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CN-116589104-B - Method for promoting algae bacteria biomembrane to repair arsenic-containing water body by using manganese ions

CN116589104BCN 116589104 BCN116589104 BCN 116589104BCN-116589104-B

Abstract

The invention discloses a method for promoting algae bacteria biomembrane to repair arsenic-containing water body by utilizing manganese ions, and relates to the field of water treatment. The method comprises the following steps of (1) collecting aquatic organism crust as a microorganism source, culturing in a culture medium, centrifuging to obtain an algae bacterial biomembrane, and (2) adding the algae bacterial biomembrane and divalent manganese ions into a water body to be repaired for culturing. The algae bacteria biological film is prepared by culturing biological components widely existing in the crust in the aquatic environment, and the used algae bacteria biological film has high arsenic (As) tolerance and can grow rapidly in As-containing water. Manganese oxide is generated on the surface of the algae bacteria biological film by utilizing microorganism-mediated bivalent manganese ion oxidation, so As to adsorb or coprecipitate As in water, thereby improving the As fixing capability of the algae bacteria biological film. The algae biofilm and manganese oxide can also rapidly oxidize As (III) into As (V) and stably fix the As (V).

Inventors

  • WANG SHIZHONG
  • CHAO YUANQING
  • TANG YETAO
  • QIU RONGLIANG
  • CHEN CHIYU
  • WANG GUOBAO
  • CHEN DAIJIE
  • LI MENGYAO
  • Feng Zekai
  • CHEN XIAOTING
  • JIANG YUANYUAN
  • YU HANG

Assignees

  • 中山大学

Dates

Publication Date
20260512
Application Date
20230530

Claims (7)

  1. 1. The method for promoting the algae bacteria biological membrane to repair the arsenic-containing water body by using the manganese ions is characterized by comprising the following steps of: (1) Collecting aquatic organism crust as a microorganism source, culturing in a culture medium, and centrifuging to obtain an algae bacterial biomembrane; (2) Adding the algae bacterial biomembrane and divalent manganese ions into the water body to be repaired for culture; in the step (1), the aquatic organism crust is derived from an arsenic-polluted tailing wetland area, and in the step (2), the divalent manganese ion source is MnCl 2 , and the adding amount of the algae bacteria biomembrane is 1-5 g/L.
  2. 2. The method for promoting the repair of an arsenic-containing water body by using a manganese ion according to claim 1, wherein in the step (1), the culture condition is that the temperature is 20-30 ℃, and the culture is carried out for 7-14 days under the conditions of illumination of 12 h and light-shielding 12 h every day.
  3. 3. The method for promoting the repair of an arsenic-containing water body by using manganese ions according to claim 1, wherein in the step (2), the culture condition is that the temperature is 20-30 ℃, the illumination is 12 h and the light-shielding is 12 h each day, the pH buffer solution is added to balance the pH of the water body to be neutral, and the culture time is more than 2 days.
  4. 4. A method for promoting the repair of an arsenic-containing water body by using manganese ions according to claim 3, wherein in the step (2), the pH buffer is 0.1 mmol/L HEPES.
  5. 5. The method for promoting the repair of an arsenic-containing water body by using manganese ions according to claim 1, wherein the content of the divalent manganese ions in the step (2) is 1-60 mg/L.
  6. 6. The method for promoting the repair of arsenic-containing water body by using manganese ions according to claim 1, wherein in the step (1), the culture medium comprises 1.5 g/L NaNO 3 、0.04 g/L K 2 HPO 4 ·3H 2 O、0.075 g/L MgSO 4 ·7H 2 O、0.036 g/L CaCl 2 ·2H 2 O、0.006 g/L citric acid, 0.006 g/L ferric ammonium citrate 、0.001 g/L EDTA、0.02 g/L Na 2 CO 3 、2.86 mg/L H 3 BO 3 、1.81 mg/L MnCl 2 ·4H 2 O、0.222 mg/L ZnSO 4 ·7H 2 O、0.390 mg/L Na 2 MoO 4 ·2H 2 O、0.079 mg/L CuSO 4 ·5H 2 O、0.049 mg/L Co(NO3) 2 ·6H 2 O.
  7. 7. The method for promoting the repair of an arsenic-containing water body by using manganese ions according to claim 1, wherein in the step (1), the centrifugation condition is 2500-3500 rpm and the centrifugation condition is 10-20 min.

