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CN-116459274-B - Eucommia ulmoides extract composition, preparation method and application

CN116459274BCN 116459274 BCN116459274 BCN 116459274BCN-116459274-B

Abstract

The invention belongs to the field of medicines, and particularly relates to an eucommia ulmoides extract composition, a preparation method and application. The composition comprises, by weight, 5-10 parts of eucommia polysaccharide, 5-15 parts of rutin, 5-15 parts of hyperin, 1-5 parts of isochlorogenic acid C, 1-5 parts of apigenin and 1-5 parts of oleanolic acid. The preparation method comprises mixing eucommia polysaccharide, rutin, hyperoside, isochlorogenic acid C, apigenin and oleanolic acid or adding solvent and mixing. Has remarkable synergistic effect for treating the membranous nephropathy, thereby providing a new idea for preventing and/or treating diseases related to the membranous nephropathy, the preparation method is simple, is suitable for large-scale production, popularization and use, and reduces the cost.

Inventors

  • Tu Shuicai
  • XIAO QING
  • LUO ZHIYONG
  • LIU LIMING
  • YAN HAIYING
  • LIU SHENG

Assignees

  • 江西普正制药股份有限公司

Dates

Publication Date
20260512
Application Date
20230509

Claims (5)

  1. 1. The eucommia ulmoides extract composition is characterized by comprising, by weight, 5 parts of eucommia ulmoides polysaccharide, 15 parts of rutin, 15 parts of hyperin, 3 parts of isochlorogenic acid C, 1 part of apigenin and 3 parts of oleanolic acid; The preparation method of the eucommia ulmoides polysaccharide comprises the following steps: (1) Adding solvent into cortex Eucommiae, and extracting to obtain extractive solution; (2) Carrying out enzymolysis on the extracting solution to obtain an enzymolysis solution; (3) Precipitating the enzymolysis solution with ethanol; The enzyme adopted by the enzymolysis comprises saccharifying enzyme, beta-glucanase and cellulase; The addition amounts of saccharifying enzyme, beta-glucanase and cellulase adopted by enzymolysis are respectively 0.3%, 0.05% and 0.3% of the mass of the extracting solution, and the alcohol precipitation adopts fractional precipitation, specifically adopts 50% ethanol aqueous solution, 85% ethanol aqueous solution and 85% ethanol aqueous solution containing 8% acetone in sequence.
  2. 2. The eucommia ulmoides extract composition according to claim 1, wherein the solvent is an aqueous solution containing 0.05 to 0.1% by mass of lecithin, and the mass of the aqueous solution is 5 to 10 times that of eucommia ulmoides bark.
  3. 3. A method for preparing the eucommia ulmoides extract composition according to claim 1 or 2, which comprises the step of mixing eucommia ulmoides polysaccharide, rutin, hyperin, isochlorogenic acid C, apigenin and oleanolic acid or adding a solvent and mixing.
  4. 4. The use of the eucommia ulmoides extract composition of claim 1 or 2 or the eucommia ulmoides extract composition prepared by the preparation method of claim 3 in preparing medicines for preventing and/or treating membranous nephropathy.
  5. 5. A pharmaceutical preparation for preventing and/or treating diseases related to membranous nephropathy is characterized by comprising the eucommia ulmoides extract composition of claim 1 or 2 or the eucommia ulmoides extract composition prepared by the preparation method of claim 3, and further comprising a pharmaceutically acceptable carrier, wherein the carrier is any one or more of a slow release agent, a filling agent, an adhesive, a wetting agent, a disintegrating agent, an absorption accelerator, an adsorption carrier, a surfactant or a lubricant, and the drug is any one of an external preparation, an oral preparation or an injection preparation.

