CN-116236558-B - Application of natural oyster zinc peptide product in preparation of medicine for preventing and treating enteritis

Abstract

The invention discloses application of a natural oyster zinc peptide product in preparing a medicine for preventing and treating enteritis. According to the invention, animal experiments prove that the natural oyster zinc peptide product has the functions of preventing and treating enteritis, and can improve the apparent symptoms of ulcerative colitis of mice caused by DSS by regulating the level of oxidative stress, relieving inflammatory reaction, maintaining intestinal barrier and reducing apoptosis, and can also regulate the intestinal flora and metabolic products of enteritis mice, so that intestinal inflammation is relieved. The natural oyster zinc peptide product used by the invention has good safety, lower cost and no side effect compared with the common anti-inflammatory and immunity-enhancing medicaments, and has remarkable effect of relieving enteritis. Therefore, the natural oyster zinc peptide product has good prospect in the aspect of developing medicines for preventing and treating enteritis.

Inventors

• LIN HUAN
• MIAO JINLAI
• QU CHANGFENG
• KE KE
• HE YINGYING
• LIANG CHEN

Assignees

• 中国海洋大学
• 自然资源部第一海洋研究所
• 广西科学院

Dates

Publication Date

20260512

Application Date

20220907

Claims (5)

1. 1. The application of a natural oyster zinc peptide product in preparing a medicament for preventing and treating enteritis, which is characterized in that the enteritis is ulcerative colitis, and the preparation method of the natural oyster zinc peptide product is as follows: (1) Removing shells of oysters, cleaning oyster meat, and mashing with deionized water with the volume of 2-4 times to obtain mashed tissue fluid; (2) Performing ultrasonic crushing on the tissue triturating liquid obtained in the step (1), and centrifuging the obtained tissue triturating liquid to collect supernatant, wherein the ultrasonic crushing power is 80W and the time is 20 min; (3) Spin-steaming the supernatant in the step (2), and freeze-drying to obtain a crude product of the natural oyster zinc peptide; (4) And (3) separating and purifying the crude product of the natural oyster zinc peptide in the step (3) to obtain the natural oyster zinc peptide product, wherein a Sephadex G-15 gel chromatographic column is adopted in the separation and purification, the length of the Sephadex G-15 gel chromatographic column is 100 cm, the diameter of the Sephadex G-15 gel chromatographic column is 2.6 cm, the eluent condition is pure water, and the flow rate is 0.5 mL/min.
2. 2. The use according to claim 1, wherein the medicament is capable of oral administration, intramuscular administration, intravenous administration, intraperitoneal administration, subcutaneous administration, intradermal administration.
3. 3. The use of claim 1, wherein the mode of administration of the medicament comprises topical administration.
4. 4. The use according to claim 1, wherein the natural oyster zinc peptide product contains 4 peptide fragments, and the amino acid sequences of the peptide fragments are EKSGPGPHCPRC, TVGPITGGGSHK, VIDTNKDRT and SGPSIVH respectively.
5. 5. A medicament comprising 1.25 g/kg to 5 g/kg of the natural oyster zinc peptide product of claim 1.

Description

Application of natural oyster zinc peptide product in preparation of medicine for preventing and treating enteritis Technical Field The invention belongs to the field of food health care, and in particular relates to application of a natural oyster zinc peptide product in preparing a medicine for preventing and treating enteritis. Background In recent years, inflammatory Bowel Disease (IBD) has become one of the most serious chronic diseases that endanger human health. Symptoms of IBD are often manifested by inflammation of the colon or rectum, ulcers, diarrhea, hematochezia, and weight loss, and a long course of disease. Among them, recurrence of Ulcerative Colitis (UC) is often accompanied by complications, severely affecting the quality of life of the patient. Research data show that the incidence of UC tends to rise year by year, especially in developed countries, but drugs for treating UC are often expensive and have many side effects, such as acne, abdominal pain, weight gain, diabetes, etc., so it is imperative to find functional components for treating or relieving UC from food-borne active substances. In order to explore the pathogenesis of IBD that may exist, new targets for disease treatment were sought, and different animal models were applied in experiments. Among these, DSS-induced UC animal models are most commonly used. Animals can develop UC-like symptoms by adding DSS to drinking water, which is one of the ideal animal models for studying the efficacy of IBD treatment. DSS has toxic effect on colon epithelial cells, can destroy intestinal barrier, increase intestinal permeability, promote inflammatory cytokines and toxins to enter intestinal tract through intestinal wall, activate related inflammatory signal pathway, and cause inflammatory injury, so that experimental animals show obvious symptoms such as weight reduction, diarrhea, hematochezia and hypoactivity. The method has the advantages of convenient operation, strong repeatability and most wide application in enteritis models. More and more research reports indicate that the beneficial effects of a large number of food ingredients, nutritional ingredients such as proteins, active peptides and the like on UC far exceed the traditional nutritional value. Proteins and the like can be used as carbon sources and nitrogen sources of intestinal microorganisms for growth, and the microorganisms can generate SCFAs after metabolism, thereby participating in regulation of inflammatory reactions. The functional research of oyster bioactive peptide is increasingly abundant, the biological utilization rate of zinc ions can be improved, and zinc element has certain anti-inflammatory activity. The natural oyster zinc peptide product prepared by the invention is prepared from crassostrea gigas, has rich raw materials, good safety and no side effect, and therefore, has great potential in the development of medicines for preventing and treating enteritis. Disclosure of Invention The invention provides an application of a natural oyster zinc peptide product in preparing a medicine for preventing and treating enteritis. The natural oyster zinc peptide product has rich and easily obtained raw materials, good safety and obvious enteritis improvement effect, and can regulate intestinal flora and metabolites of enteritis mice. In order to achieve the above purpose, the invention adopts the following technical scheme: The invention provides an application of a natural oyster zinc peptide product in preparing a medicine for preventing and treating enteritis. Furthermore, the natural oyster zinc peptide product contains 4 peptide fragments with better binding ability with zinc ions, and the amino acid sequences of the peptide fragments are EKSGPGPHCPRC, TVGPITGGGSHK, VIDTNKDRT and SGPSIVH respectively. Further, the preparation method of the natural oyster zinc peptide product comprises the following steps: (1) Removing shells of oysters, cleaning oyster meat, and mashing with deionized water with the volume of 2-4 times to obtain mashed tissue fluid; (2) Performing ultrasonic crushing on the tissue triturating liquid obtained in the step (1), and centrifugally collecting supernatant; (3) Spin-steaming the supernatant in the step (2), and freeze-drying to obtain a crude product of the natural oyster zinc peptide; (4) And (3) separating and purifying the crude product of the natural oyster zinc peptide in the step (3) to obtain the natural oyster zinc peptide product. Further, the ultrasonic crushing power in the step (2) is 70W-100W, and the time is 15 min-30 min. Further, the effective dose of the natural oyster zinc peptide product is 1.25 g/kg/bw-5 g/kg/bw. Furthermore, the natural oyster zinc peptide product can obviously reduce the weight loss of mice caused by DSS, improve the abnormal condition of fecal state and inhibit the increase of DAI scores of the mice. Furthermore, the natural oyster zinc peptide product can relieve colon appearance and internal