CN-116179559-B - Cold-resistant gene of bumblebee and application thereof

Abstract

The invention belongs to the technical field of biology, and particularly relates to a bumblebee cold-resistant gene and application thereof. The bumblebee cold-resistant gene has a nucleotide sequence shown in SEQ No. 1. The bumblebee cold-resistant gene has a nucleotide sequence shown in SEQ No. 2. A recombinant DNA containing the above-mentioned bumblebee cold-resistant gene. An expression vector into which the above recombinant DNA is inserted, and which uses a microorganism, an animal cell or a plant cell as a host cell. The application of the above-mentioned bumblebee cold-resistant gene in the cultivation of cold-resistant bumblebee. The cold-resistant gene of the hornet and the application thereof have the advantages that 1, the cold-resistant gene is beneficial to cultivating the cold-resistant hornet varieties, the hornet can still provide pollination service when the temperature is low, the fruit and vegetable yield is improved, and 2, the screening and identification of the cold-resistant hornet varieties are facilitated.

Inventors

• GUO SHENGNAN
• MENG XIANFEI
• ZHANG PENG
• LUO SHUDONG
• SUN CHENG

Assignees

• 衡水沃蜂生物科技有限公司

Dates

Publication Date

20260512

Application Date

20220907

Claims (1)

1. 1. The application of the hornet cold-resistant gene in the cultivation of cold-resistant hornet is provided, wherein the hornet cold-resistant gene has a nucleotide sequence shown as SEQ No.1 or SEQ No. 2.

Description

Cold-resistant gene of bumblebee and application thereof Technical Field The invention belongs to the technical field of biology, and particularly relates to a bumblebee cold-resistant gene and application thereof. Background Bumblebee is a collective term for insects of the genus bumblebee (Bombus) of the family honeybee, and is an important pollinating insect. Currently about 250 species of bumblebees exist worldwide. The bumblebee (Bombusterrestris) is one species of bumblebee, and the bumblebee originated in Europe has been successfully domesticated by people, and can provide excellent pollination service for greenhouse crops, so that the bumblebee is widely applied worldwide, including in China. One problem currently encountered in agricultural production is that, when the air temperature is low in early spring, the land bumblebee currently used for pollination of crops does not fly out of the honeycomb to work basically, thus affecting the pollination rate of the crops in this period and directly affecting the yield of the crops. Therefore, cultivation of cold-resistant bumblebee varieties becomes an urgent problem to be solved. Disclosure of Invention The invention aims to improve the problems existing in the prior art, namely the invention discloses a bumblebee cold-resistant gene and application thereof. The technical proposal is that the bumblebee cold-resistant gene has a nucleotide sequence shown as SEQNo. 1. The bumblebee cold-resistant gene has a nucleotide sequence shown as SEQ No. 2. A recombinant DNA containing the above-mentioned bumblebee cold-resistant gene. An expression vector into which the above recombinant DNA is inserted, and which uses a microorganism, an animal cell or a plant cell as a host cell. The application of the above-mentioned bumblebee cold-resistant gene in the cultivation of cold-resistant bumblebee. The invention has the beneficial effects that the cold-resistant gene of the bumblebee and the application thereof have the following beneficial effects: 1. the cultivation of cold-resistant bumblebee varieties is facilitated, so that the bumblebee can still provide pollination service when the temperature is low, and the fruit and vegetable yield is improved; 2. is favorable for screening and identifying cold-resistant bumblebee varieties. Drawings Fig. 1a to 1c are schematic diagrams of comparing the temperature of the place bumblebee in asian habitat with that of the european habitat, from which it can be seen that the place bumblebee has a significantly lower temperature in asian habitat than in european habitat. FIG. 2 is a schematic representation of the natural selection of the Dioscorea opposita LOC100649067 gene in Asian populations, from which it can be seen that the Dioscorea opposita LOC100649067 gene has a high Fst value and a low pi value. FIG. 3 is a schematic representation of the natural selection of the Dioscorea opposita LOC100644218 gene in Asian populations, from which it can be seen that the Dioscorea opposita LOC100644218 gene has a high Fst value and a low pi value. The specific embodiment is as follows: the following detailed description of specific embodiments of the invention. Wild bumblebee is widely distributed worldwide, from western to continental europe and from eastern to Xinjiang in China. We collected a total of 55 wild bumblebee samples in Europe and Asia. Climate data for these bumblebee samples were downloaded in Worldclim database (https:// www.worldclim.org /) using ArcMap (v 10.2) software and statistically analyzed using Wilcoxon rank sum test. As shown in fig. 1a to 1c, the temperature of the living land bumblebee in asia is significantly lower than that of the living land bumblebee in continental europe, which indicates that the genome of the living land bumblebee in asia should harbor the cold-resistant gene. To mine cold tolerance genes contained in the population of bumblebees in asia: First, we performed genomic resequencing on these 55 samples of bumblebee from asia and european regions only (sequences are stored in GenBank: SRP 311478). The sequencing results were then aligned with the reference genome sequence of the bumblebee (GenBank: GCA_ 000214255.1) using BWA software, and the results of the alignment were analyzed using VCFtools software to calculate the Fst and pi values for the two populations. Finally, genes selected in asian populations of the bumblebee are screened. The intersection of the first 5% of Fst values with the last 5% of pi ratio (european population/asian population), i.e. genes selected in asian population, which are related to the environment in which the bumblebee adapts to asian habitat. We used KOBAS software to functionally annotate the identified selected genes and screen genes associated with cold tolerance. The Glycerophospholipid (GPL) component is very important for insect cold tolerance, we identified a total of 2 genes, LOC100649067 (gene shown in seqno. 1) and LOC100644218 (gene shown