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CN-116063437-B - Rice RNA apparent modification related gene OsREF and synergistic improvement yield and stress resistance application thereof

CN116063437BCN 116063437 BCN116063437 BCN 116063437BCN-116063437-B

Abstract

The invention discloses OsREF protein, a coding gene and application thereof. The protein is any one of the following proteins, (a 1) the amino acid sequence of which is shown as a sequence 2, (a 2) the protein which is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown as the sequence 2 and has the same function, (a 3) the protein which has more than 80 percent of identity with the amino acid sequence defined in any one of (a 1) to (a 2) and has the same function, and (a 4) the fusion protein which is obtained by connecting labels at the tail ends of the protein defined in any one of (a 1) to (a 3). The protein can be improved by various characteristics of plants.

Inventors

  • GU XIAOFENG
  • HU GUIHUA
  • LI CONG

Assignees

  • 中国农业科学院生物技术研究所

Dates

Publication Date
20260512
Application Date
20221125

Claims (5)

  1. 1. The application of the protein is characterized in that the protein is applied to any one of the following applications, (1) Regulating and controlling the plant yield; (2) Regulating and controlling plant salt stress tolerance; (3) Regulating and controlling the chlorophyll content of the plants; The protein is any one of the following: (a1) The amino acid sequence is a protein shown in a sequence 2; (a2) A fusion protein obtained after the tag is attached to the end of the protein defined in (a 1); The plant is rice.
  2. 2. The application of the biological material is characterized in that the biological material is applied to any one of the following, (1) Regulating and controlling the plant yield; (2) Regulating and controlling plant salt stress tolerance; (3) Regulating and controlling the chlorophyll content of the plants; The biological material is any one of the following, C1 A nucleic acid molecule encoding the protein of claim 1; c2 An expression cassette comprising c 1) said nucleic acid molecule; c3 A recombinant vector comprising c 1) said nucleic acid molecule, or a recombinant vector comprising c 2) said expression cassette; c4 A recombinant microorganism comprising c 1) said nucleic acid molecule, or a recombinant microorganism comprising c 2) said expression cassette, or a recombinant microorganism comprising c 3) said recombinant vector; c5 A transgenic plant cell line comprising c 1) said nucleic acid molecule, or a transgenic plant cell line comprising c 2) said expression cassette; c6 A transgenic plant tissue comprising c 1) said nucleic acid molecule, or a transgenic plant tissue comprising c 2) said expression cassette; c7 A transgenic plant organ comprising c 1) said nucleic acid molecule, or a transgenic plant organ comprising c 2) said expression cassette; c8 A nucleic acid molecule which reduces or inhibits the expression of the nucleic acid molecule according to c 1); c9 A expression cassette, recombinant vector, recombinant microorganism or transgenic plant cell line comprising the nucleic acid molecule of c 8); The plant is rice.
  3. 3. The method according to claim 2, wherein c 1) the nucleic acid molecule is a DNA molecule of sequence 3.
  4. 4. A method for plant breeding, characterized in that the method is M1, M2 or M3, Said M1 comprises increasing and/or increasing the expression of a gene encoding a protein according to claim 1 and/or the content of a protein according to claim 1 in a starting plant, resulting in a plant having at least one of the following properties: 1) An increase in plant yield compared to the starting plant; 2) An increase in plant salt stress tolerance compared to the starting plant; 3) The chlorophyll content of the plant is increased compared to the starting plant; Said M2 comprises inhibiting or reducing the expression of a gene encoding a protein according to claim 1 and/or the content of a protein according to claim 1 in a starting plant, resulting in a plant having at least one of the following properties: 1) A reduced plant height compared to the starting plant; 2) Reduced plant yield compared to the starting plant; 3) Reduced salt stress tolerance in plants compared to the starting plant; 4) A reduced chlorophyll content in the plant compared to the starting plant; The M3 comprises deleting G at 210 th position or inserting A after 210 th position of the coding sequence described in the sequence 3 in genome DNA of a starting plant to obtain a plant with at least one of the following characteristics: 1) A reduced plant height compared to the starting plant; 2) Reduced plant yield compared to the starting plant; 3) Reduced salt stress tolerance in plants compared to the starting plant; 4) A reduced chlorophyll content in the plant compared to the starting plant; The plant is rice.
  5. 5. A method for preparing a transgenic plant is characterized in that the preparation method is M4 or M5, Said M4 comprising the step of introducing into a starting plant an agent that increases and/or enhances the expression of a gene encoding a protein as defined in claim 1 and/or the content of a protein as defined in claim 1, resulting in a transgenic plant, said transgenic plant having at least one of the following properties compared to said starting plant: 1) Increased yield of transgenic plants compared to said starting plants; 2) The transgenic plant has increased salt stress tolerance compared to the starting plant; 3) The transgenic plant has an increased chlorophyll content compared to the starting plant; said M5 comprising the step of introducing into a starting plant an agent that reduces and/or reduces the expression of a gene encoding a protein according to claim 1 and/or the content of a protein according to claim 1, resulting in a transgenic plant, said transgenic plant having at least one of the following properties compared to said starting plant: 1) A reduced plant height of the transgenic plant compared to the starting plant; 2) Reduced yield of transgenic plants compared to the starting plant; 3) Reduced salt stress tolerance of the transgenic plant compared to the starting plant; 4) A reduced chlorophyll content in the transgenic plant compared to the starting plant; The plant is rice.