Description

Method for promoting algae bacteria biomembrane to repair arsenic-containing water body by using manganese ions Technical Field The invention relates to the field of water treatment, in particular to a method for promoting algae bacteria biomembrane to repair an arsenic-containing water body by utilizing manganese ions. Background Arsenic (As) is the 20 th element of the crust content rank, and is ubiquitous in the environment As an carcinogenic element. Geological processes, rock weathering, mining activities, metal smelting, and the use of As-containing agrochemicals, etc. naturally and artificially, result in serious As pollution of groundwater and surface water worldwide. As has strong migration ability in water, is easy to enter a food chain, can cause various diseases such As digestive discomfort, anemia, neuropathic pain and the like after a human body contacts the As, and can induce various cancers such As skin cancer, lung cancer, liver cancer and the like, and the living safety is extremely threatened, so that the treatment of As polluted water is urgent. As exists in the water body mainly in the forms of pentavalent arsenate [ As (V) ] and trivalent arsenite [ As (III) ] with the toxicity and migration of As (III) being higher than those of As (V). As-containing drainage from geological processes and mining activities is one of the major sources of As in the environment, containing significant amounts of As (III) to be remediated. Traditional As repair techniques are divided into physical, chemical and biological repair methods, and the methods prove that the method can effectively remove the As in the water body, but have limitations in practical application. For example, in physical restoration, functional materials such As biochar, zeolite, clay minerals and the like can be used for quickly adsorbing As, but the limitation of the adsorption capacity of the materials leads to poor persistence and needs later maintenance, chemical restoration uses a chemical precipitator to precipitate As, and subsequently produced sludge containing As causes secondary pollution, and biological restoration uses plants such As ciliate desert-grass, vetiver and the like to absorb As, but the problems of slow plant growth, high nutrient requirement and the like limit the exertion of the plants. Therefore, it is of great importance to find an As treatment strategy that is efficient and that can be widely used in aquatic environments. Disclosure of Invention The invention provides a method for promoting an algae biomembrane to repair an arsenic-containing water body by utilizing manganese ions, which utilizes microorganism components in crust to culture and form the algae biomembrane, and microorganisms can mediate the conversion of divalent manganese ions into manganese oxides on the algae biomembrane, so that the arsenic (As) fixing capability of the algae biomembrane is improved, and the As fixing stability is ensured. In order to solve the technical problems, the invention aims to provide a method for promoting an algae bacterial biomembrane to repair an arsenic-containing water body by using manganese ions, which comprises the following steps of: (1) Collecting aquatic organism crust as a microorganism source, culturing in a culture medium, and centrifuging to obtain an algae bacterial biomembrane; (2) Adding the algae bacterial biomembrane and divalent manganese ions into the water body to be repaired for culture. Preferably, in the step (2), the adding amount of the algae bacteria biological film is 1-5g/L. As a preferred scheme, in the step (1), the culture condition is that the temperature is 20-30 ℃, the light is irradiated for 12 hours and the light is prevented from being used for 12 hours every day, and the culture is carried out for 7-14 days. In the step (2), the culture condition is that the temperature is 20-30 ℃, the light is irradiated for 12 hours and the light is prevented from being used for 12 hours every day, the pH buffer solution is added to balance the pH of neutral water body, and the culture time is more than 2 days. Preferably, in step (2), the pH buffer is 0.1mmol/L HEPES. Preferably, in the step (2), the adding amount of the divalent manganese ion is 1-60mg/L. Preferably, in the step (2), the divalent manganese ion source is MnCl 2. Preferably, in step (1), the medium comprises 1.5g/L NaNO3、0.04g/LK2HPO4·3H2O、0.075g/L MgSO4·7H2O、0.036g/L CaCl2·2H2O、0.006g/L citric acid, 0.006g/L ferric ammonium citrate 、0.001g/L EDTA、0.02g/L Na2CO3、2.86mg/L H3BO3、1.81mg/L MnCl2·4H2O、0.222mg/L ZnSO4·7H2O、0.390mg/L Na2MoO4·2H2O、0.079mg/L CuSO4·5H2O、0.049mg/L Co(NO3)2·6H2O. Preferably, in step (1), the aquatic organism crust is derived from an As contaminated tailings wetland area. As a preferred embodiment, the centrifugation conditions are 2500-3500rpm for 10-20min. Compared with the prior art, the embodiment of the invention has the following beneficial effects: 1. The algae bacteria biological film is