CN-116380863-B - Polysaccharide detection method based on metal-organic framework blended composite film Raman spectrum

Abstract

The invention discloses a polysaccharide detection method based on a metal-organic framework blended composite film Raman spectrum, which comprises the following steps of S1, preparing a reaction, namely storing a product to be detected in a glass vessel, blocking an opening of the glass vessel to isolate an inner cavity of the glass vessel from an external environment, and storing the glass vessel, S2, generating the reaction, namely opening the glass vessel in S1, adding a sulfhydryl Raman dye and a halogen high-valence oxidant into the glass vessel, and further reacting the product with the sulfhydryl Raman dye and the halogen high-valence oxidant to generate a polysaccharide oxidation product. According to the polysaccharide detection method based on the metal-organic framework blended composite film Raman spectrum, the advantages of enzyme reaction, complex chemical synthesis, labeling and the like are not needed, and the metal-organic framework and the polymer are blended, so that the surface aggregation of gold nanoparticles is prevented, the surface enhanced Raman scattering intensity is improved, and the detection stability and sensitivity are improved.

Inventors

• CAO HUABIN
• LAN DUAN
• LAI CHUNLIAN
• DU QINSHENG
• LI SHUIZHEN

Assignees

• 龙岩嘉麒生物科技有限公司

Dates

Publication Date

20260505

Application Date

20221116

Claims (9)

1. 1. A polysaccharide detection method based on a metal-organic framework blended composite film Raman spectrum comprises the following steps of: S1, preparing reaction, namely storing a product to be detected in a glass vessel, and plugging an opening of the glass vessel to isolate an inner cavity of the glass vessel from an external environment; S2, the glassware in the step S1 is opened, and a sulfhydryl Raman dye and a halogen high-valence oxidant are added into the glassware to further react the product with the sulfhydryl Raman dye and the halogen high-valence oxidant to generate a polysaccharide oxidation product; S3, preparing detection, namely dripping the reaction product sucked in the S2 by a dropper onto the surfaces of the gold nanoparticles on the metal-organic framework blended composite film; S4, on-site detection, namely setting parameters of Raman spectrum equipment, and blending the surface enhanced Raman scattering technology of the composite film with the metal organic framework in the S3 to obtain the polysaccharide content in the functional plant extract; S5, recording information, namely placing the sample remained after detection into a glassware, attaching label paper for recording information on the surface of the glassware, and plugging the opening of the glassware to isolate the inner cavity of the glassware from the external environment for storage.
2. 2. The method for detecting polysaccharide based on metal-organic framework blended composite film Raman spectrum of claim 1, wherein in S1, the prepared product is 25 mg.
3. 3. The polysaccharide detection method based on the metal-organic framework blended composite film Raman spectrum, which is disclosed in claim 1, is characterized in that glassware in S1 and S5 is placed in a light-proof place for storage.
4. 4. The polysaccharide detection method based on the metal-organic framework blended composite film Raman spectrum, which is disclosed in claim 1, is characterized in that in S2, sulfhydryl Raman dye and halogen high-valence oxidant are added into the interior of a glassware and then slightly shaken.
5. 5. The polysaccharide detection method based on the metal-organic framework blended composite film Raman spectrum, which is disclosed in claim 1, is characterized in that the adopted glassware in S1 and S5 is colorless and sterile glassware.
6. 6. The method for detecting polysaccharide based on the metal-organic framework blended composite film Raman spectrum, which is characterized in that in the step S5, the information recorded on the label paper comprises the sample type, the sample name, the preparation time, the storage time, the detection time and the polysaccharide detection result.
7. 7. The polysaccharide detection method based on the metal-organic framework blended composite film Raman spectrum, which is disclosed in claim 1, is characterized in that in S5, the spacing between a plurality of storage glassware is 5-10 cm.
8. 8. The polysaccharide detection method based on the metal-organic framework blended composite film Raman spectrum, which is disclosed in claim 1, is characterized in that in S1 and S5, sterile silica gel plugging blocks are adopted to plug glassware.
9. 9. The polysaccharide detection method based on the metal-organic framework blended composite film Raman spectrum, which is disclosed in claim 1, is characterized in that in the step S3, the dropper is a disposable plastic dropper.

