CN-116536332-B - Tomato glandular epidermis coat regulation gene SlWRKY42 and application thereof

CN116536332BCN 116536332 BCN116536332 BCN 116536332BCN-116536332-B

Abstract

The invention relates to the technical field of plant molecular biology, and particularly discloses a tomato glandular epidermal hair regulation gene SlWRKY42 and application thereof, wherein the ORF sequence of the gene SlWRKY42 is shown as SEQ ID NO.1, the density of tomato glandular hair is obviously increased by regulating and controlling the overexpression of the SlWRKY42 gene in a tomato plant, the transcriptional expression of an enal synthase gene (SlTPS) and a synthetic precursor approach transgene is obviously up-regulated, and the content of enal substances is obviously increased. The gene SlWRKY42 provided by the invention can regulate and control the growth and development of the tomato glandular epidermis coat, and simultaneously regulate and control the synthesis of the limonene substance.

Inventors

HU XIAOHUI
LIU XIAOYAN
LI XIAOJING
YANG JIANYU
SHANG CHUNYU
DUAN PENGYU
CHEN GUO
WANG FAN

Assignees

西北农林科技大学

Dates

Publication Date: 20260508
Application Date: 20230510

Claims (3)

1. The application of the SlWRKY42 gene in tomato pest and disease damage defense is characterized in that the excessive expression of the SlWRKY42 gene in tomato plants is regulated, the density of tomato glandular hairs is increased, the excessive expression of the SlWRKY42 gene in tomato plants is regulated, the increase of the content of alkene terpene substances is promoted, the tomato pest and disease damage is bemisia tabaci, and the full-length sequence of the ORF of the SlWRKY42 gene is shown as SEQ ID NO. 1.
2. The application of the overexpression vector of the SlWRKY42 gene in the defense of tomato diseases and insect pests is characterized in that the purpose of the defense of the tomato diseases and insect pests is achieved by promoting the growth and development of the glandular surface coat of the tomato and the synthesis of alkene terpene substances, the tomato diseases and insect pests are bemisia tabaci, the overexpression vector of the SlWRKY42 gene is obtained by connecting the SlWRKY42 gene to a PHELLSGATE framework vector, and the full-length sequence of the ORF of the gene SlWRKY42 is shown as SEQ ID NO. 1.
3. The application of the tomato glandular epidermis hair regulation gene SlWRKY42 or the expression vector of the SlWRKY42 gene in tomato breeding is characterized in that the full-length sequence of the ORF of the gene SlWRKY42 is shown as SEQ ID NO.1, the expression vector of the SlWRKY42 gene is obtained by connecting the SlWRKY42 gene to PHELLSGATE skeleton vectors, and the expression vector of the SlWRKY42 gene or the expression vector of the SlWRKY42 gene is used for promoting the growth and development of tomato epidermis hair and the secretion of terpenoid substances.

Description

Tomato glandular epidermis coat regulation gene SlWRKY42 and application thereof Technical Field The invention relates to the technical field of plant molecular biology, in particular to a tomato glandular epidermis coat regulation gene SlWRKY42 and application thereof. Background Tomato (Solanum lycopersicum L.) is a commercial crop which is favored by consumers because of the nutrients such as lycopene, carotene, multivitamins and the like contained in fruits, has high nutritive value, and is also a model plant important in researching gene functions. At present, in model plant Arabidopsis thaliana, the growth and development of epidermal hair and the regulation mechanism are clear. However, most of the epidermal hairs in Arabidopsis thaliana are single-cell non-secretory epidermal hairs, and there is a large difference between the epidermal hairs and tomato epidermal hairs. There are many types of epidermal hair in tomato, and the regulatory mechanism in multicellular secretory epidermal hair is not yet clear. The non-secreted epidermal hair on the surface of tomato plants is a physical barrier to the activity of phytophagous pests, and the secreted epidermal hair can also secrete chemical substances with an insect-resistant effect. Therefore, research on the development mechanism of the surface coat hair formation of the tomato leaf of an important economic crop and the role played by the surface coat hair in the insect-resistant process have important significance for enhancing the resistance of plants to biotic stress and providing a novel insect-resistant strategy. An enapenoid is known as a defensive substance. Agricultural sustainability development requires that the application of pesticides be replaced by an increase in plant defenses itself during the pest defenses. WRKY transcription factors play an important role in plant response to biotic and abiotic stress, but there is less research in tomato concerning its regulation of epidermal hair growth and terpenoid synthesis. The research of the genes related to regulation and control of the formation of the surface skin hair of the tomato leaf and the synthesis of terpenoid is significant to the application research of tomato diseases and insect pests. Disclosure of Invention In order to obtain a gene for regulating and controlling the formation of surface coat hair of tomato leaves and the synthesis of terpenoid substances, the invention provides a tomato gland type coat hair regulating gene SlWRKY42 and application thereof, and the gene SlWRKY42 provided by the invention can regulate and control the growth and development of the tomato gland type coat hair and simultaneously regulate and control the synthesis of the terpenoid substances. The invention provides a tomato gland type epidermis hair regulating gene SlWRKY42, wherein the full-length sequence of ORF of the gene SlWRKY42 is shown as SEQ ID NO. 1. The invention also provides an overexpression vector of the SlWRKY42 gene, wherein the overexpression vector of the SlWRKY42 gene comprises a target fragment of the full-length sequence of the ORF of the SlWRKY42 gene. Further, the skeleton vector of the overexpression vector of the SlWRKY42 gene is PHELLSGATE vectors. The invention also provides a construction method of the SlWRKY42 gene over-expression vector, which comprises the following steps: S1, using cDNA (complementary deoxyribonucleic acid) formed by reverse transcription of total RNA of tomato tissues as a template, and adopting an amplification primer to amplify an ORF region of a SlWRKY42 gene to obtain a target gene fragment; s2, connecting the target gene fragment to a linearized PHELLSGATE framework vector, and obtaining the overexpression vector of the SlWRKY42 gene after transformation. Further, in S1, the amplification primer comprises an upstream primer FW with a nucleotide sequence shown as SEQ ID NO.2 and a downstream primer RV with a nucleotide sequence shown as SEQ ID NO. 3. The invention also provides application of the gene SlWRKY42 in tomato pest and disease damage defense, and the gene SlWRKY42 is used for regulating and controlling growth and development of tomato glandular epidermis coat and synthesis of enal substances to achieve the purpose of tomato pest and disease damage defense. Further, the density of the tomato glandular hairs is increased by regulating and controlling the overexpression of the SlWRKY42 gene in tomato plants. Further, the increase of the content of the alkenal substances is promoted by regulating and controlling the overexpression of the SlWRKY42 gene in tomato plants. The invention also provides application of the SlWRKY42 gene overexpression vector in tomato pest and disease damage defense, and the purpose of tomato pest and disease damage defense is achieved by regulating and controlling the growth and development of the surface coat of the glands of the tomatoes and the synthesis of the limonene substances. The invention provides applic