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CN-116678852-B - Optical imaging system and imaging method

CN116678852BCN 116678852 BCN116678852 BCN 116678852BCN-116678852-B

Abstract

The invention belongs to the technical field of optics and an imaging method, and discloses an optical imaging system which comprises a light source generating assembly, a light splitting assembly, a sample table and an imaging unit, wherein the light splitting assembly is used for receiving a main light ray emitted by the light source generating assembly and splitting the main light ray to form a first light path and a second light path, and a polarization beam splitting prism is arranged at the junction of the first light path and the second light path. The optical imaging system can enable the reflected light of the object to be detected and the reference light formed by the second light to generate an interference pattern through arranging the polarization beam splitting prism on the beam splitting path, has a simple integral structure, does not need to use complex components, is beneficial to reducing cost and is suitable for industrialization requirements.

Inventors

  • SUN RUI
  • LI FENGHUA
  • NIE YUJIE
  • WANG YI

Assignees

  • 深圳市倍捷锐生物医学科技有限公司

Dates

Publication Date
20260505
Application Date
20230616

Claims (9)

  1. 1. An optical imaging system is characterized by comprising a light source generating assembly, a beam splitting assembly, a sample stage and an imaging unit; the light splitting assembly is used for receiving the main light rays emitted by the light source generating assembly and splitting the main light rays into a first light path and a second light path, and a polarization splitting prism is arranged at the junction of the first light path and the second light path; The light of the first light path irradiates the sample stage to form reflected light and irradiates the polarizing beam splitter prism along the direction reverse to the first light path; The polarization beam splitting prism is used for combining the light rays of the second light path and the reflected light rays to an imaging area of the imaging unit so as to form an interference image; The light splitting assembly is provided with two light outlets respectively corresponding to the first light path and the second light path, and the lens assemblies are arranged between the light outlets and the polarization splitting prism; The lens assembly comprises a second convex lens, a first polarizer and a half-wave plate which are sequentially arranged, wherein the first light path or the light of the second light path passes through the second convex lens to form collimated light, the collimated light passes through the first polarizer to be linearly polarized to form polarized light, and the polarized light passes through the half-wave plate to rotate the polarized light to a preset angle.
  2. 2. The optical imaging system of claim 1, further comprising a first convex lens disposed between the light source generating assembly and the light splitting assembly for focusing chief rays emitted by the light source generating assembly.
  3. 3. The optical imaging system of claim 2, wherein the light source generating assembly is a laser generator and the chief ray is a laser beam.
  4. 4. The optical imaging system according to claim 1, wherein the predetermined position of the first optical path is provided with at least one set of mirrors for changing the irradiation direction of the first optical path.
  5. 5. The optical imaging system according to claim 4, wherein a light splitting lens is disposed on the first optical path opposite to the reflector, a light receiving surface of the light splitting lens is correspondingly disposed with a third optical path, and a white light source component is disposed on the third optical path; The light splitting lens is used for connecting the white light emitted by the white light source assembly to the first light path and irradiating the white light to the sample stage.
  6. 6. The optical imaging system of claim 5, wherein an objective lens is further disposed above the sample stage, the first optical path is disposed through the objective lens, and a tube lens is disposed between the mirror and the beam splitter lens.
  7. 7. The optical imaging system of claim 1, wherein a quarter wave plate is disposed between the polarizing beam splitter prism and the imaging unit.
  8. 8. An imaging method, characterized by being applied to the optical imaging system according to any one of claims 1 to 7, comprising in particular: The light source generating assembly operates to emit a main light ray to the light splitting assembly, and the light splitting assembly splits the main light ray to form a first light ray and a second light ray; the first light irradiates the sample stage along the first light through the polarization splitting prism, and a workpiece of the sample stage reflects the light to form reflected light; the polarization beam splitting prism is used for combining the light rays of the second light path and the reflected light rays to an imaging area of the imaging unit, and the imaging unit performs imaging.
  9. 9. The imaging method of claim 8, wherein the first optical path is further provided with a beam splitting lens, further comprising: the white light source assembly is arranged on a light receiving surface of the light splitting lens, white light emitted by the white light source assembly is connected to the first light path through the light splitting lens, and the white light is used for supplying bright field light source illumination to a workpiece on the sample table.

