Search

CN-116616161-B - Root division method for root zone-root zone communication research and application thereof

CN116616161BCN 116616161 BCN116616161 BCN 116616161BCN-116616161-B

Abstract

The invention relates to the technical field of plant root division, in particular to a root division method for root zone-root zone communication research and application thereof. The root dividing method comprises the steps of (1) seedling cultivation, namely, performing water planting on germinated seeds to obtain seedlings, (2) seedling root cutting, namely, when lateral roots of the seedlings grow to 3-4 cm in step (1), cutting off the main roots to obtain seedlings with the cut main roots, (3) double-stock grafting of the seedlings, namely, selecting two seedlings with the same growth vigor and with the cut main roots to perform double-stock grafting to obtain grafted seedlings, (4) cultivation of the grafted seedlings, namely, completely immersing root systems of the grafted seedlings in a culture solution, culturing until grafting openings are completely healed, and new roots are grown from both the stock A and the stock B to obtain seedlings with the cut roots, and (5) root dividing, namely, transferring the seedlings with the cut roots into a root dividing box to perform root dividing treatment. The root dividing mode can reflect the communication condition of substances among root systems more accurately and intuitively.

Inventors

  • Zhou Lanjuan
  • SUN HONGCHUN
  • BAI ZHIYING
  • TIAN SHIJUN
  • ZHANG XIAOQI
  • ZHANG KE
  • CHEN YIXIN
  • LI CUNDONG
  • LIANG MENGYAO
  • LIU LIANTAO
  • ZHANG YONGJIANG

Assignees

  • 河北农业大学

Dates

Publication Date
20260505
Application Date
20230705

Claims (6)

  1. 1. A root splitting method for root zone-root zone communication research, comprising the steps of: (1) Seedling cultivation, namely performing water planting on germinated seeds to obtain seedlings; (2) Cutting off the main root of the seedling when the lateral root of the seedling in the step (1) grows to 3-4 cm, so as to obtain the seedling with the main root; (3) Double-stock grafting of seedlings, namely selecting two seedlings with the same growth vigor and with main roots for double-stock grafting to obtain grafted seedlings; (4) Culturing the grafted seedlings, namely, completely immersing the root systems of the grafted seedlings in a culture solution, and culturing the grafted seedlings with the grafting openings outside the culture solution until the grafting openings are completely healed and new roots grow out from both the stock A and the stock B, so as to obtain seedlings to be divided into roots; (5) Root division, namely moving the seedlings to be root-divided into root division boxes for root division treatment; the seedlings with the cut main roots retain 1-2 cm of main root bases; The double-stock grafting method comprises the following steps of cutting a wedge-shaped incision of 55-70 degrees at a position 1-2 cm below a cotyledon node of a seedling of a main root to obtain a stock A, cutting only one cotyledon at the top of the seedling of the main root, cutting a wedge-shaped incision of 55-70 degrees at a position 1-2 cm below the cotyledon node of the seedling of the main root to obtain a stock B, parallelly jointing the wedge-shaped incisions of the stock A and the stock B, and fixing a grafting port to finish grafting; The root dividing treatment method in the step (5) comprises the steps of dividing the root system of a stock A into two root areas a 1 and a 2 , dividing the root system of a stock B into two root areas B 1 and B 2 , dividing the root areas a 1 、a 2 and B 1 、b 2 into two groups for root dividing; The root areas a 1 and a 2 represent that the newly grown lateral root of the stock A after grafting is equally divided into two root areas; The root regions B 1 and B 2 represent that the newly grown lateral root of the stock B after grafting is equally divided into two root regions; (a 1 +a 2 )/(b 1 +b 2 ) is an independent root grouping; (a 1 +b 1 )/(a 2 +b 2 ) is an interconnection root packet; independent root grouping means that root systems of the stock A and the stock B are connected at the joint of the hypocotyls and are not interconnected by main roots; the interconnection root grouping means that part of root systems of the stock A and the stock B are interconnected by main roots; the seedling is cotton seedling.
  2. 2. The rooting method according to claim 1, wherein the temperature of the hydroponic culture in the step (1) is 24-26 ℃; the illumination intensity of the water culture is 7500-8500 Lx; the humidity of the water culture is 60-70%; The illumination time of the water planting is 15-17 h/d.
  3. 3. The rooting method according to claim 1, wherein the solvent of the culture solution in the step (4) is water; the culture solution comprises the following components in concentration: H 3 BO 3 0.2~0.3 mg/L,MnSO 4 ·H 2 O 0.1~0.2 mg/L,ZnSO 4 ·7H 2 O 0.01~0.05 mg/L,CuSO 4 ·5H 2 O 0.005~0.01 mg/L,（NH 4 ） 6 Mo 7 O 24 ·4H 2 O 0.001~0.003 mg/L, KNO 3 50~52 mg/L,MgSO 4 ·7H 2 O 48~50 mg/L,KH 2 PO 4 10~15 mg/L, NH 4 H 2 PO 4 50~52 mg/L,EDTA-Na 2 3~5 mg/L,FeSO 4 ·7H 2 O 2.5~3 mg/L,Ca（NO 3 ） 2 ·4H 2 O 115~120 mg/L.
  4. 4. the rooting method according to claim 1, wherein the temperature of the culture in the step (4) is 24-26 ℃; The illumination intensity of the culture is 7500-8500 Lx, and the humidity of the culture is 60-70%.
  5. 5. The use of the rooting method according to any one of claims 1-4 in research of non-uniform environments of plants.
  6. 6. Use of the rooting method according to any one of claims 1 to 4 for studying plant root zone-root zone communication.

