CN-116286848-B - Application of canna officinalis BEL1-LIKE HOMEODOMAIN transcription factor MaBEL1 in improving cold resistance of fruits

Abstract

The invention relates to the technical field of plant stress resistance, in particular to application of a plantain BEL1-LIKE HOMEODOMAIN transcription factor MaBEL in improving cold resistance of fruits, which creates conditions and a theoretical basis for cultivation of cold-resistant plant varieties and is suitable for large-scale production and application.

Inventors

• LI XUEPING
• ZHU XIAOYANG
• Song Zunyang
• CHEN WEIXIN

Assignees

• 华南农业大学

Dates

Publication Date

20260512

Application Date

20220715

Claims (2)

1. 1. The application of the transcription factor MaBEL1 of the canna sativa BEL1-LIKE HOMEODOMAIN in improving cold resistance of canna sativa fruits is disclosed, and the sequence of MaBEL1 is shown as SEQ ID NO. 1.
2. 2. The application of a protein sequence numbered by a transcription factor MaBEL1 of the plantain BEL1-LIKE HOMEODOMAIN in improving cold resistance of plantain fruits is disclosed, and the protein sequence is shown as SEQ ID NO. 2.

Description

Application of canna officinalis BEL1-LIKE HOMEODOMAIN transcription factor MaBEL1 in improving cold resistance of fruits Technical Field The invention relates to the technical field of plant stress resistance, in particular to application of a canna BEL1-LIKE HOMEODOMAIN transcription factor MaBEL1 in improving cold resistance of fruits. Background The banana (Musa ABB PISANG AWAK) belongs to the Musaceae, is a novel characteristic banana in recent years, has thin and thick skin, fresh and sweet taste, rich nutrition and good disease resistance, is deeply favored by consumers, is widely planted in the southern area of China, has the yield which is about 20 percent of the total yield of the banana, increases the facility cultivation area year by year, and is one of the most important fruit crops in China. The banana belongs to tropical and subtropical fruits, is sensitive to low temperature, and can easily cause cold damage to the fruits if the growth temperature is lower than 11 ℃ or the storage temperature is lower than 11 ℃, so that the quality and commodity value are reduced. With the increase of global extreme climate events, stress resistance of crops is more and more of an important concern. Weather below 0 ℃ often occurs in winter in southern areas of China, and particularly, the air temperature is lower in northern areas of Guangdong in China, so that cold damage symptoms of the plantain are more likely to occur, and the quality and commercial value of the plantain are seriously affected. Therefore, in order to cope with the influence of low-temperature stress on the banana fruits, it is particularly important to cultivate low-temperature-resistant banana varieties. At present, the research on stress resistance mechanisms of crops for resisting low temperature is relatively backward, particularly in the aspect of cold resistance research of the plantains, not only obvious breakthroughs are not obtained in measures and means for solving the cold resistance problem of the plantains, but also the variety of the plantains capable of resisting low temperature is not found, and great trouble is brought to the production of the plantains, so that the method for analyzing the cold-resistant key genes of the crops such as the plantains and the like, analyzing the mechanism of fruits for resisting low temperature is very important, and cultivating the low-temperature-resistant crops. Disclosure of Invention The invention aims to avoid the defects in the prior art and provide the application of the plantain BEL1-LIKE HOMEODOMAIN transcription factor MaBEL1 in improving the cold resistance of fruits, which can obviously improve the cold resistance of transgenic fruits. In order to achieve the above purpose, the present invention provides the following technical solutions: The application of the transcription factor MaBEL1 of the canna sativa BEL1-LIKE HOMEODOMAIN in improving the cold resistance of fruits is provided, and the sequence of MaBEL1 is shown as SEQ ID NO. 1. In some embodiments, the fruit is canna. Also provided is the application of the protein sequence numbered by the transcription factor MaBEL1 of the plantain BEL1-LIKE HOMEODOMAIN in improving the cold resistance of plants, wherein the protein sequence is shown as SEQ ID NO. 2. In some embodiments, the fruit is canna. According to the technical scheme, the BEL1-LIKE HOMEODOMAIN transcription factor MaBEL1 is cloned from the plantain through analysis and design of specific primers according to the genome sequence of the plantain, the plantain fruits are transiently overexpressed, the cold resistance of the transgenic fruits is remarkably improved, and the research results provide a beneficial method and thought for creating the plantain cold-resistant material. BEL1-LIKE HOMEODOMAIN transcription factor MaBEL1 (hereinafter abbreviated as MaBEL 1) has a length of 2004bp, codes 667 amino acid, and has the highest homology with MaBEL-like 4 of banana and SlBEL-like 4 of tomato. The MaBEL gene sequence is obtained by using cDNA of Guangdong No. 1 as a template, using primers MaBEL-F and MaBEL-R, and performing clone sequencing. The BEL1-LIKE HOMEODOMAIN protein sequence coded by MaBEL gene has relative molecular weight of 57.65kDa, isoelectric point pI of 7.13 and alkalescence. The overexpressed banana fruit MaBEL1 can induce the proportion of unsaturated fatty acid and flavonoid content of the banana fruit. The application of the transcription factor MaBEL1 of the plantain BEL1-LIKE HOMEODOMAIN in improving the cold resistance of the plantain is that the transcription factor MaBEL1 of the plantain BEL1-LIKE HOMEODOMAIN is cloned from the plantain by designing a specific primer according to the genome sequence of the plantain, and the gene has the length of 2004bp and codes 667 amino acids. Bioinformatics analysis shows that the homology with MaBEL-like 4 of banana and SlBEL-like 4 of tomato is highest, the banana is positioned in cell nucleus, the