CN-116256473-B - Reed cadmium transport capacity assessment method based on plant functional characters

Abstract

The invention belongs to the technical field of soil remediation, and relates to a method for evaluating the cadmium transport capacity of reed based on plant functional properties. A method for evaluating the cadmium transport capacity of reed based on plant functional characters comprises the steps of measuring the base diameter, stomatal conductivity and transpiration rate of reed growing normally, and calculating a cadmium transport evaluation index R= (R 1 +R 2 +R 3 )/3 according to the base diameter, the stomatal conductivity and the transpiration rate, wherein R 1 、R 2 、R 3 is the transpiration rate evaluation index, the stomatal conductivity evaluation index and the base diameter evaluation index respectively, and evaluating the cadmium transport capacity of reed by utilizing the cadmium transport evaluation index. The method can evaluate the cadmium transporting capability of the reed by only measuring the partial functional properties of the reed without cadmium treatment, reduces cadmium pollution, is safe and environment-friendly, is beneficial to screening reed strains with high cadmium transporting capability, and can improve the application efficiency of the reed in heavy metal pollution restoration.

Inventors

• GUO WEIHUA
• LIU LELE
• JIANG DONGZI
• CUI ZHAOJIE

Assignees

• 山东大学

Dates

Publication Date

20260505

Application Date

20230427

Claims (4)

1. 1. A method for evaluating the cadmium transport capacity of reed based on plant functional properties is characterized by measuring the base diameter, air hole conductivity and transpiration rate of reed which grows normally, calculating a cadmium transport evaluation index R= (R 1 +R 2 +R 3 )/3 according to the base diameter, the air hole conductivity and the transpiration rate, wherein R 1 、R 2 、R 3 is the transpiration rate evaluation index, the air hole conductivity evaluation index and the base diameter evaluation index respectively, evaluating the cadmium transport capacity of reed by utilizing the cadmium transport evaluation index, calculating the average value of the transpiration rate of each genetic reed and the maximum value of the transpiration rate of all reed according to the measured transpiration rate, dividing the average value of the transpiration rate of each genetic reed by the maximum value of the transpiration rate of all reed to obtain the average value of the air hole conductivity of each genetic reed and the maximum value of the air hole conductivity in all reed according to the measured transpiration rate, dividing the average value of the air hole conductivity of each genetic reed by the maximum value of the genetic reed to obtain the average value of the gene type of the gene reed and the average value of the transpiration rate of all reed to obtain the base diameter of the genetic reed according to the average value of the genetic reed, and the gene type of the average value of the transpiration rate of all reed is calculated by the gene type of the gene reed.
2. 2. The method for evaluating cadmium transport capacity of reed based on plant functional properties according to claim 1, wherein the functional properties are measured when reed grows to maturity, and the height of reed is 104-112 cm.
3. 3. The method for evaluating the cadmium transport capacity of reed based on plant functional properties according to claim 1, wherein the pore conductance and the transpiration rate of the reed are 8-12 a.m., the 1 st-3 rd leaf of the upper part of the reed is selected for measurement, and the leaf width is 0.9-1.2 cm.
4. 4. The method for evaluating the cadmium transportation capability of reed based on plant functional properties according to claim 1, wherein the base diameter of reed is the diameter of the stalk at the bottom of the plant, and is measured at a position 2-5cm away from soil by using a vernier caliper.

Description

Reed cadmium transport capacity assessment method based on plant functional characters Technical Field The invention belongs to the technical field of soil remediation, and relates to a method for evaluating the cadmium transport capacity of reed based on plant functional properties. Background Heavy metal pollution is increasingly serious, and becomes one of important environmental problems in China and even worldwide, wherein the most serious is cadmium pollution, and how to solve the ecological restoration problem of the heavy metal cadmium polluted soil in China is a difficulty to be solved in China. According to the investigation publication of national soil pollution conditions published by the original environmental protection department and the national resource department in 2014, cadmium is arranged at the 1 st position in 11 kinds of pollutants to be investigated, and the point position exceeding rate reaches 7.0%. Cadmium is also a heavy metal which is monitored in a major part in the act of soil pollution control Programming issued by the national institute of Care in 2016. Among 11 pollutants investigated by national soil environmental standards (GB 15618-2018) in 2018, cadmium with an exceeding standard of agricultural soil pollution risk screening value is also arranged at the 1 st position. The detailed result of soil pollution condition in 2021 Chinese ecological environment Condition publication issued by the ecological environment department of 2021 shows that the main pollutant affecting the soil environment quality of agricultural land is heavy metal, wherein cadmium is the main pollutant. Phytoremediation technology developed based on the absorption and enrichment of heavy metals in polluted environments by specific plants is a new technology for treating heavy metal polluted soil which is internationally developed in recent years, and is attracting attention due to the advantages of environmental friendliness, green, low carbon and the like. At present, some methods for repairing soil cadmium pollution by using specific plants exist, for example, a method for optimally repairing the moderately cadmium-polluted soil by using black nightshade is disclosed in Chinese patent document CN202111314353.5, and the method promotes the growth of black nightshade by additionally fertilizing in the bud period of the black nightshade, so that the enrichment capacity of the black nightshade on the soil is improved, and the aim of repairing the moderately cadmium-polluted soil is fulfilled by harvesting the whole black nightshade in the mature period. Chinese patent document CN202010347575.6 discloses a strengthening repair method for repairing cadmium-polluted soil by using super-enriched plants, wherein the method uses malachite seedlings as the super-enriched plants, and salicylic acid solution as a strengthening agent, so that the cadmium-polluted repair capability of the malachite seedlings is obviously strengthened by the strengthening agent. Among the various methods for remedying the cadmium pollution of soil by utilizing plants disclosed at present, the application of reed is less. Reed (PHRAGMITES AUSTRALIS) is used as a typical wetland plant, has the characteristics of developed root system, abundant strain, strong adaptability and the like, and can transport cadmium to the overground part and then take away cadmium pollution in soil by harvesting the overground part. However, the application of reed in cadmium pollution restoration at present is mainly focused on the aspect of preparing biochar. For example, chinese patent document CN202010300321.9 discloses a reed stem biochar composite material, which is used for repairing soil contaminated by heavy metal cadmium. Chinese patent document CN201910622757.7 discloses a preparation method of multi-site activated and modified reed-triarrhena biochar, which is used for removing heavy metals zinc and cadmium in water and soil. But lacks a method for directly applying reed plants to cadmium pollution remediation. In general, a relatively general method is to calculate the transport coefficient of plants after cadmium treatment is applied, for example, chinese patent document CN201811003842.7 discloses a method for improving the cadmium enrichment coefficient and transport coefficient of ramie, which finds that the enrichment and transport capacity of ramie for harvesting fiber in the mature period of fiber process is higher. But lacks a method of how to evaluate the ability of a plant to transport cadmium without applying cadmium treatment. Therefore, the method for screening the high-transportation reed according to the functional characteristics is developed, not only can the reed strain with strong transportation capability be selected quickly and efficiently, but also the cadmium transportation capability of the reed can be compared only according to the determination of the functional characteristics under the condition that cadmium treat