Description

Optical imaging system and imaging method Technical Field The present invention relates to the field of optical technologies, and in particular, to an optical imaging system and an imaging method. Background Quantitative phase microscopy (QuantitativePhase Microscope, QPM) has become an important tool for material metering and biomedical imaging due to its non-contact, in situ and label-free nature, for example to quantify morphology and dynamics within cells and materials, cancer diagnosis, and analysis and examination of material structure. In order to achieve the effect of quantitative phase imaging, the quantitative phase microscope in the prior art needs to be provided with a digital micromirror device, has a complex light path structure and high cost, and is difficult to become a standard imaging instrument in research laboratories and industries. In view of this, an imaging microscope in the prior art needs to be improved to solve the technical problems of complex optical path structure and high cost of quantitative phase. Disclosure of Invention The invention aims to provide an optical imaging system and an imaging method, which solve the technical problems. To achieve the purpose, the invention adopts the following technical scheme: An optical imaging system comprises a light source generating assembly, a light splitting assembly, a sample stage and an imaging unit; the light splitting assembly is used for receiving the main light rays emitted by the light source generating assembly and splitting the main light rays into a first light path and a second light path, and a polarization splitting prism is arranged at the junction of the first light path and the second light path; The light of the first light path irradiates the sample stage to form reflected light and irradiates the polarizing beam splitter prism along the direction reverse to the first light path; The polarization beam splitter prism is used for combining the light rays of the second light path and the reflected light rays to an imaging area of the imaging unit so as to form an interference image. Optionally, the optical imaging system further includes a first convex lens, where the first convex lens is disposed between the light source generating assembly and the light splitting assembly, and is configured to focus the chief ray emitted by the light source generating assembly. Optionally, the light source generating component is a laser generator, and the main light is a laser beam. Optionally, the optical imaging system further includes two groups of lens assemblies, the light splitting assembly has two light outlets corresponding to the first light path and the second light path respectively, and the lens assemblies are disposed between the light outlets and the polarization splitting prism; The lens assembly comprises a second convex lens, a first polarizer and a half-wave plate which are sequentially arranged, wherein the first light path or the light of the second light path passes through the second convex lens to form collimated light, the collimated light passes through the first polarizer to be linearly polarized to form polarized light, and the polarized light passes through the half-wave plate to rotate the polarized light to a preset angle. Optionally, at least one group of reflectors is disposed at a preset position of the first optical path, and the reflectors are used for changing the irradiation direction of the first optical path. Optionally, a light splitting lens is arranged on the first light path opposite to the reflector, a light receiving surface of the light splitting lens is correspondingly provided with a third light path, and a white light source component is arranged on the third light path; The light splitting lens is used for connecting the white light emitted by the white light source assembly to the first light path and irradiating the white light to the sample stage. Optionally, an objective lens is further disposed above the sample stage, the first optical path passes through the objective lens, and a tube lens is disposed between the reflective mirror and the beam splitting lens. Optionally, a quarter wave plate is arranged between the polarization beam splitter prism and the imaging unit. The invention provides an imaging method, an optical imaging system as described above, specifically comprising: The light source generating assembly operates to emit a main light ray to the light splitting assembly, and the light splitting assembly splits the main light ray to form a first light ray and a second light ray; the first light irradiates the sample stage along the first light through the polarization splitting prism, and a workpiece of the sample stage reflects the light to form reflected light; the polarization beam splitting prism is used for combining the light rays of the second light path and the reflected light rays to an imaging area of the imaging unit, and the imaging unit performs imaging. O