CN-116322353-B - Anticoccidial composition comprising ginkgo leaf and use thereof

CN116322353BCN 116322353 BCN116322353 BCN 116322353BCN-116322353-B

Abstract

The application relates to an anticoccidial composition containing ginkgo leaf and application thereof. According to an embodiment, the composition comprising ginkgo leaf has an excellent effect of directly killing sporophytes which can induce coccidiosis, inhibiting penetration of sporophytes into cells, and/or inhibiting proliferation of sporophytes in cells, and has an excellent effect of preventing, improving and treating coccidiosis in vivo.

Inventors

Sun Guilie
Pu Minwo
LI JINGMIN
Jin Huizai

Assignees

CJ第一制糖株式会社

Dates

Publication Date: 20260508
Application Date: 20210924
Priority Date: 20200925

Claims (7)

1. The use of at least one compound selected from the group consisting of bilobalide A, bilobalide B, bilobalide C, bilobalide and salts thereof as an active ingredient for the preparation of a composition for the prevention, alleviation or treatment of coccidiosis, Wherein ginkgolide A or a salt thereof is contained in the composition at a concentration of 31ppm or more; ginkgolide B or its salt is contained in the composition at a concentration of 125ppm or more; ginkgolide C or its salt is contained in the composition at a concentration of 125ppm or more; The bilobalide or its salt is contained in the composition at a concentration of 63ppm or more, and Wherein, the the coccidiosis is formed by Eimeria genus (Eimeria sp.) protozoan induced.
2. The use according to claim 1, wherein the compound further comprises at least one selected from the group consisting of quercetin, kaempferol and salts thereof as an active ingredient.
3. The use according to claim 1, wherein the prevention, alleviation or treatment of coccidiosis is at least one selected from the group consisting of (1) to (4): (1) Reducing at least one selected from the group consisting of lesion score, fecal oocyst excretion, and mortality; (2) Inhibiting weight loss due to coccidiosis; (3) Increasing anticoccidial index (ACI), and (4) Reduce cell invasion of eimeria protozoa, proliferation of protozoa in cells, or both.
4. The use of at least one compound selected from the group consisting of bilobalide A, bilobalide B, bilobalide C, bilobalide and salts thereof as an active ingredient for the preparation of a pharmaceutical composition for the prevention or treatment of coccidiosis, Wherein ginkgolide A or a salt thereof is contained in the composition at a concentration of 31ppm or more; ginkgolide B or its salt is contained in the composition at a concentration of 125ppm or more; ginkgolide C or its salt is contained in the composition at a concentration of 125ppm or more; the bilobalide or its salt is contained in the composition at a concentration of 63ppm or more, and Wherein, the the coccidiosis is formed by Eimeria genus (Eimeria sp.) protozoan induced.
5. The use according to claim 4, wherein the compound further comprises at least one selected from the group consisting of quercetin, kaempferol and salts thereof as an active ingredient.
6. Use of at least one compound selected from the group consisting of bilobalide A, bilobalide B, bilobalide C, bilobalide and salts thereof as an active ingredient for the preparation of an antiprotozoal composition against Eimeria protozoa, Wherein ginkgolide A or a salt thereof is contained in the composition at a concentration of 31ppm or more; ginkgolide B or its salt is contained in the composition at a concentration of 125ppm or more; ginkgolide C or its salt is contained in the composition at a concentration of 125ppm or more; The bilobalide or salt thereof is contained in the composition at a concentration of 63ppm or more.
7. The use according to claim 6, wherein the compound further comprises at least one selected from the group consisting of quercetin, kaempferol and salts thereof as an active ingredient.