Description

Polysaccharide detection method based on metal-organic framework blended composite film Raman spectrum Technical Field The invention relates to the technical field of polysaccharide detection, in particular to a polysaccharide detection method based on a metal-organic framework blended composite film Raman spectrum. Background The polysaccharide lacks obvious ultraviolet or fluorescence spectrum, and has weak characteristic spectral line signal and is difficult to directly detect. At present, laboratories mainly rely on large-scale instruments (such as chromatographic instruments) and detection of hydrolyzed monosaccharides, the detection period is long, the actual polysaccharide composition is difficult to directly reflect, the polysaccharide component is difficult to realize on-site monitoring, the polysaccharide is the main component of plants, and many polysaccharides have important pharmacological activities, so that the polysaccharide is used as quality index of many traditional Chinese medicines, and the polysaccharide content monitoring is needed in the pharmaceutical process of the traditional Chinese medicines. For example, the radix codonopsis pilosulae and rhizoma polygonati contain high content of polysaccharide, and the radix codonopsis pilosulae polysaccharide and the rhizoma polygonati polysaccharide have the functions of antioxidation, myocardial protection, neuroprotection, immunoregulation and the like, are medicinal components of radix codonopsis pilosulae and rhizoma polygonati, and are usually measured by adopting an ultraviolet spectrophotometry based on a phenol sulfuric acid method or an anthrone sulfuric acid method at present, so that the method is tedious and time-consuming, and cannot meet the requirement of rapid analysis in pharmaceutical process monitoring. Moreover, the method requires the use of a large amount of strong corrosive concentrated sulfuric acid, which has potential safety hazards. The process analysis technology (PAT guide) published by the FDA in 2004 has promoted the application of PAT technology in the pharmaceutical process of traditional Chinese medicine. PAT techniques based on spectroscopic analysis methods, such as near infrared spectroscopic analysis methods and raman spectroscopic analysis methods, can provide real-time, economical, non-destructive determination of critical quality parameters in the manufacturing process. However, the signal of water in the near infrared spectrum is strong, so that when the near infrared spectrum is used for measuring chemical components in the aqueous solution, the near infrared absorption signal of water can mask the signals of other components, which is unfavorable for measuring the components, and the signal of water in the Raman spectrum is weak, so that the Raman spectrum is suitable for measuring the content of the chemical components in the aqueous solution. At present, raman spectrum measuring methods for monosaccharide and disaccharide in products such as honey, beverage and the like are available in the literature, but no raman spectrum analysis method for polysaccharide measurement is reported. At present, the polysaccharide in the plant extract is detected by performing biological or chemical reaction treatment on the extract to obtain the polysaccharide content, wherein the polysaccharide content mainly comprises an HPLC method, an ELISA method, a dinitrosalicylic acid method, a phenol-sulfuric acid method and the like, the existing technologies such as the HPLC method, the ELISA method, the dinitrosalicylic acid method, the phenol-sulfuric acid method and the like are developed and mature, the precision of an HPLC detection device is higher, the method takes too long time and is troublesome in pretreatment, the lowest detection limit of the ELISA detection method is lower, the sensitivity is high, the detection steps of the method are complex, multiple times of washing are needed, the detection steps of the dinitrosalicylic acid method and the phenol-sulfuric acid method are complex, the traditional detection method cannot react the components in the fermentation liquor in real time, and the hysteresis of the detection result is common. Disclosure of Invention Aiming at the defects of the prior art, the invention provides a polysaccharide detection method based on a metal-organic framework blended composite film Raman spectrum, which solves the problem that the traditional detection method cannot react components in fermentation liquor in real time and the detection result generally has hysteresis. The polysaccharide detection method based on the metal-organic framework blended composite film Raman spectrum comprises the following steps of S1, preparing reaction, namely storing a product to be detected in a glass vessel, blocking an opening of the glass vessel, and isolating an inner cavity of the glass vessel from an external environment; S2, the glassware in the step S1 is opened, and a sulfhydryl Rama