CN-116144792-B - SNP molecular marker related to pig backfat thickness and application thereof

CN116144792BCN 116144792 BCN116144792 BCN 116144792BCN-116144792-B

Abstract

The invention discloses a SNP molecular marker related to pig backfat thickness, which is a T > C mutation at 66885632bp locus of chromosome 5 of Sscoffa 11.1 version of international pig genome or a T > C mutation represented by M at 106 locus shown as SEQ ID NO.1, and the molecular marker is obviously related to pig backfat thickness property. By optimizing the dominant allele of the SNP molecular marker, the dominant allele frequency can be increased generation by generation, the backfat thickness of the core group breeding pigs is reduced, and the improvement progress of the carcass traits related to the breeding pigs is accelerated, so that the economic benefit of breeding of the breeding pigs is effectively improved.

Inventors

YE YONG
LI SHAOYUN
Huang Ruqu
Jian Yunhua
LI JIE
YANG JIE
LU PENGYUN
GUAN XIANG

Assignees

广东广垦畜牧集团股份有限公司
广东广垦畜牧工程研究院有限公司

Dates

Publication Date: 20260505
Application Date: 20230117

Claims (3)

1. An application of a product for detecting SNP molecular markers in breeding low backfat thick pig breeds, wherein the SNP loci of the SNP molecular markers are positioned at 66885632bp of chromosome 5 of the international pig genome Sscofa 11.1 version, the base polymorphism is T or C, and the pig breeds are pure white pigs, long white pigs or Duroc pigs, and the application comprises the following steps: 1) Detecting the SNP molecular markers in the reserve breeding pigs; 2) Selecting and reserving individual breeding pigs with SNP molecular marker genotypes of TT or TC in the step 1), and eliminating CC genotype individuals; 3) The individuals screened in the step 2) are used as breeding pigs, the breeding pig individuals with the SNP molecular marker genotype of TT or TC in the step 1) are continuously selected and remained in offspring, CC genotype individuals are eliminated, and the frequency of the allele T in offspring pig groups is increased by generations, so that the low backfat thickness pig breeds are cultivated.
2. Application of a product for detecting SNP molecular markers in screening piglets with low backfat thickness genetic characteristics, wherein the SNP locus of the SNP molecular markers is positioned at 66885632bp of chromosome 5 of Sscoffa 11.1 version of the international pig genome, the nucleotide polymorphism is T or C, and the pig breeds are pure white pigs, long white pigs or Duroc pigs, and the application comprises the following steps: 1) Detecting the SNP molecular markers in the piglets to be screened; 2) And (2) when the genotype of the SNP molecular marker detected in the step (1) is TT or TC, the piglet to be screened has the low backfat thickness genetic character and is reserved, and when the genotype is CC, the piglet to be screened has the high backfat thickness genetic character and is eliminated.
3. A genetic improvement method for reducing backfat thickness in pigs, wherein said pig breeds are pure white pigs, long white pigs or duroc pigs, said method comprising the steps of: 1) Detecting SNP molecular markers in the reserve breeding pigs, wherein the SNP loci of the SNP molecular markers are positioned at 66885632bp of chromosome 5 of the international pig genome Sscoffa 11.1 version, and the base polymorphism is T or C; 2) Selecting and reserving individual breeding pigs with SNP molecular marker genotypes of TT or TC in the step 1), and eliminating CC genotype individuals; 3) The individuals screened in the step 2) are used as the breeding pigs, the breeding pig individuals with the SNP molecular marker genotype of TT or TC in the step 1) are continuously selected and remained in the offspring, CC genotype individuals are eliminated, and the frequency of the allele T in the offspring pig group is increased by the generation, so that the backfat thickness of the offspring pigs is reduced.

Description

SNP molecular marker related to pig backfat thickness and application thereof Technical Field The invention relates to the field of animal genetic breeding, in particular to an SNP molecular marker related to pig backfat thickness and application thereof. Background In the production process of live pigs, backfat thickness is a key index for evaluating the fattening effect of live pigs and the fat deposition capability of the pigs. The reduction of backfat thickness and the improvement of carcass lean meat percentage are important breeding targets for pig breeding work at home and abroad. In addition, the backfat thickness has a certain influence on the appearance and reproductive performance of the sow in the early stage, so that the backfat thickness of the sow is of great significance in predicting other characters. The backfat thickness of the pig belongs to the medium-high genetic trait, the genetic power is about 0.45 and is easy to measure, so that very remarkable effect can be achieved by improving the backfat thickness by a conventional breeding method. Currently, with the publication of pig whole Genome sequences and the development of high-density chips, the identification of molecular markers and candidate genes related to pig economic traits by whole Genome association analysis (Genome-wide association study, GWAS) is widely used. Therefore, the molecular marker related to the pig backfat thickness can be identified by adopting a GWAS analysis strategy, so that the genetic improvement progress of the pig backfat character is quickened, and the economic benefit of a pig farm is improved. Disclosure of Invention The invention aims to provide an SNP molecular marker related to pig backfat thickness and application thereof, so as to solve the problems. According to a first aspect of the present invention there is provided a SNP molecular marker associated with pig backfat thickness, the molecular marker being located at the T > C base mutation at the 66885632bp position on chromosome 5 of version 5 of the International pig genome Sscofa 11.1. The molecular marker is obviously related to the pig backfat thickness property, and a high-efficiency and accurate molecular marker assisted breeding technology can be established by using the molecular marker, so that the molecular marker is applied to the genetic improvement for reducing the backfat thickness of a breeding pig, thereby improving the meat (lean meat) production performance of pig groups, improving the economic profit of enterprises and increasing the core competitiveness. By optimizing the dominant allele of the SNP molecular marker, the dominant allele frequency can be increased generation by generation, the backfat thickness of the core group breeding pigs is reduced, and the improvement progress of the carcass traits related to the breeding pigs is accelerated, so that the economic benefit of breeding of the breeding pigs is effectively improved. In some embodiments, the gene sequence upstream and downstream of the SNP molecular marker site is shown in SEQ ID NO. 1, and the SNP molecular marker is located at the T > C base mutation represented by M at the 106 th site shown in SEQ ID NO. 1. According to a second aspect of the invention, there is provided the use of a SNP molecular marker in the cultivation of low backfat thick swine breeds. The molecular marker is a T > C mutation at 66885632bp locus of the chromosome 5 of the Sscoffa 11.1 version of the international pig genome or a T > C mutation represented by M at 106 locus as shown in SEQ ID NO. 1. The genotype of the reserve breeding pigs is screened, and the breeding pig individuals with TT or TC are selected for propagation, so that the frequency of the allele T in the offspring pig group is increased generation by generation, and the low backfat thickness pig variety is cultivated. Therefore, the backfat thickness of the breeding pigs can be reduced, so that the meat production performance of offspring pig groups is improved, the economic profit of enterprises is improved, and the core competitiveness is improved. In certain embodiments, the application comprises the steps of: 1) Detecting a T > C mutation at the 66885632bp locus of chromosome 5 or a T > C mutation represented by M at the 106 th locus as shown in SEQ ID NO. 1 in a replacement strain pig; 2) Selecting and reserving individual breeding pigs with TT or TC molecular marker genotypes in the step 1), and eliminating CC genotype individuals; 3) The individuals screened in the step 2) are used as breeding pigs, the breeding pig individuals with the molecular marker genotype TT or TC in the step 1) are continuously selected and remained in offspring, CC genotype individuals are eliminated, and the frequency of the allele T in offspring pig groups is increased by generations, so that low backfat thickness pig breeds are cultivated. According to a third aspect of the invention, there is provided the use of a SNP molecular ma