CN-116536052-B - Self-protection type secondary salinization soil restoration microbial inoculum, and preparation method and application thereof

Abstract

The invention relates to a self-protection type secondary salinization soil restoration microbial inoculum, a preparation method and application thereof, wherein the self-protection type secondary salinization soil restoration microbial inoculum comprises the following raw materials, by weight, 5% -15% of bacillus megaterium fermentation liquor, 30% -45% of nitrate-containing industrial wastewater, 30% -40% of organic carbon sources and the balance of water, the mass concentration of nitrate in the nitrate-containing industrial wastewater is greater than 30%, wherein the effective viable count is more than or equal to 2 hundred million/mL, the preparation method comprises the steps of adding the organic carbon sources into the treated nitrate-containing industrial wastewater in proportion for uniform mixing, adding the bacillus megaterium fermentation liquor in proportion, supplementing the bacillus megaterium fermentation liquor with the water to 100%, and standing for at least 12 hours after uniform mixing, and carrying out fertilizer restoration when the self-protection type secondary salinization soil restoration microbial inoculum is applied to secondary salinization soil to be restored after dilution. The soil remediation microbial inoculum disclosed by the invention is liquid, can be stored for up to 3 years in a high-salt environment, and has good self-protection property and salt tolerance.

Inventors

• KAN YUCHEN
• JIN MINGDI
• HUANG LULU
• CHEN LU
• YAN LONGXIANG
• HUO XIAOYU
• ZHOU PEI
• ZHANG DAN
• CHU SHAOHUA
• LI JIANYONG
• DONG YANXIAO

Assignees

• 上海绿乐生物科技有限公司
• 上海交通大学

Dates

Publication Date

20260505

Application Date

20220125

Claims (7)

1. 1. The self-protection type secondary salinization soil remediation microbial inoculum is characterized by comprising the following raw materials, by weight, 5% -15% of bacillus megatherium fermentation liquor, 30% -45% of industrial wastewater containing nitrate, 30% -40% of an organic carbon source and the balance of water, wherein the mass concentration of nitrate in the industrial wastewater containing nitrate is more than 30%; The effective viable count in the self-protection type secondary salinization soil remediation microbial inoculum is more than or equal to 2 hundred million/mL, the total organic matter content is more than or equal to 15wt%, and the total nutrient content is more than or equal to 5wt% according to N+P 2 O 5 +K 2 O; the bacillus megatherium is NCT-2; the organic carbon source is one or the combination of more than one of molasses, soluble starch, glucose, potassium fulvate, potassium humate and yeast waste liquid, and the organic matter content in the organic carbon source is at least 40wt%.
2. 2. The self-protection type secondary salinization soil remediation microbial inoculum according to claim 1, wherein the bacillus megatherium fermentation broth is obtained by fermenting bacillus megatherium, and the effective viable count in the bacillus megatherium fermentation broth is 40-100 hundred million/mL.
3. 3. The self-protecting type secondary salinization soil remediation microbial inoculum according to claim 1, wherein the source of industrial wastewater containing nitrate is one of metal processing industry, electroplating processing industry, food processing industry and fertilizer processing industry.
4. 4. The method for preparing the self-protecting type secondary salinization soil restoration microbial inoculum according to any one of claims 1-3, which is characterized by comprising the following steps: (1) The nitrate-containing industrial wastewater in the food processing industry and the fertilizer industry can be directly used, or the nitrate-containing industrial wastewater produced in the metal processing industry and the electroplating processing industry can be treated by a chelating precipitation method to remove heavy metal ions, until no precipitation is generated, the heavy metal ions can be treated as removed; (2) Adding an organic carbon source into the industrial wastewater containing nitrate treated in the step (1) in proportion for uniform mixing, adding bacillus megatherium fermentation liquor in proportion and supplementing the fermentation liquor with water to 100%, and standing for at least 12 hours after uniform mixing to obtain the self-protection type secondary salinization soil restoration microbial inoculum.
5. 5. The use of the self-protecting type secondary salinized soil restoration microbial agent according to any one of claims 1 to 3, wherein the use method is that the self-protecting type secondary salinized soil restoration microbial agent is firstly subjected to fertilizer restoration and then applied to the secondary salinized soil to be restored.
6. 6. The application of the self-protection type secondary salinization soil restoration microbial inoculum according to claim 5, wherein the self-protection type secondary salinization soil restoration microbial inoculum and water are prepared into a diluent according to a mass ratio of 1 (500-2000), and the diluent is applied after being placed for 1 day.
7. 7. The application of the method for repairing the secondary salinized soil according to claim 6, wherein the diluent is used as a base fertilizer or an additional fertilizer and is subjected to flushing, drip irrigation or infiltrating irrigation in the soil to be repaired according to 500-5000 mL/mu, and the effective viable count of the bacillus megaterium contained in the diluent is more than 2 hundred million/mL.

