CN-116148491-B - Sample needle suction abnormality detection method and device and electronic equipment

CN116148491BCN 116148491 BCN116148491 BCN 116148491BCN-116148491-B

Abstract

The invention provides a method, a device and electronic equipment for detecting abnormal suction of a sample needle, which comprise the steps of obtaining a sensor self-checking pressure value, determining whether a sample adding system is in a normal state or an abnormal state according to the self-checking pressure value, performing liquid suction operation on the sample adding system based on the normal state, obtaining a sample needle suction pressure waveform, determining whether the sample needle is in the normal state, a suction state or a needle blocking state according to a liquid suction pressure waveform, obtaining a sample needle cleaning pressure waveform, and determining whether the sample needle is in the normal filling cleaning state, the abnormal non-filling cleaning state or the needle blocking cleaning state according to the cleaning pressure waveform. The sample needle suction abnormality detection method provided by the invention can realize real-time detection of various abnormal states and ensure the accuracy of test results.

Inventors

YANG SHUO
XI ZAIJUN
WANG YING
LU DEQIANG
YUAN XULONG
YANG SHOUSHENG
YANG JIAN

Assignees

武汉明德生物科技股份有限公司

Dates

Publication Date: 20260505
Application Date: 20221231

Claims (7)

1. A method for detecting abnormal aspiration of a sample needle, which is applied to a sample application system, comprising: acquiring a sensor self-checking pressure value, and comparing the self-checking pressure value with a preset self-checking pressure interval to determine whether the sample adding system is in a normal state or an abnormal state; performing liquid suction operation based on a sample adding system in a normal state, acquiring a sample needle suction pressure waveform, determining a suction mark pressure value according to the liquid suction pressure waveform, comparing the suction mark pressure value with a preset suction judgment pressure interval, and determining that the sample needle is in the normal state or the suction state; determining a needle blocking identification pressure value according to the imbibition pressure waveform, and comparing the needle blocking identification pressure value with a preset needle blocking judgment pressure interval to determine whether the sample needle is in a normal state or a needle blocking state; The method comprises the steps of performing cleaning operation based on a normal sample adding system, obtaining a sample needle cleaning pressure waveform, determining a cleaning filling identification pressure array according to the cleaning pressure waveform, comparing the filling identification pressure array with a preset filling judgment pressure interval, and determining whether the sample needle is in a normal filling cleaning state or an abnormal non-filling cleaning state; determining a cleaning blocking needle identification pressure array according to the cleaning pressure waveform, and comparing the cleaning blocking needle identification pressure array with a preset cleaning blocking needle judgment pressure interval to determine whether the sample needle is in a normal cleaning state or a blocking needle cleaning state; the pipetting operations include a sample pipetting operation, a reagent pipetting operation and a diluent pipetting operation; the liquid suction volume of the sample suction operation is smaller than a first preset volume value, the liquid suction volume of the reagent suction operation is larger than or equal to the first preset volume value and smaller than a second preset volume value, and the liquid suction volume of the diluent suction operation is larger than or equal to the second preset volume value; The method for acquiring the sample needle suction pressure waveform, determining a suction mark pressure value according to the suction pressure waveform, comparing the suction mark pressure value with a preset suction judgment pressure interval, and determining the normal working state or the suction state of the sample needle comprises the following steps: Acquiring a sample needle suction pressure waveform, wherein the suction pressure waveform comprises a sample suction pressure waveform, a reagent suction pressure waveform and a diluent suction pressure waveform; obtaining a sample suction starting pressure value and a sample suction judging pressure value according to the sample suction pressure waveform, subtracting the sample suction starting pressure value from the sample suction judging pressure value to obtain a sample suction mark pressure value, comparing the sample suction mark pressure value with a preset sample suction judging pressure interval, and determining a normal working state or a suction state of the sample needle when the sample is sucked; Obtaining a reagent suction starting pressure value and a reagent suction judging pressure value according to the reagent suction pressure waveform, subtracting the reagent suction starting pressure value from the reagent suction judging pressure value to obtain a reagent suction mark pressure value, comparing the reagent suction mark pressure value with a preset reagent suction judging pressure interval, and determining the normal working state or the suction state of the sample needle when the reagent is sucked; Obtaining a diluent suction initial pressure value and a diluent suction judgment pressure value according to the diluent suction pressure waveform, subtracting a diluent suction initial pressure value from the diluent suction judgment pressure value, comparing the diluent suction identification pressure value with a preset diluent suction judgment pressure interval, and determining the normal working state or the suction state of the sample needle when the diluent is sucked; The liquid suction pressure waveform comprises a sample suction pressure waveform, a reagent suction pressure waveform and a diluent suction pressure waveform, the suction identification pressure value comprises a sample suction identification pressure value, a reagent suction identification pressure value and a diluent suction identification pressure value, and the preset suction judgment pressure interval comprises a preset sample suction judgment pressure interval, a preset reagent suction judgment pressure interval and a preset diluent suction judgment pressure interval.
