Search

CN-116445291-B - Acer maple bioluminescence strain S-LWZ20190614-6 and application thereof in degradation of lignin and bisphenol S

CN116445291BCN 116445291 BCN116445291 BCN 116445291BCN-116445291-B

Abstract

The invention belongs to the technical field of microbial degradation. The invention provides a maple vein bacterium (Phlebiaacerina) S-LWZ20190614-6CGMCC No.40196 and application thereof in degrading lignin and bisphenol S, the strain is preserved in China general microbiological culture Collection center (CGMCC) for short in 6-month-6 of 2022, the preservation number of the strain is CGMCC No.40196, and the central address is North Star Xili No. 1 and 3 in the Korean area of Beijing city. The degradation rate of the strain S-LWZ20190614-6 provided by the invention reaches 99.6% in 7 days under PDB condition, and reaches 96.2% in 7 days under Kirk condition.

Inventors

  • ZHOU LIWEI
  • LIU SHILIANG
  • WANG JIANQIAO

Assignees

  • 中国科学院微生物研究所
  • 广州大学

Dates

Publication Date
20260505
Application Date
20220812

Claims (8)

  1. 1. The preservation number of the maple vein bacterium (Phlebia acerina) S-LWZ20190614-6 is CGMCC No.40196.
  2. 2. A microbial agent comprising the maple growth pulse bacterium (Phlebia acerina) S-LWZ20190614-6 of claim 1.
  3. 3. A composition capable of degrading lignin and bisphenol S comprising the p.maple (Phlebia acerina) S-LWZ20190614-6 of claim 1.
  4. 4. Use of the maple vein generator (Phlebia acerina) S-LWZ20190614-6 of claim 1 for degrading lignin.
  5. 5. The use of p.maple growth (Phlebia acerina) S-LWZ20190614-6 in the degradation of lignin according to claim 4, wherein the lignin is present in straw or wood.
  6. 6. The use of maple vein-forming bacteria (Phlebia acerina) S-LWZ20190614-6 for degrading lignin according to claim 5, wherein the straw is wheat straw, corn straw or white bamboo straw.
  7. 7. The use of p.maple growth (Phlebia acerina) S-LWZ20190614-6 for degrading lignin according to claim 5, wherein the wood is beech or pine.
  8. 8. The use of the maple vein bacterium (Phlebia acerina) S-LWZ20190614-6 in the degradation of bisphenol S according to claim 1.

Description

Acer maple bioluminescence strain S-LWZ20190614-6 and application thereof in degradation of lignin and bisphenol S Technical Field The invention belongs to the technical field of microbial degradation. Background Lignocellulose is one of the most abundant renewable energy sources in nature, and is also the largest raw material for storing fragrances in nature, the annual output of the lignocellulose reaches 1500 hundred million tons worldwide, and the output of the straw alone reaches 60 hundred million tons. The lignocellulose raw material mainly comprises lignin, cellulose and hemicellulose, wherein the hemicellulose is tightly connected with lignin molecules through covalent bonds and hydrogen bonds, so that the natural lignocellulose has a very firm structure and has strong stress resistance to enzymatic and microbial hydrolysis and other effects. Because of the complex structure of lignin, development of fermentable sugar and high added value products generated by enzymolysis of cellulose and hemicellulose forms a certain obstacle, so that effective removal of lignin also affects effective utilization of cellulose and hemicellulose. The main reason for the difficulty in natural degradation of the straw is that lignocellulose which is a main component of the plant cell wall is difficult to naturally and efficiently degrade, and the straw is an important renewable resource and has important significance for reasonable resource utilization. White rot fungi are main lignin degrading microorganisms, fungi in nature degrade lignin by producing laccase, lignin peroxidase, manganese peroxidase and the like, straw is pretreated by using the white rot fungi, and the lignin structure of the straw is destroyed, so that screening white rot fungi for efficiently degrading lignin is very important for rapidly degrading lignin of the straw. Bisphenol S (BPS) is an important substitute for bisphenol a (BPA) and is commonly used as an additive in daily necessities such as polycarbonate plastics, adhesives, cans, baby bottles, beverage packages, paper currency, cartons, flyers, newspapers, and thermal bill sheets. However, a great deal of research has shown in recent years that bisphenol S has similar toxicity to bisphenol A. In recent years, studies have found 4 new bisphenol S analogues in thermal paper and common paper products, and all of these 4 substances have been demonstrated to have one or more of the above-mentioned toxicities. This suggests that bisphenol S analogues are not completely safe. Bisphenol S detected in water and sediment samples in China has been studied to have a higher concentration. Studies show that bisphenol S is also a compound with potential endocrine effect, the estrogen effect of the bisphenol S is probably not inferior to that of bisphenol A, and bisphenol S molecules are difficult to remove bisphenol S by the traditional water treatment technology because of higher stability, so that bisphenol S is widely detected in various water bodies and soils and even human urine. Because of the many negative effects of bisphenol S, it is difficult to safely and rapidly degrade bisphenol S in the prior art, and therefore, development of an efficient and environmentally friendly method for degrading bisphenol S is needed. Disclosure of Invention In view of this, the present invention provides a strain of Neurospora maple (Phlebia ACERINA PECK) S-LWZ20190614-6 CGMCC No.40196. The invention also provides a microbial agent, which comprises the maple vein bacterium (Phlebia acerina) S-LWZ20190614-6 CGMCC No.40196. The third invention provides a composition comprising the maple vein bacterium (Phlebia acerina) S-LWZ20190614-6 CGMCC No.40196. The invention finally provides application of the maple vein forming bacteria (Phlebia ACERINA PECK) S-LWZ20190614-6 in degrading lignin and bisphenol S. Further, in a specific embodiment of the invention, the lignin is present in straw or wood. Still further, in a specific embodiment of the present invention, the straw is wheat straw, corn straw or white bamboo straw. Still further, in a specific embodiment of the present invention, the wood is beech or pine. The degradation rate of the strain S-LWZ20190614-6 provided by the invention reaches 99.6% in 7 days under PDB condition, and reaches 96.2% in 7 days under Kirk condition. . Drawings FIG. 1 is a graph showing the lignin degradation rate of strain S-LWZ20190614-6 on wheat straw powder, corn straw powder, beech wood powder, white bamboo powder and pine wood powder. FIG. 2 is a graph of degradation of bisphenol S by strain S-LWZ 20190614-6. Detailed Description Example 1 1. Obtaining, isolating and identifying strains The strain S-LWZ20190614-6 is collected in the national natural protection area of the eighth-period of the Shaoguan city, guangdong, 6.14 days, obtained from fresh fruiting bodies grown on forest decay wood by adopting a tissue separation method, and is cultured for 10 days at 28 ℃ after purificatio