CN-115999657-B - Finger-pressure transistor micro-channel chip and operation method thereof

Abstract

The invention provides a finger-pressure type transistor micro-channel chip, which comprises a body, a finger-pressure structure, a printed circuit board and a transistor chip. The body comprises a sample drop port, a detection flow channel, a sample drop port and an absorption groove. The sample dropping port pipeline of the sample is communicated with the detection flow channel. The absorption groove comprises an absorption pad. The sample dropping groove of the finger pressure structure is communicated with the sample dropping opening and a chip external space. The printed circuit board is fixedly arranged on the body. The transistor die circuit is connected to the printed circuit board. Therefore, the finger-pressure type transistor micro-channel chip is communicated with the space outside the chip through the sample dropping groove of the finger-pressure structure, so that the design of a closed cavity of the conventional finger-pressure type micro-channel chip can be omitted, the volume is reduced, and the preparation cost is saved. The invention also provides an operation method of the finger-pressure type transistor micro-channel chip.

Inventors

• LI WENBIN
• LIAO SHUXIAN

Assignees

• 医流体股份有限公司

Dates

Publication Date

20260512

Application Date

20211021

Claims (20)

1. 1. A finger-pressure transistor micro-fluidic chip, comprising: A body, comprising: A sample dropping port; a detecting flow channel having a first end and a second end, wherein the first end is communicated with the sample dropping port; a sample dropping port connected to the detection flow channel via pipeline, and An absorption groove communicated with the second end of the detection flow channel, wherein the absorption groove comprises an absorption pad; The finger pressing structure is arranged on the body and provided with a sample dripping groove, wherein the sample dripping groove is communicated with the sample dripping opening and is communicated with an external space of a chip, and the finger pressing structure comprises: A first pressing piece comprising a first cavity, wherein the first pressing piece is made of a buffer material, and The second pressing piece is arranged between the body and the first pressing piece, wherein the second pressing piece comprises a second cavity which is correspondingly communicated with the first cavity to form the sample dripping groove, and the second pressing piece is made of a hard material; A printed circuit board fixedly arranged on the body and comprising a containing groove, and The transistor chip is embedded in the accommodating groove and is connected with the printed circuit board in a circuit mode, wherein the transistor chip comprises a plurality of working electrodes, and the transistor chip corresponds to the detection flow channel and enables the working electrodes to be exposed in the detection flow channel.
2. 2. The micro-fluidic channel chip of claim 1, wherein the printed circuit board is disposed on a surface of the body different from the finger structure.
3. 3. The finger transistor micro-fluidic chip as recited in claim 2, wherein the printed circuit board further comprises a reference electrode, the reference electrode being exposed in the drop-like opening.
4. 4. The finger transistor micro-channel chip of claim 1, wherein the transistor chip is an ion sensitive field effect transistor chip.
5. 5. The finger transistor micro-channel chip of claim 1, wherein the body further comprises: and the two welding grooves are respectively arranged at two sides of the detection flow channel, and are used for welding a plurality of electrodes of the printed circuit board and the working electrodes of the transistor chip together to form an electric conduction loop.
6. 6. The finger-pressure transistor micro-channel chip according to claim 1, wherein the first pressing piece is made of foam, silica gel, rubber or a combination thereof.
7. 7. The finger-pressure transistor micro-channel chip as defined in claim 1, wherein the sample drop is formed on the detection channel and located at a side of the detection channel near the second end.
8. 8. The finger-pressure transistor micro flow channel chip as set forth in claim 1, wherein the sample drop port and the sample drop port are disposed on the body separately from each other, and the sample drop port communicates with the detection flow channel through a connection flow channel.
9. 9. The finger-pressure transistor micro-channel chip as defined in claim 8, wherein an angle is formed between the long axis of the detection channel and the connection channel, and the angle is 10 ° to 90 °.
10. 10. The finger-pressure transistor micro-channel chip as defined in claim 1, wherein the body comprises a body surface, and the body is composed of an upper substrate, a pipeline substrate and a bottom plate in order from the body surface; The upper substrate, the pipeline substrate, the bottom plate and the transistor chip are sequentially laminated to form the detection flow channel; The upper substrate comprises a first substrate opening, the pipeline substrate comprises a first pipeline opening, the first substrate opening is correspondingly communicated with the first pipeline opening, and the first substrate opening, the first pipeline opening and the bottom plate are sequentially laminated to form the absorption groove.
11. 11. The finger transistor micro-channel chip of claim 10, wherein the opening size of the first substrate opening is smaller than the surface area of the absorption pad.
12. 12. The finger transistor micro-channel chip of claim 10, wherein the bottom plate, the printed circuit board and the transistor chip are bonded to each other, and the bottom plate comprises a reference electrode opening, a chip electrode opening and two solder slot openings.
13. 13. A method of operating a finger-pressure transistor microchannel chip comprising: Providing the finger-pressure transistor micro-channel chip as claimed in claim 1, wherein the finger-pressure transistor micro-channel chip is connected with a detector in a telecommunication manner; A first measuring step is carried out, wherein a first detection liquid is added into the sample dropping groove so that the first detection liquid is input from the sample dropping opening and fills the detection flow channel, and the detector is operated at the moment so that the printed circuit board is electrified and drives the transistor chip to measure a background value of each working electrode; Performing a first pressing and exhausting step, wherein the finger pressing structure is pressed and the sample dripping groove is closed, so that the first detection liquid is discharged from the detection flow channel and enters the absorption groove; A sample reaction step is carried out, wherein a sample to be detected is added into the sample dropping port of the sample, so that the sample to be detected fills the detection flow channel and reacts for a preset reaction time; Performing a second pressing and exhausting step, wherein the finger pressing structure is pressed and the sample dripping groove is closed, so that the to-be-detected sample is discharged from the detection flow channel and enters the absorption groove; A second measuring step of adding a second detection liquid into the sample drop slot to make the second detection liquid input from the sample drop port and fill the detection flow channel, and operating the detector to energize the printed circuit board and drive the transistor chip to measure a detection value of each working electrode And a data analysis step is carried out, wherein the background value and the detection value of each working electrode are analyzed to obtain a detection result of each working electrode.
14. 14. The method of operating a finger-pressure transistor micro flow channel chip as set forth in claim 13, wherein the first detection liquid is added in an amount a and the second detection liquid is added in an amount B, which satisfies the following condition: 2A≤B。
15. 15. the method of claim 13, wherein the printed circuit board is disposed on a surface of the body different from the finger structure.
16. 16. The method of claim 13, wherein the printed circuit board further comprises a reference electrode, the reference electrode being exposed in the drop-out port.
17. 17. The method of claim 13, wherein the transistor chip is an ion-sensitive field effect transistor chip.
18. 18. The method of claim 13, wherein the first pressing piece is made of foam, silica gel, rubber or a combination thereof.
19. 19. The method of claim 13, wherein the sample drop is formed on the detection channel and is located at a side of the detection channel near the second end.
20. 20. The method of claim 13, wherein the sample drop port and the sample drop port are disposed on the body separately from each other, and the sample drop port communicates with the detection flow channel through a connection flow channel.