2. The method of detecting abnormal aspiration of a sample needle according to claim 1, wherein said determining a needle blocking identification pressure value from said aspiration pressure waveform comprises: and obtaining a imbibition starting pressure value and an imbibition flow velocity peak point pressure value according to the imbibition pressure waveform, and obtaining the blocking needle identification pressure value by subtracting the imbibition starting pressure value from the imbibition flow velocity peak point pressure value.
3. The method according to claim 2, wherein the preset needle blocking determination pressure intervals corresponding to the sample sucking operation, the reagent sucking operation and the diluent sucking operation are the same.
4. The method of claim 1, wherein determining a purge filling identification pressure array from the purge pressure waveform comprises: and acquiring a cleaning initial pressure value, a cleaning pressure maximum value and a cleaning pressure minimum value according to the cleaning pressure waveform, and respectively subtracting the cleaning initial pressure value from the cleaning pressure maximum value and the cleaning pressure minimum value to obtain the cleaning filling identification pressure array.
5. The method of claim 1, wherein determining a purge needle stick identification pressure array from the purge pressure waveform comprises: And acquiring a cleaning initial pressure value, a cleaning pressure maximum value and a cleaning end pressure value according to the cleaning pressure waveform, and respectively subtracting the cleaning initial pressure value from the cleaning pressure maximum value and the cleaning end pressure value to obtain the cleaning needle blocking identification pressure array.
6. A sample needle aspiration anomaly detection device, characterized in that it is applied to a sample application system, comprising: The self-checking module is used for acquiring a sensor self-checking pressure value, comparing the self-checking pressure value with a preset self-checking pressure interval, and determining whether the sample adding system is in a normal state or an abnormal state; The liquid suction and air suction detection module is used for performing liquid suction operation based on a sample adding system in a normal state, acquiring a sample needle suction pressure waveform, determining an air suction identification pressure value according to the liquid suction pressure waveform, comparing the air suction identification pressure value with a preset air suction judgment pressure interval, and determining that the sample needle is in the normal state or the air suction state; The liquid suction needle blocking detection module is used for determining a needle blocking identification pressure value according to the liquid suction pressure waveform, comparing the needle blocking identification pressure value with a preset needle blocking judgment pressure interval, and determining that the sample needle is in a normal state or a needle blocking state; The cleaning filling detection module is used for carrying out cleaning operation based on a normal sample adding system, acquiring a sample needle cleaning pressure waveform, determining a cleaning filling identification pressure array according to the cleaning pressure waveform, comparing the filling identification pressure array with a preset filling judgment pressure interval set, and determining that the sample needle is in a normal filling cleaning state or an abnormal non-filling cleaning state; the cleaning blocking needle detection module is used for determining a cleaning blocking needle identification pressure array according to the cleaning pressure waveform, comparing the cleaning blocking needle identification pressure array with a preset cleaning blocking needle judgment pressure interval, and determining that the sample needle is in a normal cleaning state or a blocking needle cleaning state; The liquid suction and suction detection module comprises a sample suction and suction detection sub-module, a reagent suction and suction detection sub-module and a diluent suction and suction detection sub-module; The sample suction detection submodule is used for acquiring a sample suction pressure waveform, acquiring a sample suction starting pressure value and a sample suction judging pressure value according to the sample suction pressure waveform, subtracting the sample suction starting pressure value from the sample suction judging pressure value to obtain a sample suction identification pressure value, comparing the sample suction identification pressure value with a preset sample suction judging pressure interval, and determining a normal working state or a suction state of the sample needle when the sample is sucked; the reagent suction detection submodule is used for acquiring a reagent suction pressure waveform, acquiring a reagent suction starting pressure value and a reagent suction judging pressure value according to the reagent suction pressure waveform, subtracting the reagent suction starting pressure value from the reagent suction judging pressure value to obtain a reagent suction identification pressure value, comparing the reagent suction identification pressure value with a preset reagent suction judging pressure interval, and determining the normal working state or the suction state of the sample needle when the reagent is sucked; The diluent suction detection submodule is used for obtaining a diluent suction pressure waveform, obtaining a diluent suction starting pressure value and a diluent suction judging pressure value according to the diluent suction pressure waveform, subtracting the diluent suction starting pressure value from the diluent suction judging pressure value to obtain a diluent suction identification pressure value, comparing the diluent suction identification pressure value with a preset diluent suction judging pressure interval, and determining the normal working state or the suction state of the sample needle during diluent suction.
7. An electronic device comprising a memory, a processor and a computer program stored on the memory and executable on the processor, characterized in that the processor implements the sample needle aspiration abnormality detection method according to any one of claims 1 to 5 when executing the program.