Description

Rice RNA apparent modification related gene OsREF and synergistic improvement yield and stress resistance application thereof Technical Field The invention relates to the technical field of biology, in particular to OsREF protein, and a coding gene and application thereof. Background Rice (Oryzasativa) is one of the important food crops, fostering about one half of the world's population. The popularization of semi-dwarf breeding in the 60 s of the last century leads to remarkable improvement of the worldwide rice yield. In recent 30 years, although rice heterosis utilization and new variety cultivation have greatly progressed in rice yield improvement, the improvement of yield still cannot meet the requirements of growing population for grains. The population size of 2050 is expected to reach 89 billion, which means that the grain production in 2050 needs to be improved by 50%. In the face of the severe population growth, the environment is continuously worsened, the arable area is continuously reduced, and how to effectively improve the rice unit yield is a very important task in agricultural production. The adverse stress is the main reason for reducing the yield and the quality of grains in China, and severely restricts the sustainable development of agriculture in China and even national agriculture. In current knowledge, stress resistance and high yield are often not compatible. The aim of the efforts of laboratory researchers is to 'combine' with reality and demand, discover stress-resistant gene resources of crops, and cultivate new varieties with stress resistance, high yield and high quality. Epigenetic refers to the occurrence of heritable changes in gene expression without altering the DNA, RNA or protein sequence. Methylation can occur on DNA and RNA, and is involved in various aspects such as growth and development, gene expression regulation and control, response to environmental signals and the like, and is the basis of biological character diversity. DNA methylation and mRNA methylation are hot flashes for research as new epigenetic modifications. At present, an intelligent design breeding system centering on the epigenetic theory and technology provides a brand new way for cultivating good varieties of crops. Disclosure of Invention One of the purposes of the present invention is a protein. The invention provides a protein, which is any one of the following proteins, (A1) The amino acid sequence is a protein shown in a sequence 2; (a2) A protein which has the same function and is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown in the sequence 2; (a3) A protein having 80% or more identity to the amino acid sequence defined in any one of (a 1) to (a 2) and having the same function; (a4) A fusion protein obtained by ligating a tag to the terminal of the protein defined in any one of (a 1) to (a 3). The above protein was designated OsREF protein. Biological materials related to the above proteins are also within the scope of the present invention, which biological materials are any of, C1 Nucleic acid molecules encoding the above proteins; c2 An expression cassette comprising c 1) said nucleic acid molecule; c3 A recombinant vector comprising c 1) said nucleic acid molecule, or a recombinant vector comprising c 2) said expression cassette; c4 A recombinant microorganism comprising c 1) said nucleic acid molecule, or a recombinant microorganism comprising c 2) said expression cassette, or a recombinant microorganism comprising c 3) said recombinant vector; c5 A transgenic plant cell line comprising c 1) said nucleic acid molecule, or a transgenic plant cell line comprising c 2) said expression cassette; c6 A transgenic plant tissue comprising c 1) said nucleic acid molecule, or a transgenic plant tissue comprising c 2) said expression cassette; c7 A transgenic plant organ comprising c 1) said nucleic acid molecule, or a transgenic plant organ comprising c 2) said expression cassette; c8 A nucleic acid molecule which reduces or inhibits the expression of the nucleic acid molecule according to c 1); c9 An expression cassette, a recombinant vector, a recombinant microorganism or a transgenic plant cell line comprising the nucleic acid molecule of c 8). Alternatively, according to the above-mentioned biological material, The nucleic acid molecule of c1 is a DNA molecule as shown in any one of the following: d1 The nucleotide sequence is a DNA molecule shown as a sequence 1 in a sequence table; d2 A coding sequence is a DNA molecule shown as a sequence 3 in a sequence table; d3 A DNA molecule which has 90% or more identity to the nucleotide sequence defined in d 1) or d 2), is derived from water rice and encodes the protein of claim 1; d4 A DNA molecule which hybridizes under stringent conditions to the nucleotide sequence defined in d 1) or d 2) and which codes for a protein according to claim 1. Alternatively, the recombinant