CN-116183800-B - Detection and analysis method for aroma components in pomegranate

Abstract

The invention discloses a detection and analysis method of aroma components in pomegranate, which belongs to the technical field of chemical analysis and comprises the following steps of S1, placing a pomegranate sample in a headspace bottle, incubating the pomegranate sample, S2, capturing volatile substances of the pomegranate sample by adopting a MonoTrapTD suction-type aconite static headspace, S3, carrying out desorption sample injection on the collected substances by adopting a direct thermal desorption method after capturing, and introducing the desorbed substances into a gas chromatography-mass spectrometer for qualitative and quantitative analysis. The invention utilizes MonoTrapTD method to enrich the aroma components of the pomegranate, combines direct thermal desorption-gas chromatography-mass spectrometry (LN-GC-MS) technology, accurately analyzes and detects the types and the contents of the aroma components in the pomegranate, and establishes a certain theoretical reference for preparing and producing the related aroma type essence.

Inventors

• LIU JIAWEI

Assignees

• 雅迪香料(广州)有限公司

Dates

Publication Date

20260512

Application Date

20230324

Claims (2)

1. 1. The detection and analysis method for the aroma components in the pomegranates is characterized by comprising the following steps of: s1, placing a pomegranate sample in a headspace bottle, incubating the pomegranate sample at 45 ℃ for 120min, S2, adopting MonoTrapTD suction-type radix aconiti lateralis static headspace to collect volatile matters of a pomegranate sample, wherein MonoTrapTD suction-type radix aconiti lateralis is two-phase suction-type radix aconiti lateralis, and the two-phase suction-type radix aconiti lateralis is PDMS/Carb type suction-type radix aconiti lateralis; S3, after trapping is finished, carrying out desorption sample introduction on the collected substances by adopting a direct thermal desorption method, and introducing the desorption substances into a gas chromatography-mass spectrometer for qualitative and quantitative analysis, wherein the analysis conditions of the direct thermal desorption method are that a sample introduction port is subjected to temperature programming gasification sample introduction, sample introduction is not split, the initial temperature is 80 ℃, the temperature is increased to 250 ℃ at 300 ℃ per minute, the desorption temperature is 250 ℃ for 5 minutes, the desorption time is 5 minutes, in the gas chromatography-mass spectrometer, the GC conditions are that a HP-5MS capillary column is 60m multiplied by 0.25 mm multiplied by 0.25 mu m, the carrier gas is high-purity He, the flow rate is 1.5mL/min, the temperature programming is carried out, the initial temperature is 50 ℃ and is increased to 100 ℃ at 5 ℃ per minute, the temperature is increased to 140 ℃ at 4 ℃ and is maintained for 10 minutes, the temperature is increased to 180 ℃ at 4 ℃ and is maintained for 10 minutes at 5 ℃ and the temperature is increased to 250 ℃ at the temperature of 250 ℃ and the desorption time is maintained for 10 minutes, the running temperature is 300 ℃ and the mass ion source temperature is maintained at 230 ℃ to 150 ℃ and the mass rod is scanned at the temperature of 150 ℃ to the temperature of the quadrupole.
2. 2. The method according to claim 1, wherein in step S1, the food portion is selected from the group consisting of a sample of pomegranate and a food grinder.

Description

Detection and analysis method for aroma components in pomegranate Technical Field The invention belongs to the technical field of chemical analysis, and particularly relates to a detection and analysis method for aroma components in pomegranate. Background The pomegranate is a berry, is rich in nutrition, and has vitamin C1-2 times higher than that of apples and pears. The original product of the Western China, the Han dynasty goes into the original place. After the pomegranate is ripe, the whole body can be used, the pericarp can be used as a medicine, and the fruit can be eaten or pressed into juice. Has high nutritive value for the health of the elderly, so the elderly should eat the punica granatum frequently. Pomegranate is a precious berry with a high nutritional value. In the analysis of fruit aroma, in the aspect of enrichment, methods mainly comprise a sweeping and trapping method, a solid-phase microextraction method (SPME), a reduced pressure distillation extraction method, a simultaneous distillation extraction method, a headspace steam distillation extraction method, a stirring rod adsorption extraction method and the like. These methods all have the significant disadvantages of low trapping concentration, time and effort consumption, easy distortion, and significant influence from the saturated vapor pressure of water. With the development of materials science, new forms of adsorbent materials have been developed and applied to volatile component analysis studies, including solid phase extraction monolithic traps (MonoTrap). The solid phase extraction whole trapping agent adsorption-desorption method (MonoTrapTD) developed by Shimadzu GL SCIENCES company is a novel and most advanced adsorption medium developed based on the high specific surface area technology of silica gel whole column, wherein the three-dimensional silica monomer pores and the mesoporous network surface are subjected to C18 or PDMS modification end-capping, and simultaneously, graphite carbon black treated by a high-temperature process is further embedded in the surface of the material, and the pores and the mesoporous (carbon black pores) surface can provide a large specific surface area exceeding 150m 2/g, so that MonoTrapTD of the small mixed adsorbent has higher adsorption and desorption capacities. It is designed for simple and rapid concentration of fragrances, aromas and aromatic substances and can be used very simply for analysis of volatile and semi-volatile compounds for quality control, environmental and forensic applications. After MonoTrapTD is used for adsorbing volatile substances, the volatile substances can be desorbed through solvent elution and can be directly subjected to thermal desorption at a sample inlet, so that the application universality is strong. At present, monoTrapTD has not been applied to the research on the aroma components of the pomegranates by researchers. Disclosure of Invention In view of the above, the invention provides a method for detecting and analyzing aroma components in pomegranate, which adopts MonoTrapTD technology to detect and analyze aroma components in the pomegranate, researches the composition and content level of the aroma components in the pomegranate, and provides valuable data reference for researching and preparing the pomegranate flavor essence. The invention is realized by the following technical scheme: a detection and analysis method for aroma components in pomegranate comprises the following steps: s1, placing a pomegranate sample in a headspace bottle, and incubating the pomegranate sample; S2, adopting MonoTrapTD suction-type aconite to collect volatile matters of the pomegranate sample in a static headspace; and S3, after trapping is finished, carrying out desorption and sample injection on the collected substances by adopting a direct thermal desorption method, and introducing the desorbed substances into a gas chromatograph-mass spectrometer for qualitative and quantitative analysis. Compared with the prior art, the method for enriching the aroma components of the pomegranate by utilizing MonoTrapTD has the advantages that the method is used for enriching the aroma components of the pomegranate, and meanwhile, the direct thermal desorption-gas chromatography-mass spectrometry (LN-GC-MS) technology is combined, so that the variety and the content of the aroma components in the pomegranate can be accurately analyzed and detected without high-temperature heating of a pomegranate sample, and a certain theoretical reference is established for preparing and producing the relevant aroma type essence. Further, in step S1, the food portion is selected from the pomegranate sample and crushed by a food crusher. Further, in step S1, the incubation temperature is 45 ℃. Further, in step S1, the incubation time is 120min. Further, in step S2, monoTrapTD pieces are two-phase pieces, and two-phase pieces are PDMS/Carb pieces. Further, in the step S3, the analysis conditio