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CN-116327818-A - Method for extracting flavonoid aglycone in flos Chrysanthemi, extract obtained by the method, and anti-inflammatory pharmaceutical composition

CN116327818ACN 116327818 ACN116327818 ACN 116327818ACN-116327818-A

Abstract

A method for extracting flavonoid aglycone (flavone aglycones) from flos Chrysanthemi is provided. The method comprises the following steps: (a) Soaking flos Chrysanthemi in water or water solution for 3.5 hr to obtain soaked sample; and (b) adding an extraction solvent to the soaked sample to perform an extraction procedure for 5-60 minutes to obtain an extract. The Hangzhou chrysanthemum raw material comprises at least one of the following components of Hangzhou chrysanthemum: whole plant, root, stem, leaf and flower.

Inventors

  • YAO XINRAN
  • CHEN YUWEN
  • LYU JUXUN
  • PAN YIHONG
  • CHEN MINYIN
  • YANG ZONGLIN
  • WU JIAJIA

Assignees

  • IND TECH RES INST

Dates

Publication Date
20230627
Application Date
20211223
Priority Date
20211223

Claims (20)

  1. 1. A method for extracting flavonoid aglycone (flavone aglycones) in chrysanthemum morifolium ramat, comprising: (a) Soaking flos Chrysanthemi in water or water solution for 3.5 hr to obtain soaked sample, wherein the method comprises the steps of The Hangzhou chrysanthemum raw material comprises at least one of the following components of Hangzhou chrysanthemum: whole plants, roots, stems, leaves and flowers, the weight ratio of the chrysanthemum raw material to the water or the aqueous solution is 1:10-35, the soaking procedure is carried out at 20 to 70 c, the water or aqueous solution has a pH of 3.0 to 9.5, and the soaking sample comprises soaked chrysanthemum raw materials and soaking liquid; and (b) Adding an extraction solvent into the soaked sample to perform an extraction procedure for 5-60 minutes to obtain an extract, wherein the method comprises the steps of The weight ratio of the chrysanthemum raw material to the extraction solvent is 1:10-35, the extraction solvent comprises methanol, ethanol or ethyl acetate, the extraction procedure is carried out at 15 to 50 ℃, and the extract contains flavonoid aglycone.
  2. 2. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 1, wherein said Hangzhou chrysanthemum material is pre-treated.
  3. 3. The method of extracting flavonoid aglycone from chrysanthemum morifolium ramat of claim 2, wherein said pretreatment comprises at least one of: drying; and and (5) crushing.
  4. 4. The method of extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 1, further comprising removing said infusion solution from said infusion sample between step (a) and step (b).
  5. 5. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 1, wherein said soaking procedure is performed for 5 to 96 hours.
  6. 6. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 1, wherein the weight ratio of said Hangzhou chrysanthemum raw material to said water or aqueous solution is 1:15-30.
  7. 7. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 1, wherein said soaking procedure is performed at a temperature of between room temperature and 70 ℃.
  8. 8. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 1, wherein said water or aqueous solution has a pH of 4.0 to 9.5.
  9. 9. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 1, wherein said extraction procedure is performed for 10 to 40 minutes.
  10. 10. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 1, wherein the weight ratio of said Hangzhou chrysanthemum raw material to said extraction solvent is 1:15-30.
  11. 11. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 1, wherein said extraction procedure is performed at a temperature of between room temperature and 40 ℃.
  12. 12. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 1, wherein the concentration of said ethanol is 50% to 95%.
  13. 13. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 1, wherein said extraction procedure is performed under shaking or stirring.
  14. 14. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 13, wherein said shaking comprises ultrasonic shaking or rotational shaking.
  15. 15. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 13, wherein said means of agitation includes stirring with a paddle stirrer or an electromagnetic stirrer.
  16. 16. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 1, wherein said Hangzhou chrysanthemum material is flowers of said Hangzhou chrysanthemum.
  17. 17. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 16, wherein said soaking procedure is performed for 5 to 72 hours, the weight ratio of said Hangzhou chrysanthemum raw material to said water or aqueous solution is 1:15-20, and said soaking procedure is performed at room temperature to 50 ℃, said water or aqueous solution has a pH of 5.0 to 9.2, and said extraction solvent is ethanol.
  18. 18. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 17, wherein said method for extracting Hang Juhuang ketoaglycone has a flavonoid aglycone extraction rate of 2-30mg flavonoid aglycone per gram Hangzhou chrysanthemum raw material.
  19. 19. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 1, wherein said Hangzhou chrysanthemum material is stems and leaves of said Hangzhou chrysanthemum.
  20. 20. The method for extracting flavonoid aglycone from Hangzhou chrysanthemum according to claim 19, wherein said soaking procedure is performed for 5 to 72 hours, the weight ratio of said Hangzhou chrysanthemum raw material to said water or aqueous solution is 1:15-20, and said soaking procedure is performed at room temperature to 50 ℃, said water or aqueous solution has a pH of 5.0 to 9.2, and said extraction solvent is ethanol.

