CN-116273228-B - Microplate assembly with closure function and method for making closure

CN116273228BCN 116273228 BCN116273228 BCN 116273228BCN-116273228-B

Abstract

The application relates to a micropore plate component with a closing function and a manufacturing method of a sealing cover, which comprises a micropore plate component body, wherein the micropore plate component body comprises a micropore plate base, a plurality of glass inner insertion pipes and a plurality of sealing covers, a plurality of well seats which are arranged in geometric patterns are arranged on one side of a substrate of the micropore plate base, gaps are reserved between every two adjacent well seats, a containing well is arranged in the well seat, the sealing cover is embedded in the containing well, the sealing cover is limited in a constraint axial direction of the containing well, a film is coated on a main body of the sealing cover, the glass inner insertion pipes are in plug-and-play elastic connection with the sealing cover after the film is coated, and the sealing cover and the sealing end face part of the bottle mouth are not covered with films.

Inventors

ZHU JIANFENG
Tian hengsheng

Assignees

浙江欧尔赛斯科技有限公司

Dates

Publication Date: 20260512
Application Date: 20230413

Claims (6)

1. A microplate assembly with a closing function is characterized by comprising a microplate assembly body, wherein the microplate assembly body comprises a microplate base, a plurality of glass inner insertion tubes and a plurality of sealing covers, a plurality of well seats which are arranged in a geometric pattern are arranged on one side of a substrate of the microplate base, gaps are formed between every two adjacent well seats, accommodating wells are arranged in the well seats, sealing covers are embedded in the accommodating wells, the sealing covers are limited in the restraining axial direction of the accommodating wells, a film is coated on the outer end face of a sealing cover main body, no film is arranged on the sealing end face part of the sealing cover, the sealing cover is in sealing connection with a bottle opening, the glass inner insertion tubes are in plug-in elastic connection with the sealing cover after the film is coated on the main body, protruding limiting steps are arranged in the accommodating wells, the inner side faces of the limiting steps are H with the bottom of the accommodating wells, the glass inner insertion tubes are made of borosilicate glass, flanges are arranged on one side ends of the glass inner insertion tubes with inner cavities, the inner cavities are blind holes, the opening ends of the inner cavities are consistent with the flanges, the sealing covers are embedded in the positions, the inner cavities of the glass insertion tubes are in the sealing cavities, the inner side of the glass insertion steps are in sealing cover, the sealing covers of the sealing covers and the sealing covers are in sealing body plug-in elastic connection with the flange, and the inner side faces of the limiting steps are in a sealing-in a pressing mode.
2. The microplate assembly of claim 1, wherein the microplate receptacles are 96 well receptacles arranged in a 12 by 8 array.
3. The micro-pore plate assembly with a closing function is characterized in that the sealing cover is made of rubber, a step is arranged on one side of a main body of the sealing cover, the width of the step is L, the width L of the step is smaller than the width H of the inner side face of a limiting step and the bottom of a containing well, the diameter of the step is larger than the diameter of the main body, the diameter of the step is consistent with the diameter of a containing well, a taper hole is formed in the sealing cover, the large diameter direction of the taper hole is consistent with the step, the bottom of the taper hole is communicated with a blind hole, the step of the sealing cover is elastically connected with the containing well, and the step is located inside the limiting step after the sealing cover is connected with a well seat of a micro-pore plate base.
4. The microplate assembly of claim 1, wherein the limiting steps are chamfered on opposite sides of the well, and the bottom of the chamfer is connected to the peripheral wall of the well.
5. The microplate assembly of claim 1, wherein said flange has a chamfer on its inner side which meets the specifications of the inner chamfer of the stop step.
6. The microplate assembly of claim 1, wherein the flange has a diameter corresponding to the diameter of the well.

Description

Microplate assembly with closure function and method for making closure Technical Field The application relates to the technical field of sample processing and storage components, in particular to a microplate assembly with a closing function and a sealing cover production method. Background The growth in medical and pharmaceutical research and diagnostic analysis and testing has created a need for low cost, efficient sample handling equipment and procedures, and automated equipment that can be used to fill and retrieve samples from sample containers. Microplates containing multiple sample wells provide a convenient means of storing samples, and automated equipment can be used to position microplates for sample filling, retrieval and analysis. Despite improvements in sample processing devices, many applications require manual operations, such as preparing sample containers or vials, or coating or uncovering samples. In particular, manual modes of operation are basically employed when the number of samples is insufficient to justify custom automated equipment design and construction. The microplate, glass inner cannula, and vial cap in combination with the chromatographic-based spacer provide a container with multiple closures, stable samples during analysis sample runs, and resealing after sample injection if necessary, but all currently in commercial use employ fully covered PTFE membrane rubber stoppers, with the vial mouth being pressure sealed with the membrane material, with the risk of leakage remaining solid because both the membrane material and glass are rigid materials. In view of the above-described related art, the inventors have recognized a need for a microplate assembly having a closing function and improving sealability to prevent and eliminate leakage risks. Disclosure of Invention In order to solve the technical problems, the application provides a microplate assembly with a closing function and a manufacturing method of a sealing cover, which comprise a microplate assembly body, wherein the microplate assembly body comprises a microplate base, a plurality of glass inner insertion tubes and a plurality of sealing covers, a plurality of well seats which are arranged in geometric patterns are arranged on one side of a substrate of the microplate base, gaps are reserved between every two adjacent well seats, a containing well is arranged in the well, the sealing cover is arranged in the containing well in an embedded manner, the sealing cover is limited in the constraint axial direction of the containing well, a film is coated on a main body of the sealing cover, the glass inner insertion tubes are in plug-and-play elastic connection with the sealing cover after coating the film, and the sealing cover is partially coated with the film, so that the sealing cover has no film on the sealing end face part of the sealing cover, and the sealing cover can be rapidly and easily applied to a plurality of sample containers of the microplate, and the effects of cross contamination and leakage of samples can be reduced. The application provides a microplate assembly with a closing function, which adopts the following technical scheme that the microplate assembly comprises a microplate assembly body, wherein the microplate assembly body comprises a microplate base, a plurality of glass inner cannulas and a plurality of sealing covers; a plurality of well seats arranged in geometric patterns are arranged on one side of a substrate of the micro-pore plate base, gaps are reserved between every two adjacent well seats, and accommodating wells are arranged in the well seats; A sealing cover is arranged in the accommodating well in an embedded mode, and the sealing cover is limited in the constraining axial direction of the accommodating well; and the sealing cover is covered with a film, and the glass inner cannula is connected with the sealing cover after the film is covered in a pulling-inserting type elastic manner. By adopting the technical scheme, the seal can be quickly and easily applied to a plurality of sample containers of the microplate so as to reduce cross contamination of samples, and compared with a plastic microplate well, the seal can improve chemical inertness. Optionally, the microplate base is 96 well seats, and the well seats are arranged in a 12 by 8 array. By adopting the technical scheme, a plurality of independent sample containers can be fully utilized, the operation is convenient, and the stored samples have no hidden trouble of cross contamination. Optionally, a raised limit step is arranged in the accommodating well, and the width between the inner side surface of the limit step and the bottom of the accommodating well is H. By adopting the technical scheme, the limiting step can axially position and restrict the axial limitation of the sealing cover and the glass inner insertion tube after the connection of the accommodating well. Optionally, the sealing cover is made of rubber, a