims (10)

1. The automatic microorganism detection turntable is characterized by comprising a top plate, a middle layer plate, a unidirectional conduction structure and a bottom plate which are sequentially and fixedly connected from top to bottom, wherein the top plate is in sliding connection with the middle layer plate; The top plate is provided with a sample chamber, and the top plate is also provided with a plurality of detection structures distributed along the circumference; The detection structure comprises a nucleic acid extraction chamber, a washing liquid chamber and a nucleic acid detection chamber, and the sample chamber is communicated with the nucleic acid extraction chamber; The middle layer plate is provided with a plurality of bacteria interception structures distributed along the circumference, the bacteria interception structures are positioned below the nucleic acid extraction chamber, and the bacteria interception structures are used for intercepting bacteria in a solution to be detected and discharging waste liquid to the bottom plate through the unidirectional conduction structures; The one-way conduction structure is used for preventing waste liquid discharged to the bottom plate from flowing back to the bacteria retaining structure; The bottom plate is provided with a plurality of waste liquid storage structures distributed along the circumference, and the waste liquid storage structures are used for storing waste liquid.
2. The automated microorganism detection turntable of claim 1, wherein a layer of silicone oil is disposed between the top plate and the middle layer of sheet.
3. The automated microorganism detection turntable of claim 2, wherein the middle plate is provided with a groove matching the top plate, and the bacteria-retaining structure is located at the bottom of the groove.
4. A microbiological automated detection carousel according to any of claims 1-3, wherein the bacterial entrapment feature comprises an entrapment cavity having an FTA card disposed therein, the entrapment cavity being located below the nucleic acid extraction cavity, the bottom of the entrapment cavity being in communication with the unidirectional conducting feature.
5. A microbiological automation testing turntable according to any of claims 1-3, wherein the unidirectional conducting structure comprises a membrane, a plurality of unidirectional valve openings distributed along the circumference are arranged on the membrane, the unidirectional valve openings are positioned between the bacteria retaining structure and the waste liquid storage structure, and the unidirectional valve openings are in one-to-one correspondence with the bacteria retaining structure.
6. A microbiological automated detection carousel according to any of claims 1-3, wherein the waste storage structure comprises a flow-through chamber and a waste chamber, the flow-through chamber being in communication with the waste chamber, the flow-through chamber being located below the bacteria-retaining structure.
7. A microbiological automated detection rotor according to any one of claims 1 to 3, wherein the circumferences of the nucleic acid extraction chamber, the wash chamber and the nucleic acid detection chamber coincide with the center of the circumference of the bacterial entrapment feature and are the same size and parallel to each other.
8. The utility model provides a microorganism automation detection device, its characterized in that, including the detection box, with the case lid that detects the box and be connected, rotate driving piece, heating piece, compress tightly the subassembly and the microorganism automation detection carousel of any one of claims 1-7, rotate driving piece fixed mounting in the box, microorganism automation detection carousel is installed on the detection box, rotate driving piece with microorganism automation detection carousel is connected, heating piece fixed mounting is in on the detection box, heating piece is used for heating microorganism automation detection carousel, compress tightly the subassembly and install on the case lid, compress tightly the subassembly and be located microorganism automation detection carousel's top.
9. The automated microorganism detection apparatus of claim 8, wherein the compression assembly comprises a compression drive and a compression plate, the compression drive being fixedly mounted to the cover, the compression plate being coupled to the compression drive.
10. The automated microorganism detection apparatus of claim 9, wherein a camera is further disposed on the cover, the camera being positioned between the platen and the cover.

Description

Microorganism automatic detection turntable and device Technical Field The invention relates to the technical field of microorganism detection, in particular to a microorganism automatic detection turntable and a microorganism automatic detection device. Background Food-borne pathogenic bacteria pollution occurs at various stages of food production, processing, storage, transportation and consumption chains, so that the corresponding detection means should meet the detection requirements in different detection scenes by virtue of the characteristics of simplicity in operation, rapidness and portability. However, existing methods for detecting food-borne pathogenic bacteria mainly include culture methods, immunological methods (e.g., ELISA), and molecular biological methods (e.g., PCR). The culture method is a gold standard method for bacteria detection, which has high accuracy and sensitivity, but takes a long time, has shorter ELISA detection time, better specificity, easy realization of high flux, lower sensitivity and higher false positive rate, and has shorter PCR detection time and higher sensitivity, but complicated nucleic acid extraction operation, and requires expensive instruments and equipment and professional technicians. Therefore, how to shorten the detection time, simplify the operation and reduce the dependence on large-scale instruments and technicians while ensuring high sensitivity is an important issue to be solved in the industry. Disclosure of Invention The invention provides a microorganism automatic detection turntable and a microorganism automatic detection device, which are used for solving the defects of long detection time, low detection sensitivity, complex operation and the like of a food-borne pathogenic bacteria detection method in the prior art, and having higher dependence on large-scale instrument equipment and technicians, realizing rapid and simple detection on the food-borne pathogenic bacteria while guaranteeing the sensitivity, and reducing the dependence on the large-scale instrument equipment and the technicians. The invention provides a microorganism automatic detection turntable, which comprises a top plate, a middle layer plate, a unidirectional conduction structure and a bottom plate, wherein the middle layer plate, the unidirectional conduction structure and the bottom plate are sequentially and fixedly connected from top to bottom, and the top plate is in sliding connection with the middle layer plate; The top plate is provided with a sample chamber, and the top plate is also provided with a plurality of detection structures distributed along the circumference; The detection structure comprises a nucleic acid extraction chamber, a washing liquid chamber and a nucleic acid detection chamber, and the sample chamber is communicated with the nucleic acid extraction chamber; The middle layer plate is provided with a plurality of bacteria interception structures distributed along the circumference, the bacteria interception structures are positioned below the nucleic acid extraction chamber, and the bacteria interception structures are used for intercepting bacteria in a solution to be detected and discharging waste liquid to the bottom plate through the unidirectional conduction structures; The one-way conduction structure is used for preventing waste liquid discharged to the bottom plate from flowing back to the bacteria retaining structure; The bottom plate is provided with a plurality of waste liquid storage structures distributed along the circumference, and the waste liquid storage structures are used for storing waste liquid. According to the microorganism automatic detection turntable provided by the invention, a silicon oil layer is arranged between the top plate and the middle layer plate. According to the automatic microorganism detection turntable provided by the invention, the middle layer plate is provided with the groove matched with the top plate, and the bacteria interception structure is positioned at the bottom of the groove. According to the automatic microorganism detection turntable provided by the invention, the bacteria interception structure comprises an interception cavity, an FTA card is arranged in the interception cavity, the interception cavity is positioned below the nucleic acid extraction cavity, and the bottom of the interception cavity is communicated with the unidirectional conduction structure. According to the automatic microorganism detection turntable provided by the invention, the unidirectional conduction structure comprises a film, a plurality of unidirectional valve holes distributed along the circumference are arranged on the film, the unidirectional valve holes are positioned between the bacteria interception structure and the waste liquid storage structure, and the unidirectional valve holes are in one-to-one correspondence with the bacteria interception structure. According to the automatic microorganism detection turn