CN-116694755-B - SNP molecular marker related to NOBOX gene pig sex dysplasia, primer and application

Abstract

The invention relates to the technical field of pig selective breeding, in particular to a NOBOX gene and an intermittent pig related SNP molecular marker, a primer and application. The nucleotide sequence of the SNP molecular marker is SEQ ID NO. 1, the 231 st base S in the nucleotide sequence of the SNP molecular marker is a polymorphic site, and the individual sex of the polymorphic site with genotype CC and CG is abnormal in development, and the individual sex with genotype GG is normal in development. The primer of the present invention includes F1 and a downstream primer R1. The SNP molecular marker is closely related to the characteristics of the pig intervenereal disease, can be effectively used for breeding the breeding pigs, prevents the sex dysplasia of the pigs, achieves the prenatal and postnatal care of the pigs, and also provides a reference for the sex dysplasia of human beings.

Inventors

• LI HUA
• Zhong Bingzhou
• ZHAO HAIQUAN
• FENG ZHENG
• WU JINHUA
• YU CONGYING

Assignees

• 佛山科学技术学院

Dates

Publication Date

20260505

Application Date

20230625

Claims (2)

1. 1. The application of a primer of SNP molecular markers related to NOBOX gene pig sex dysplasia in breeding pigs for screening interstitial pigs is characterized in that the nucleotide sequence of the SNP molecular markers is SEQ ID NO. 1, the 231 th base S in the nucleotide sequence of the SNP molecular markers is a polymorphic site, the sex of individuals with genotype CC and CG is dysplasia at the polymorphic site, and the sex of individuals with genotype GG is normal; The SNP molecular marker is positioned on the 5 th exon of NOBOX gene of chromosome 9 of the pig; The SNP molecular marker is used for screening individuals with the genotype GG of the SNP molecular marker as breeding parents in breeding of breeding pigs.
2. 2. The application of the primer of SNP molecular markers related to NOBOX gene pig sex dysplasia in breeding pigs for screening interstitial pigs, as claimed in claim 1, wherein the primer is used for amplifying the SNP molecular markers related to NOBOX gene and interstitial pigs, and comprises an upstream primer and a downstream primer: The upstream primer F1: 5'-TGCTCCACAGACCCAGCTT-3', The downstream primer R1: 5'-GACAATGGAACGCAAGGAACG-3'.

Description

SNP molecular marker related to NOBOX gene pig sex dysplasia, primer and application Technical Field The invention relates to the technical field of pig selective breeding, in particular to SNP molecular markers related to NOBOX gene pig sex dysplasia, primers and application. Background Sex dysplasia (Disorders ofsex development, DSD) refers to a disease in which organisms exhibit abnormalities in sex determination or sex differentiation links during sex development, resulting in inconsistent expression of the chromosome karyotype, gonadal phenotype, and external genitalia. Pigs are mammals, and the sex determination mode is the same as that of human, and the pigs belong to chromosome male heteroleptic type, namely the chromosome karyotype of normal sow is 38, XX, and the chromosome karyotype of boar is 38, XY. It is believed that sex-determining genes (Sex Determining Region Y, SRY) on the Y chromosome allow the gonads of organisms to develop and grow in the direction of males. 38, XX-DSD (Y-) means that a pig of chromosome karyotype (38, XX) has the characteristics of both male and female in the absence of the Y chromosome. Because of the sexual reproduction organs of the male and female, the disease is also called as a pig intervenereal disease, which can cause the reproductive capacity of pigs to be damaged, the urogenital system to be infected, the growth performance to be reduced, and the economic benefit of pig breeding to be seriously reduced. The literature reports that the occurrence frequency of 38, XX-DSD pigs in different groups is 0.08-0.75%, and the occurrence frequency of 38, XX-DSD pigs in some independent inbred groups can reach 20%, but the actual occurrence frequency of 38, XX-DSD pigs is possibly underestimated because of the condition that the 38, XX-DSD pigs have double cryptorches, which is difficult to identify in appearance at a glance. The detection of the genetic defect pathogenic genes of pigs has important biological significance and economic value. Pigs share many similarities with humans, and pig biomedical models that mimic human reproduction greatly promote understanding of the underlying science of disease. Studies of porcine DSD may provide a reference for human DSD. At the end of 2022, china had 45256 thousands of live pigs in stock, and sows in stock 4390 thousands of sows could be bred. Assuming that the annual productivity (productivityper sowperyear, PSY) of the sow is 20 and that the incidence of DSD of the pig is 0.08%, about 70.24 thousands of piglets in China have DSD in one year. If the selling price of one weaned pig is 500 yuan, the pig raising enterprises nationally annually lose 3.512 hundred million yuan. The pathogenic genes of the genetic defect diseases can not be precisely removed from the aspects of improving economic benefit and making breeding strategies. 38, XX-DSD pigs are autosomal polygenic hereditary diseases with a genetic power of up to 0.72-0.81. Most scholars consider that the disease is autosomal recessive inheritance, when at least one of parents is a carrier, the offspring is likely to also carry a pathogenic gene, and the pathogenic gene can be spread through generation inheritance because heterozygotes do not show symptoms. When parents are both carriers, their offspring may be homozygous for the pathogenic gene, resulting in porcine DSD. Particularly, when the boars are carriers, the harm degree is larger, because the artificial insemination technology is the main breeding technology of commercial pig farms in China, and the boar semen of the last boar can be matched with a plurality of sows according to the ratio of 1:80-200. When foreign lean-type breeding pigs are introduced in large scale in China for breeding, it is very important to timely detect individuals carrying pathogenic genes. Therefore, the development and application of SNP molecular markers with 38, XX-DSD specificity to assist in the detection of carriers of pathogenic genes is urgent. In order to optimize reproductive health of humans, prenatal diagnosis has been carried out in recent years, and SNP site patents capable of detecting sex dysplasia have been developed gradually and applied to practice. However, the SNPs molecular markers of the DSD are only applied to human beings and goats, but not applied to animals such as pigs, dogs, horses, cows and the like with the DSD, so that the development of 38, XX-DSD pig SNP molecular markers and application can fill the gap of the international and domestic auxiliary detection tools in the field. Disclosure of Invention The invention aims to provide an SNP molecular marker related to NOBOX gene pig sex dysplasia, a primer and application, wherein the SNP molecular marker is closely related to pig intervenereal disease characters, can be used for genetic diagnosis of breeding pigs and breeding selection based on molecular markers, and can prevent transmission of pig intervenereal disease. To achieve the purpose, the inve