CN-116698929-B - Portable chromatography detection device and application thereof

Abstract

The invention discloses a portable chromatography detection device and application thereof, and belongs to the technical field of detection devices. The device comprises a top cover, a base, a test strip and a flexible PCB circuit board, wherein the test strip comprises a sample pad, a bonding pad, a nitrocellulose membrane, a water absorption pad and a supporting backboard, a patterned electrode is arranged on the nitrocellulose membrane and used as an electric channel, the nitrocellulose membrane is divided into a clamping area and a detection area for solution circulation, the clamping area is connected with the flexible PCB circuit board through a magnetic suction connector, the top of one end of the detection area is in overlapped fit connection with the water absorption pad, the top of the other end of the detection area is in overlapped fit connection with the bonding pad, the top of the other end of the bonding pad is in overlapped fit connection with the sample pad, and hollowed-out windows are respectively formed in the sample pad and the nitrocellulose membrane on the top cover. The detection device provided by the invention is convenient to carry, has multiple functions and high sensitivity and integration level, can be used in combination with equipment such as a computer and a mobile phone, and is particularly suitable for being used in places such as families and fields.

Inventors

• SU BIN
• YING XUDONG
• ZHOU LIN

Assignees

• 浙江大学

Dates

Publication Date

20260512

Application Date

20230606

Claims (8)

1. 1. The portable chromatography detection device is characterized by comprising a top cover (1), a base (9), a test paper strip and a flexible PCB (printed circuit board) (7), wherein the top cover (1) and the base (9) can jointly form a shell structure with a hollow inner cavity, and the test paper strip is detachably arranged in the hollow inner cavity; The test strip comprises a sample pad (2), a bonding pad (3), a nitrocellulose membrane (4), a water absorbing pad (8) and a support back plate (5), wherein the sample pad (2), the bonding pad (3), the nitrocellulose membrane (4) and the water absorbing pad (8) are bonded and fixed with a base (9) through the support back plate (5), the nitrocellulose membrane (4) is provided with a patterned electrode serving as an electric channel and divided into a clamping area and a detection area for solution circulation, the clamping area is connected with a flexible PCB (7) through a magnetic connector (6), one end top of the detection area is in overlapped and bonded connection with the water absorbing pad (8), the other end top of the detection area is in overlapped and bonded connection with the bonding pad (3), the other end top of the bonding pad (3) is in overlapped and bonded connection with the sample pad (2), and hollow windows are respectively formed in the sample pad (2) and the nitrocellulose membrane (4) on a top cover (1); The nitrocellulose membrane (4) is divided into a clamping area and a detection area by a hydrophobic material, wherein the clamping areas are positioned at two sides, and the detection area is positioned in the middle; The patterned electrode comprises one or more working electrodes, a group of counter electrodes and a reference electrode, and the working electrode pattern at least covers the detection line of the test strip.
2. 2. A portable chromatography detection apparatus according to claim 1, wherein a battery (10) for powering the flexible PCB circuit board (7) is further fixed to the base (9).
3. 3. The portable chromatography detection apparatus according to claim 1, wherein the inside of the base (9) is provided with notches for fixing test strips, a magnetic connector (6) and a flexible PCB (7).
4. 4. The portable chromatography detection apparatus according to claim 1, wherein the working electrode pattern further covers a control line of the test strip.
5. 5. The portable chromatography detection apparatus according to claim 1, wherein the electrical analysis method that can be satisfied by the flexible PCB circuit board (7) is cyclic voltammetry, chronoamperometry, differential pulse voltammetry, amperometry, coulometry, linear sweep voltammetry.
6. 6. The portable chromatography detection apparatus according to claim 1, wherein the sample pad (2) is made of glass fiber or polyester fiber, the bonding pad (3) is made of glass fiber deposited with a target detection signal substance, and the water absorbing pad (8) is made of polyester fiber.
7. 7. The portable chromatographic test device according to claim 1, wherein the test strip can be used for detection by electrical signal detection, electrochromic and electrochemiluminescence.
8. 8. Use of a portable chromatography detection apparatus according to any one of claims 1 to 7 in the fields of immunoassay, environmental detection, food detection and health monitoring.