Description

Finger-pressure transistor micro-channel chip and operation method thereof Technical Field The present invention relates to a micro flow channel chip (wafer) and an operating method thereof, and more particularly, to a finger-pressure type transistor micro flow channel chip for detecting by using a transistor and an operating method thereof. Background In view of the development of biomedicine, how to rapidly and accurately diagnose diseases in vitro has become the target of development in the related art, and devices for rapidly performing detection have also been vigorously developed. The biosensor (biosensing) is an integrated analysis system developed by applying the characteristics of biological reaction and specificity identification, and comprises a biological identification device and a signal transmission and conversion device. When the detection is carried out, the substance to be detected reacts with the biological recognition device and generates light, heat, quality or electrochemical signals, and at the moment, the signal transmission and conversion device can convert the light, heat, quality or electrochemical signals into output signals for subsequent analysis and detection. In addition, the types of the biosensors used for detection in the market are diversified, and the matching and the use of the different types of the biological recognition devices and the signal transmission and conversion devices can enable the biosensors to meet different detection requirements. Therefore, how to provide a biosensor with low cost and high detection efficiency, and further apply the biosensor to biomedical related tests is an objective of development of related academia and industry. Disclosure of Invention An embodiment of the present invention provides a finger-pressing transistor micro-channel chip, which includes a body, a finger-pressing structure, a printed circuit board, and a transistor chip. The body comprises a sample drop port, a detection flow channel, a sample drop port and an absorption groove. The detection flow channel is provided with a first end and a second end, wherein the first end is communicated with the sample dropping port. The sample dropping port pipeline of the sample is communicated with the detection flow channel. The absorption groove is communicated with the second end of the detection flow channel, wherein the absorption groove comprises an absorption pad. The finger pressing structure is arranged on the body and provided with a sample dripping groove, wherein the sample dripping groove is communicated with the sample dripping opening and is communicated with the external space of a chip, and the finger pressing structure comprises a first pressing piece and a second pressing piece. The first pressing piece comprises a first cavity, wherein the first pressing piece is made of a buffer material. The second pressing piece is arranged between the body and the first pressing piece, wherein the second pressing piece comprises a second hole cavity which is correspondingly communicated with the first hole cavity to form the sample dripping groove, and the second pressing piece is made of a hard material. The printed circuit board is fixedly arranged on the body and comprises a containing groove. The transistor chip is embedded in the accommodating groove and is connected with the printed circuit board through a circuit, wherein the transistor chip comprises a plurality of working electrodes, and the transistor chip corresponds to the detection flow channel and enables the working electrodes to be exposed in the detection flow channel. According to the finger-pressure transistor micro-channel chip of the foregoing embodiment, the printed circuit board may be disposed on a surface of the body different from the finger-pressure structure. The finger-pressure transistor micro-channel chip according to the above embodiment may further comprise a reference electrode, wherein the reference electrode is exposed in the drop sample port. The finger-pressure type transistor micro flow channel chip according to the foregoing embodiment, wherein the transistor chip may be an ion-sensitive field effect transistor (ion-SENSITIVE FIELD-effect transistor, ISFET) chip. According to the aforementioned embodiment, the body may further include two soldering grooves, the two soldering grooves are respectively disposed on two sides of the detection flow channel, and the two soldering grooves are used for soldering the plurality of electrodes of the printed circuit board and the working electrode of the transistor chip together to form an electrical conduction loop. According to the aforementioned embodiment, the first pressing piece may be made of foam, silica gel, rubber or a combination thereof. According to the finger-pressure transistor micro-channel chip of the foregoing embodiment, the sample drop port may be opened on the detection channel and located at a side of the detection channel near t