Description

Sample needle suction abnormality detection method and device and electronic equipment Technical Field The invention relates to the field of in-vitro diagnostic equipment detection, in particular to a method and a device for detecting abnormal suction of a sample needle and electronic equipment. Background In the in-vitro diagnosis industry, various impurities may exist in a sample encountered by the diagnosis equipment, so that the sample needle is blocked when the sample is sucked, and abnormal conditions such as air suction and the like directly cause inaccurate sucked sample quantity, so that the test result is unreliable. The method is particularly important for monitoring the states of a sample adding loop and a sample needle in real time, the existing detection is mainly used for judging the fixed value of the pressure in a pipeline, the function is single, the stability is weak, and the abnormal states which are easy to occur cannot be completely distinguished, namely, the sample surface is provided with bubble films, bubbles and empty suction caused by the fact that the sample adding needle does not enter a real liquid level to suck samples due to false detection of the liquid level, the sample needle is immersed too deeply, impurities exist in the sample, the sample is sucked to a separating gel or a clot when the sample is insufficient, or suction blockage is caused by the bottom of a pressing cup, the inner wall pipeline is filled with air due to insufficient cleaning liquid when the sample adding system is used for cleaning, and the inner wall pipeline is blocked when the sample adding system is used for cleaning. In summary, in the prior art, detection of the abnormal sucking state of the sample needle is not comprehensive enough, so that accuracy of a test result is affected. Disclosure of Invention In view of the foregoing, it is necessary to provide a method, a device and an electronic device for detecting abnormal suction of a sample needle, which solve the technical problem in the prior art that the accuracy of the test result is not high due to insufficient detection of abnormal suction state of the sample needle. In order to solve the above technical problems, in one aspect, the present invention provides a method for detecting abnormal suction of a sample needle, which is applied to a sample adding system, including: acquiring a sensor self-checking pressure value, and comparing the self-checking pressure value with a preset self-checking pressure interval to determine whether the sample adding system is in a normal state or an abnormal state; performing liquid suction operation based on a sample adding system in a normal state, acquiring a sample needle suction pressure waveform, determining a suction mark pressure value according to the liquid suction pressure waveform, comparing the suction mark pressure value with a preset suction judgment pressure interval, and determining that the sample needle is in the normal state or the suction state; determining a needle blocking identification pressure value according to the imbibition pressure waveform, and comparing the needle blocking identification pressure value with a preset needle blocking judgment pressure interval to determine whether the sample needle is in a normal state or a needle blocking state; The method comprises the steps of performing cleaning operation based on a normal sample adding system, obtaining a sample needle cleaning pressure waveform, determining a cleaning filling identification pressure array according to the cleaning pressure waveform, comparing the filling identification pressure array with a preset filling judgment pressure interval, and determining whether the sample needle is in a normal filling cleaning state or an abnormal non-filling cleaning state; and determining a cleaning blocking needle identification pressure array according to the cleaning pressure waveform, and determining that the sample needle is in a normal cleaning state or a blocking needle cleaning state according to comparison between the cleaning blocking needle identification pressure array and a preset cleaning blocking needle judging pressure interval. In some possible implementations, the pipetting operations include a sample pipetting operation, a reagent pipetting operation, and a diluent pipetting operation; The liquid suction volume of the sample suction operation is smaller than a first preset volume value, the liquid suction volume of the reagent suction operation is larger than or equal to the first preset volume value and smaller than a second preset volume value, and the liquid suction volume of the diluent suction operation is larger than or equal to the second preset volume value. In some possible implementations, the obtaining the sample needle suction pressure waveform, determining a suction mark pressure value according to the suction pressure waveform, comparing the suction mark pressure value with a preset suction j