Description

Self-protection type secondary salinization soil restoration microbial inoculum, and preparation method and application thereof Technical Field The invention relates to the technical field of secondary salinization soil remediation, in particular to a self-protection type secondary salinization soil remediation microbial inoculum, a preparation method and application thereof. Background The facility agriculture causes a plurality of production problems of secondary salinization of soil, unbalanced nutrient, acidification of soil and the like due to special ecological environment and unreasonable water and fertilizer management measures, wherein the most prominent is the secondary salinization of the soil. Soil secondary salinization seriously affects nutrient absorption of crops, leads to physiological drought of the crops and the like, and causes crop yield reduction, and the crop yield reduction leads farmers to have to increase fertilizer input, which aggravates soil secondary salinization, causes vicious circle and seriously affects economic benefits of the farmers. The microbial method has the advantages of low cost, convenient operation, no secondary pollution and the like for repairing the secondary salinized soil, and becomes the first choice method for repairing the secondary salinized soil. The liquid soil restoration microbial inoculum can accurately design an application formula according to actual demands and select a fertilization mode, so that the fertilizer cost is saved, the fertilizer utilization rate is improved, and the liquid soil restoration microbial inoculum is widely used for economic crop planting. With the increasing prominence of the problem of large-area secondary salinization in China, the research of liquid microbiological method for treating nitrate nitrogen has wide prospect, but in the current process of microbial remediation of secondary salinized soil, the main problems are that the quality guarantee period of the liquid soil remediation microbial inoculum is only about 6 months under the condition of adding the protective agent, the quality guarantee period is very short, the infectious microbe is easy to dye in the process of transportation, the storage time is short, the split charging risk is high, the cost of the protective agent is high, most of the protective agents have the functions of inhibiting the growth of the bacterial, the bacterial count in the bacterial liquid is stable, the use efficiency of the bacterial agent is weakened to a certain extent, and secondary pollution is caused by some protective agents, so that the application of the microbial remediation method in the process of remedying the secondary salinized soil is severely limited. The waste water containing high concentration nitrate is widely distributed in metal processing, electroplating, nuclear industry, food processing and other industries, and is characterized by high salt content, small water quantity and strict discharge standard. However, the high-concentration nitrate wastewater is directly discharged, which can cause the phenomena of exceeding of the salt content of the water body, eutrophication, malodor and the like. Therefore, wastewater treatment is required, and in the wastewater treatment, the related nitrate treatment method generally considers a plurality of biological methods including a traditional anoxic-aerobic method, a short-cut nitrification-denitrification method, an anaerobic ammoxidation method and the like, wherein the nitrate is converted into nitrite and further reduced into nitrogen by utilizing the reduction effect of microorganisms. Related derivative denitrification techniques include microbial fuel cells, constructed wetlands, and the like. However, the method can generate greenhouse gases to harm the atmosphere, and on the other hand, the method is influenced by the high-salt characteristic of the wastewater, and partial industrial wastewater lacks enough organic matters as a carbon source to obviously inhibit the reproduction of microorganisms so as to destroy biological denitrification. The waste liquid with high salt concentration containing nitrate has a certain killing effect on most of bacteria intolerant to salt due to the excessively high osmotic pressure, and is unfavorable for the preparation of microbial fertilizer, so the waste water with high concentration of nitrate is not suitable for being treated by adopting a biological method. In the treatment of high-concentration nitrate waste water, a physicochemical method is also a common method, and mainly comprises the following steps of (1) adding metal particles, metal salts or metal oxides into the high-concentration nitrate waste water, rapidly removing the nitrate concentration in the waste water by catalytic reduction under the auxiliary action of light or electricity, wherein more zero-valent nano iron and divalent ferric salt are applied, (2) obtaining clean water from the nitrate-containing