Description

Portable chromatography detection device and application thereof Technical Field The invention belongs to the technical field of detection devices, and particularly relates to a chromatography detection device which is convenient to carry, various in function and high in sensitivity and integration level. Background In recent years, with the enhancement of people's health consciousness, the traditional detection based on large-scale instruments is difficult to meet the requirements of mass and rapid detection of society, and the current situation of uneven distribution of medical resources in China is not met. Based on this, a miniaturized, low-cost, fast detection mode, point-of-CARE TESTING, is becoming more and more favored. The colloidal gold-based test strip is the most commonly used instant detection platform, but at present, qualitative and semi-quantitative detection is still mainly used, and accurate quantification and low sensitivity cannot be realized. The electrochemical detection has the advantages of simplicity, easiness, high sensitivity, good real-time performance and the like, and can be used as a quantitative detection method to be combined with a test strip so as to accurately quantify the target analyte. The current electrochemical test strips are all combined by independent electrodes and test strips. For example, chinese patent CN 204731241U shows an electrochemical blood test strip in which electrodes are printed on an insulating substrate, and then the substrate is placed under a membrane for detecting electrochemical signals, and in addition, wires, hydrophilic membranes, etc. are stacked. The assembly mode needs to consider the fitting degree of the electrode and the test strip, so that the signal can be detected, and in addition, the stacking mode improves the complexity and the cost of the device, and the repeatability of the device is affected. Similar to the Chinese patent such as CN 1908645A, CN102565153A, CN 103175871B, CN 111781351A, the defects exist. The miniaturized and low-cost detection mode depends on a portable detection means. Chaiyo et al realized smart phone based electrochemical test strip detection by inserting electrodes into a USB flash disk type portable electrochemical analyzer and then relying on the cell phone to power and receive the processing signals for on-site and immediate detection (Anal. Chem.2022,94, 2554-2560). However, this method causes destruction of the paper-based electrode, which affects signal reading, and thus development of a new connection method is desired. The new connection mode ensures that the electrochemical analysis is stably carried out and the electrochemical signal is stably read while fixing the test strip. Disclosure of Invention The invention aims to overcome the defects in the prior art and provides a portable chromatography detection device and application thereof. The detection device provided by the invention is convenient to carry, has various functions and high sensitivity and integration level, can be used in combination with equipment such as a computer and a mobile phone, and is particularly suitable for being used in places such as families and fields. The specific technical scheme adopted by the invention is as follows: the invention provides a portable chromatography detection device, which comprises a top cover, a base, a test strip and a flexible PCB (printed circuit board), wherein the top cover and the base can jointly form a shell structure with a hollow inner cavity, and the test strip is detachably positioned in the hollow inner cavity; the test strip comprises a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a support back plate, wherein the sample pad, the combination pad, the nitrocellulose membrane and the water absorption pad are all adhered and fixed with a base through the support back plate, a patterned electrode is arranged on the nitrocellulose membrane and is used as an electric channel and divided into a clamping area and a detection area for solution circulation, the clamping area is connected with a flexible PCB (printed circuit board) through a magnetic connector, the top of one end of the detection area is overlapped and adhered with the water absorption pad, the top of the other end of the detection area is overlapped and adhered with the combination pad, the top of the other end of the combination pad is overlapped and adhered with the sample pad, and hollowed-out windows are respectively formed in the positions of the sample pad and the nitrocellulose membrane on the top cover. Preferably, a battery for supplying power to the flexible PCB is also fixed on the base. Preferably, the nitrocellulose membrane is divided into a clamping area and a detection area by a hydrophobic material, the clamping areas are positioned at two sides, the detection area is positioned in the middle, and the clamping areas at two sides are connected with each other through