CN-116435164-A - Capillary electrospray ion source device and mass spectrometer thereof

CN116435164ACN 116435164 ACN116435164 ACN 116435164ACN-116435164-A

Abstract

The invention provides a capillary electrospray ion source device, which comprises a shell which is communicated up and down, wherein a pressing sleeve, a cone electrode and a clamp holder which can move up and down are sequentially arranged in the shell from top to bottom; the pressure sleeve protrudes out of the shell and internally provided with a sampling capillary, the bottom of the pressure sleeve is connected with the conical electrode, the sampling capillary is abutted to the upper end of the conical electrode, a conical hole is formed in the conical electrode, the lower end of the sampling capillary is embedded with a spray capillary, the spray capillary penetrates through the clamp holder through the conical hole of the conical electrode and is clamped by the clamp holder, the conical electrode is arranged at one end of the clamp holder, the other end of the clamp holder is provided with a clamping jaw for clamping the spray capillary, and the pressure sleeve and the clamp holder are respectively provided with a rebound piece. The device provided by the invention realizes the integrated operation of spontaneous sample collection, sample transmission and ionization by utilizing capillary action, and has the advantages of simple structure and convenience in operation.

Inventors

ZHAI YANBING
XU WEI
LIANG QIONG

Assignees

BEIJING INSTITUTE TECH
KUNSHAN NIEER PREC INSTRUMENT CO LTD

Dates

Publication Date: 20230714
Application Date: 20221009
Priority Date: 20221009

Claims (8)

1. The capillary electrospray ion source device is characterized by comprising a shell which is communicated up and down, wherein a pressing sleeve, a cone electrode and a clamp holder which can move up and down are sequentially arranged in the shell from top to bottom; the pressing sleeve protrudes out of the shell and is internally provided with a sampling capillary, the bottom of the pressing sleeve is connected with the cone electrode, the sampling capillary is abutted to the upper end of the cone electrode, a cone hole is formed in the cone electrode, a spray capillary is embedded in the lower end of the sampling capillary, the spray capillary penetrates through the clamp holder through the cone hole of the cone electrode and is clamped by the clamp holder, the cone electrode is arranged at one end of the clamp holder, the other end of the clamp holder is provided with a clamping jaw for clamping the spray capillary, the pressing sleeve and the clamp holder are respectively provided with a rebound piece, and when the pressing sleeve is pressed, the pressing sleeve presses the cone electrode and pushes the clamp holder to loosen the spray capillary; when the pressing sleeve is loosened, the pressing sleeve, the conical electrode and the clamp holder are reset under the driving of the rebound piece, and the spray capillary is clamped.
2. The capillary electrospray ion source device according to claim 1, wherein the rebound member of the pressing sleeve comprises a spring and a rebound limit, the rebound limit is formed by matching a convex object arranged on the outer wall of the pressing sleeve with a shoulder surface of the cavity of the inner wall of the shell, and the spring is sleeved between the convex object arranged on the outer wall of the pressing sleeve and the cone electrode.
3. The capillary electrospray ion source device according to claim 1 or 2, wherein the holder comprises a holding rod capable of internally holding the spray capillary and a rebound member of the holder, one end of the holding rod is connected with the cone electrode, the other end of the holding rod is provided with a clamping jaw, the rebound member of the holder comprises a convex object on the outer wall of the holding rod, a fixing member and a spring sleeved between the convex object on the outer wall of the holding rod and the fixing member, the fixing member is fixed in a cavity at one end of the shell and sleeved outside one end of the holding rod with the clamping jaw, a cavity capable of allowing the holding rod to move up and down is formed between the fixing member and the holding rod, the holding rod moves downwards, the clamping jaw is separated from the fixing member, the clamped spray capillary is loosened, and when the holding rod is reset, the clamping jaw retracts into the fixing member to clamp the spray capillary.
4. The capillary electrospray ion source device according to claim 1, wherein the housing comprises an upper housing and a lower housing, the upper housing and the lower housing being connected by a threaded connection or by a snap-fit connection.
5. The capillary electrospray ion source device according to claim 1, wherein the sampling capillary is a capillary having an outer diameter of 0.69-2mm and an inner diameter of 590-1900 μm.
6. The capillary electrospray ion source device according to claim 1, wherein the spray capillary is a capillary having an outer diameter of 150-360 μm and an inner diameter of 75-150 μm.
7. The capillary electrospray ion source device according to claim 1, wherein the spray capillary upper end is embedded 1-3mm into the sampling capillary.
8. A mass spectrometer comprising a capillary electrospray ion source apparatus according to any one of claims 1 to 7.