Description

Anticoccidial composition comprising ginkgo leaf and use thereof Cross Reference to Related Applications The present application claims the benefit of priority based on korean patent application No. 10-2020-0125274 filed on 25 th 9 of 2020, and the entire contents of the disclosures of the corresponding korean patent application documents are incorporated as part of the present specification. Technical Field The application relates to an anticoccidial composition containing ginkgo leaf and application thereof. Background Coccidiosis (coccidiosis) is an intestinal related disease caused by protozoan parasites belonging to the genus Eimeria (Eimeria) belonging to the genus apicomplexa (phylum apicomplexan), and when infected with coccidiosis, symptoms of digestive system disorders, diarrhea and weight loss occur, and moreover, death of livestock occurs. Thus, it has a significant economic impact on farms worldwide (Williams RB., partition model (Acompartmentalised model for the estimation of the cost of coccidiosis to theworld's chicken production industry),Int J Parasitol,1999 for estimating coccidiosis costs in chicken industry worldwide; 29 (8): 1209-1229). Over the past few years, many researchers have developed anticoccidial agents, such as ionophores or chemically synthesized compounds, that can prevent the formation of oocyst cell walls or asexual and sexual proliferation of protozoans as therapeutics for the treatment of coccidiosis. However, side effects such as the emergence of resistant protozoa occur due to long-term use of shuttle procedures that alternately treat emerging ionophores and chemically synthesized compounds, and the like. In particular, since the accumulation of antibiotics in animals is a serious problem due to misuse and abuse of antibiotics, since humans consume antibiotics through meats, many countries around the world are banning the administration of antibiotics due to the problem of antibiotic residues in animal products. Accordingly, there is an urgent need to develop and study alternatives to conventional anticoccidial agents that exhibit side effects such as the appearance of drug-resistant strains and internal residues. Prior Art Patent literature (Patent document 1) U.S. patent publication No. 2008-0160000 Disclosure of Invention Technical problem An embodiment of the present application provides a feed composition for preventing or alleviating coccidiosis, comprising ginkgo leaves. In another embodiment of the present application, there is provided a pharmaceutical composition for preventing or treating coccidiosis, comprising at least one selected from the group consisting of bilobalide a, bilobalide B, bilobalide C, quercetin, kaempferol, bilobalide and pharmaceutically acceptable salts thereof as an active ingredient. Other embodiments of the present application provide a pharmaceutical composition for preventing or treating coccidiosis, comprising ginkgo leaf. Another embodiment of the present invention provides an antiprotozoal composition against Eimeria sp, comprising at least one selected from the group consisting of bilobalide a, bilobalide B, bilobalide C, quercetin, kaempferol, bilobalide and a salt thereof as an active ingredient. Other embodiments of the present application provide an antiprotozoal composition against eimeria protozoa comprising ginkgo leaf. Other embodiments of the application provide a method of preventing, alleviating or treating coccidiosis comprising the step of administering the composition to an animal other than a human. Other embodiments of the present application provide for the use of at least one selected from the group consisting of bilobalide A, bilobalide B, bilobalide C, quercetin, kaempferol, bilobalide and salts thereof in the manufacture of a composition (e.g., a pharmaceutical composition) or in the manufacture of an antiprotozoal composition for the prevention, alleviation and/or treatment of coccidiosis, for the prevention, alleviation and/or treatment of coccidiosis of at least one selected from the group consisting of bilobalide A, bilobalide B, bilobalide C, quercetin, kaempferol, bilobalide and salts thereof, and/or for the prevention, alleviation and/or treatment of coccidiosis of at least one selected from the group consisting of bilobalide A, bilobalide B, kaempferol and salts thereof for the antiprotozoal (e.g., killing eimeria protozoa, and/or inhibiting the cellular invasion and/or proliferation of eimeria protozoa. Other embodiments of the present application provide a use of ginkgo leaf for preparing a composition (e.g., a feed composition, a pharmaceutical composition) for preventing, alleviating and/or treating coccidiosis or preparing an antiprotozoal composition, a use of ginkgo leaf for preventing, alleviating and/or treating coccidiosis, and/or a use of ginkgo leaf for combating eimeria protozoa (e.g., killing eimeria protozoa, and/or inhibiting cell invasion and/or proliferation of eimeria protozoa).