Description

Eucommia ulmoides extract composition, preparation method and application Technical Field The invention belongs to the field of medicines, and particularly relates to an eucommia ulmoides extract composition, a preparation method and application. Background Membranous nephropathy (membranous nephropathy, MN), also known as membranous glomerulonephritis (membranous glomerulonephritis), is characterized pathologically by diffuse deposition of immune complexes under glomerular basement membrane epithelial cells with diffuse thickening of the basement membrane. Clinically, nephrotic Syndrome (NS) or asymptomatic proteinuria is the main manifestation. Eucommia ulmoides is usually used as a Chinese medicinal material and is combined with other medicinal materials for treating diseases such as diabetic nephropathy. A Chinese patent application 201810071132.1 discloses a Chinese medicinal composition for preventing and curing diabetic nephropathy and its preparation method. The traditional Chinese medicine composition is mainly prepared from the following raw materials, by weight, 550-650 parts of astragalus membranaceus, 150-250 parts of radix puerariae, 150-250 parts of eucommia ulmoides, 150-250 parts of centella asiatica, 100-200 parts of periostracum cicada, 100-200 parts of silkworm larva, 100-200 parts of burdock, 40-80 parts of rheum officinale and 100-200 parts of turmeric. The invention has precise and appropriate prescription, obvious curative effect, combined use of the medicines, pungent and bitter properties, and simultaneous application of the functions of invigorating kidney, removing blood stasis and opening the Xuanfu. Has better curative effect in reducing urine protein and improving kidney function, can improve general symptoms of diabetic nephropathy, protect kidney function, delay the progress of diabetic nephropathy, and is related to up-regulating the expression of podocyte related protein nephrin, podocin. However, the use of the raw materials as a medicinal composition is very limited. Therefore, the application of eucommia ulmoides extracts and other traditional Chinese medicine extracts in foot cell injury and related diseases of membranous nephropathy is further researched, a new thought is provided for preventing and/or treating related diseases, the defects that side effects are difficult to determine and the substance action basis is complex and pharmacology is undefined due to various components in the conventional traditional Chinese medicine preparation can be further overcome, and the treatment effect is greatly improved. Disclosure of Invention In order to overcome the defects, the invention provides an eucommia ulmoides extract composition, a preparation method and application thereof in doxorubicin-induced human podocyte injury. The eucommia ulmoides extract composition has better effect of preventing and/or treating diseases related to human foot cell injury, so that a new thought is provided for preventing and/or treating diseases related to foot cell injury. In one aspect, the invention provides an eucommia ulmoides extract composition which comprises eucommia ulmoides polysaccharide, rutin, hyperin, isochlorogenic acid C, apigenin and oleanolic acid. Preferably, the eucommia ulmoides polysaccharide comprises, by weight, 5-10 parts of eucommia ulmoides polysaccharide, 5-15 parts of rutin, 5-15 parts of hyperin, 1-5 parts of isochlorogenic acid C, 1-5 parts of apigenin and 1-5 parts of oleanolic acid. Preferably, the eucommia ulmoides polysaccharide comprises, by weight, 5-8 parts of eucommia ulmoides polysaccharide, 10-15 parts of rutin, 10-15 parts of hyperin, 3-5 parts of isochlorogenic acid C, 1-2 parts of apigenin and 3-5 parts of oleanolic acid. Preferably, the eucommia ulmoides polysaccharide comprises, by weight, 5 parts of eucommia ulmoides polysaccharide, 15 parts of rutin, 15 parts of hyperin, 3 parts of isochlorogenic acid C, 1 part of apigenin and 3 parts of oleanolic acid. Preferably, the preparation method of the eucommia ulmoides polysaccharide comprises the following steps: (1) Adding solvent into cortex Eucommiae, and extracting to obtain extractive solution; (2) Carrying out enzymolysis on the extracting solution to obtain an enzymolysis solution; (3) And (3) precipitating the enzymolysis liquid with alcohol. Preferably, the solvent is an aqueous solution containing 0.05-0.1% lecithin, and the mass of the aqueous solution is 5-10 times of that of cortex eucommiae. Preferably, the enzyme adopted by the enzymolysis comprises saccharifying enzyme, beta-glucanase and cellulase, and the addition amounts of the saccharifying enzyme, the beta-glucanase and the cellulase are respectively 0.1-0.5%, 0.05-0.1% and 0.1-0.5% of the mass of the extracting solution; preferably, the alcohol precipitation adopts fractional precipitation, and specifically adopts 45-55% ethanol water solution, 80-90% ethanol water solution and 80-90% ethanol water solution containing 5-10% a