Description

Capillary electrospray ion source device and mass spectrometer thereof Technical Field The invention relates to the technical field of mass spectrometry detection and analysis, in particular to a capillary electrospray ion source device and a mass spectrometer thereof. Background The ion source is an important component of a mass spectrometer, and has the main functions of ionizing a compound to be detected by means of physical or chemical reaction and the like by utilizing the physical and chemical principles of light, heat, electricity and the like, so that the compound to be detected enters a mass analyzer to be subjected to subsequent operations such as separation, detection and the like. The development of the ion source drives the development of the mass spectrum, and meanwhile, the development of the mass spectrum also provides new requirements for the ion source. Electrospray ion sources (Electrospray Ionization, ESI) are among the most widely used ion sources today to enable ionization analysis of non-volatile samples. As a soft ionization technique that does not generate molecular fragments, ESI can directly measure thermally labile compounds and generate multi-charged ions, and has unique advantages in metabonomics, proteomics, and other studies. However, conventional commercial electrospray ion sources are typically bulky, complex in structure, expensive to sell, and require additional gas cylinders and heating devices to provide atomizing assist gas to enhance desolvation and ionization efficiency, which greatly limit the coupling of ESI to a small mass spectrometer and its use for rapid detection. In addition, the large spray flow rates of conventional ESI also result in ionization efficiencies that are generally low. The Nano-liter electrospray ion source (Nano-ESI) is a mass spectrometry technology developed after ESI, which improves ionization efficiency of the electrospray ion source by reducing spray flow rate on one hand, and has simpler structure on the other hand, and no auxiliary equipment such as high-temperature carrier gas and the like is needed. However, the spray tip (inner diameter to μm) of Nano-ESI generally requires a special capillary drawing instrument for drawing, and the equipment cost is high, and the tip of Nano-ESI is extremely fragile and clogged due to the extremely small pore size, which affects the normal analysis of the sample. Therefore, based on the electrospray ionization principle, it is necessary to develop a high-sensitivity electrospray ion source which is simple in structure, small and portable and low in cost, so that the electrospray ion source can be combined with different mass spectrum detection instruments, especially a small mass spectrometer, and is used for rapid analysis of different scenes. Disclosure of Invention In order to solve the problems, the invention provides a capillary electrospray ion source device which has a simple structure and low cost, and simultaneously realizes the integrated operation of spontaneous sample collection, sample transmission and ionization, and can be coupled and used with a plurality of mass spectrographs including a small mass spectrometer. It is another object of the present invention to provide a mass spectrometer comprising the capillary electrospray ion source apparatus. In order to achieve the technical aim, the capillary electrospray ion source device comprises a shell which is communicated up and down, wherein a pressing sleeve, a cone electrode and a clamp holder which can move up and down are sequentially arranged in the shell from top to bottom; the pressing sleeve protrudes out of the shell and is internally provided with a sampling capillary, the bottom of the pressing sleeve is connected with the cone electrode, the sampling capillary is abutted to the upper end of the cone electrode, a cone hole is formed in the cone electrode, a spray capillary is embedded in the lower end of the sampling capillary, the spray capillary penetrates through the clamp holder through the cone hole of the cone electrode and is clamped by the clamp holder, the cone electrode is arranged at one end of the clamp holder, the other end of the clamp holder is provided with a clamping jaw for clamping the spray capillary, the pressing sleeve and the clamp holder are respectively provided with a rebound piece, and when the pressing sleeve is pressed, the pressing sleeve presses the cone electrode and pushes the clamp holder to loosen the spray capillary; when the pressing sleeve is loosened, the pressing sleeve, the conical electrode and the clamp holder are reset under the driving of the rebound piece, and the spray capillary is clamped. Further, the rebound piece of the pressing sleeve comprises a spring and rebound limit, the rebound limit is formed by matching a convex object arranged on the outer wall of the pressing sleeve with a shoulder surface of the cavity of the inner wall of the shell, and the spring is sleeved bet