Description

Root division method for root zone-root zone communication research and application thereof Technical Field The invention relates to the technical field of plant root division, in particular to a root division method for root zone-root zone communication research and application thereof. Background The soil on which the plants depend to survive has heterogeneity, and different root areas in the root system of the same plant can be stimulated by different environments, because the different root areas are mutually connected through the main roots or the root bases which are mutually connected, the natural connection taking the main roots or the root bases as the tie in the root areas can be a structural basis for carrying out signal communication and cooperative response stimulation. The signal communication between roots, mediated by the main root or root base, independent of the earth's upper part is called root zone-root zone communication. Plant rooting is widely used for research on plant response to heterogeneous environments. The root splitting system suitable for researching root zone-root zone communication should realize two root splitting effects, namely ① keeps the root base to naturally connect two groups of root systems, ② is mutually independent and does not need two groups of root systems connected with the root base, and the two root splitting effects can be freely converted on the premise of not changing other variables. The existing common root dividing method can obtain two root areas which keep natural connection or are mutually independent, but cannot realize two root dividing effects on the premise of not changing other variables. Therefore, a scientific root division system is established, which is a precondition for researching root zone-root zone communication. Disclosure of Invention The invention aims to provide a root dividing method capable of realizing the preservation of natural connection and mutual independence between root regions and free conversion without changing other variables, in particular to a root dividing method for root region-root region communication research and application thereof. In order to achieve the above object, the present invention provides the following technical solutions: the invention provides a root dividing method for root zone-root zone communication research, which comprises the following steps: (1) Seedling cultivation, namely performing water planting on germinated seeds to obtain seedlings; (2) Cutting off the main root of the seedling when the lateral root of the seedling in the step (1) grows to 3-4 cm, so as to obtain the seedling with the main root; (3) Double-stock grafting of seedlings, namely selecting two seedlings with the same growth vigor and with main roots for double-stock grafting to obtain grafted seedlings; (4) Culturing the grafted seedlings, namely, completely immersing the root systems of the grafted seedlings in a culture solution, and culturing the grafted seedlings with the grafting openings outside the culture solution until the grafting openings are completely healed and new roots grow out from both the stock A and the stock B, so as to obtain seedlings to be divided into roots; (5) And root division, namely moving the seedlings to be root-divided into root division boxes for root division treatment. Preferably, the temperature of the water culture in the step (1) is 24-26 ℃; the illumination intensity of the water culture is 7500-8500 Lx; the humidity of the water culture is 60-70%; The illumination time of the water planting is 15-17 h/d. Preferably, the seedlings with the cut main roots retain 1-2 cm of main root bases. The double-stock grafting method comprises the following steps of cutting a wedge-shaped incision of 55-70 degrees at a position 1-2 cm below a cotyledon node of a seedling of one trunk, obtaining a stock A, cutting a wedge-shaped incision of 55-70 degrees at the position 1-2 cm below the cotyledon node of the seedling of the other trunk, leaving only one cotyledon at the top of the seedling of the other trunk, cutting a wedge-shaped incision of 55-70 degrees at the position 1-2 cm below the cotyledon node, obtaining a stock B, parallelly jointing the wedge-shaped incisions of the stock A and the stock B, and fixing a grafting port to finish grafting. Preferably, the solvent of the culture solution in the step (4) is water; the culture solution comprises the following components in concentration: H3BO30.2～0.3mg/L,MnSO4·H2O0.1～0.2mg/L,ZnSO4·7H2O0.01～0.05mg/L,CuSO4·5H2O0.005～0.01mg/L,(NH4)6Mo7O24·4H2O0.001～0.003mg/L,KNO350～52mg/L,MgSO4·7H2O48～50mg/L,KH2PO410～15mg/L,NH4H2PO450～52mg/L,EDTA-Na23～5mg/L,FeSO4·7H2O2.5～3mg/L,Ca(NO3)2·4H2O115～120mg/L. Preferably, the temperature of the culture in the step (4) is 24-26 ℃; The illumination intensity of the culture is 7500-8500 Lx, and the humidity of the culture is 60-70%. Preferably, the root dividing treatment method in the step (5) comprises dividing the