Description

Method for extracting flavonoid aglycone in flos Chrysanthemi, extract obtained by the method, and anti-inflammatory pharmaceutical composition Technical Field The present disclosure relates to a method of extracting flavonoid aglycones in Hangzhou chrysanthemum, extracts obtained by the method and anti-inflammatory pharmaceutical compositions. Background The Hangzhou chrysanthemum belongs to the Asteraceae (Asteraceae) plant, is a perennial herb plant and is mainly produced in Taiwan area, china continent and the like. The flower heads of the Hangzhou chrysanthemum can be used as beverage after being dried, and the flower heads also belong to traditional medicinal plants. Modern pharmacological studies show that chrysanthemum morifolium ramat has the functions of antioxidation, anti-inflammation, antivirus, anti-tumor, neuroprotection and cardiovascular protection. Flos Chrysanthemi contains chrysanthemi glycoside, amino acids, flavonoids, vitamins and microelements. The chemical components of the Hangzhou chrysanthemum are complex, wherein flavonoid compounds, triterpene compounds and volatile oil are main active ingredients, however, the chemical components of the Hangzhou chrysanthemum from different sources have different compositions and contents due to different production places and varieties. Flavonoid compounds are secondary metabolites synthesized by plants and play important roles in the growth, development, flowering, fruiting, antibacterial and disease prevention of plants, the flavonoid compounds are usually combined with sugar in plant bodies to form glycosides, and a small part of flavonoid compounds exist in a free form (aglycones). Previous studies have shown that the flavonoids of free aglycone have better anti-inflammatory activity than the glycoside derivatives. In recent years, many researchers have tried to hydrolyze flavonoid glycoside derivatives into free aglycone by means of enzyme hydrolysis or microbial fermentation, however, the extraction cost is greatly increased by adding enzyme or microbial fermentation. Therefore, there is a need for a novel way of extracting flavonoid aglycones that is effective in extracting flavonoid aglycones from chrysanthemum without any enzyme addition and/or microbial treatment. Disclosure of Invention The present disclosure provides a method of extracting flavonoid aglycone (flavone aglycones) in chrysanthemum morifolium, comprising: (a) Soaking flos Chrysanthemi in water or water solution for 3.5 hr to obtain soaked sample; and (b) adding an extraction solvent to the soaked sample to perform an extraction procedure for 5-60 minutes to obtain an extract. The Hangzhou chrysanthemum raw material comprises at least one of the following components of Hangzhou chrysanthemum: the weight ratio of the whole plant, root, stem, leaf and flower, the chrysanthemum raw material and the water or water solution is 1:10-35, the soaking procedure is carried out at 20-70 ℃, the water or water solution has a pH value of 3.0-9.5, and the soaked sample comprises the soaked chrysanthemum raw material and soaking solution. The weight ratio of the chrysanthemum raw material to the extraction solvent is 1:10-35, the extraction solvent comprises methanol, ethanol or ethyl acetate, the extraction process is carried out at 15-50 ℃, and the extract contains flavonoid aglycone. The present disclosure also provides a chrysanthemum morifolium extract obtained by the method for extracting flavonoid aglycone in chrysanthemum morifolium. The present disclosure also provides an anti-inflammatory pharmaceutical composition comprising: the chrysanthemum extract is obtained by the method for extracting flavonoid aglycone in chrysanthemum; and pharmaceutically acceptable carriers or salts. The foregoing and other objects, features and advantages of the invention will be apparent from the following more particular description of preferred embodiments, as illustrated in the accompanying drawings in which: drawings FIG. 1 shows a high performance liquid chromatography of extracts of flowers of Hangzhou chrysanthemum with different soaking times. Fig. 2 shows the extraction amounts (extraction rates) of apigenin and luteolin of the soaking solution and the extraction solution obtained at different soaking times (room temperature) of flowers of chrysanthemum morifolium. Fig. 3A shows the extraction amount (extraction rate) per unit of apigenin (apigenin) of the extract obtained at different pH values and different soaking room temperatures for flowers of chrysanthemum morifolium. FW: flower weight of Hangzhou chrysanthemum. Fig. 3B shows the extraction amount (extraction rate) per unit of medicinal material of luteolin (luteolin) of the extract obtained from flowers of chrysanthemum morifolium at different pH values and different soaking room temperatures. FW: flower weight of Hangzhou chrysanthemum. FIG. 4 shows the HPLC of the extracts obtained from the stems and leaves of Hangzhou chrysa