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CN-116731869-B - Aspergillus oryzae with multiple tolerability and separation method and application thereof

CN116731869BCN 116731869 BCN116731869 BCN 116731869BCN-116731869-B

Abstract

The invention discloses an aspergillus oryzae with multiple tolerance, a separation method and application thereof, wherein the aspergillus oryzae is a strain A. Oryzae TH004 and is preserved in China general microbiological culture collection center (CGMCC) No.40448. The Aspergillus oryzae is obtained from Daqu by separating, purifying and screening, and has Gao Tanghua force, high temperature resistance, acid resistance, ethanol resistance, etc. The invention can be applied to the production of wines, in particular to distiller's yeast, and can improve saccharification force. The invention has important value in the aspects of saving grains, improving efficiency, saving energy, reducing emission and the like.

Inventors

  • HUANG XUNDUAN
  • GAO QIANG
  • ZHANG BUCHANG
  • WANG YANSHENG
  • Jia Xiulian
  • Li Dengzhuang
  • WANG ZHIXIA
  • ZHOU MING

Assignees

  • 安徽大学

Dates

Publication Date
20260512
Application Date
20230113

Claims (8)

  1. 1. Aspergillus oryzae (Aspergillus oryzae) with multiple tolerance is strain A. Oryzae TH004, and is preserved in China general microbiological culture collection center (CGMCC) No. 40448 at 12-25 of 2022.
  2. 2. An aspergillus oryzae having multiple tolerance according to claim 1, wherein the strain a. Oryzae TH004 grows well at 25-45 ℃.
  3. 3. An aspergillus oryzae having multiple tolerance according to claim 1, wherein the strain a. Oryzae TH004 grows well at a pH of 2-7.
  4. 4. An aspergillus oryzae having multiple tolerance according to claim 1, wherein the strain a. Oryzae TH004 grows well at ethanol levels of 2-6% vol.
  5. 5. An aspergillus oryzae having multiple tolerability according to claim 1, wherein strain a. Oryzae TH004 is optimized for improved gluten saccharification by a response surface method.
  6. 6. The aspergillus oryzae having multiple tolerability of claim 5, wherein the strain a. Oryzae TH004 is significantly improved in saccharification power of the bran inoculated thereto at a temperature of 28 ℃ and a moisture content of 49.54% and an inoculum size of 6.62% as determined by optimization of the response surface method.
  7. 7. Use of an aspergillus oryzae having multiple tolerance according to any one of claims 1-6 in the production of alcoholic beverages.
  8. 8. A method for improving the saccharification power of distiller's yeast, characterized in that the aspergillus oryzae having multiple tolerance according to any one of claims 1-6 is added into distiller's yeast raw material to improve the saccharification power of distiller's yeast.

Description

Aspergillus oryzae with multiple tolerability and separation method and application thereof Technical Field The invention relates to the field of aspergillus oryzae, in particular to aspergillus oryzae with multiple tolerability and a separation method and application thereof. Background A large amount of grains are consumed by white spirit enterprises each year for brewing white spirit, so that the production efficiency is improved, the grain and energy consumption is reduced, and the white spirit brewing method has important values for enterprises and society. The distiller's yeast can provide important microorganisms for brewing white spirit, and the saccharification force determines the utilization rate of grains and the yield of white spirit. Gao Tanghua Streptomyces are developed and used for making distiller's yeast, so that the saccharification force of distiller's yeast can be improved, grain is saved, energy consumption is reduced, and enterprise and social benefits are increased. The process of distiller's yeast preparation and cellar entry fermentation often has more severe environmental conditions, such as high temperature, low pH, high alcohol content, etc. These environmental conditions can seriously affect the growth and survival of the mold, thereby affecting the effectiveness of the mold application. The mould is difficult to simultaneously have the characteristics of Gao Tanghua force resistance, high temperature resistance, acid resistance, ethanol resistance and the like. Therefore, the development of an excellent mold that can satisfy the above conditions at the same time is still an important research direction. In addition, the application effect of the mould can be influenced by factors such as different regions, raw materials, production processes and the like. The method enriches the microorganism resource library, increases excellent mould resources available for enterprises to select, and has important significance for solving the problems. Disclosure of Invention The invention aims to provide aspergillus oryzae with multiple tolerability, and a separation method and application thereof, so as to solve the problems of insufficient saccharification force and adaptability of aspergillus oryzae in the prior art. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: aspergillus oryzae (Aspergillus oryzae) with multiple tolerance is strain A. Oryzae TH004, and is preserved in China general microbiological culture collection center (CGMCC) No.40448 at 2022 and 12 months and 25 days. The strain a. Oryzae TH004 grew well at 25-45 ℃. The strain A. Oryzae TH004 grows well at a pH of 2-7. The strain A. Oryzae TH004 grows well under the condition that the ethanol content is 0-6%vol. The culture conditions of the bran koji produced by inoculating the strain A. Oryzae TH004 were optimized by the response surface method. Further, the response surface method optimization determines that the saccharification power of the bran koji inoculated by the strain A.oryzae TH004 is obviously improved under the conditions of 28 ℃ of temperature, 49.54% of water content and 6.62% of inoculation amount. A method for isolating aspergillus oryzae having multiple tolerability comprising the steps of: step 1, separating and purifying mould from high saccharification force Daqu; And 2, detecting the saccharification force of the mould obtained in the step 1, and screening to obtain the aspergillus oryzae strain with high saccharification force based on a detection result. And 3, detecting the tolerance of the aspergillus oryzae obtained in the step 2, and screening the aspergillus oryzae strain with multiple tolerance based on a detection result. An application of Aspergillus oryzae with multiple tolerance in wine production is provided to improve saccharification force of wine raw material. A method for improving saccharification power of distiller's yeast comprises adding Aspergillus oryzae with multiple tolerance into distiller's yeast raw material to improve saccharification power of distiller's yeast. Compared with the prior art, the invention has the advantages that: The invention provides aspergillus oryzae with multiple excellent characteristics of high temperature resistance, acid resistance, ethanol resistance, high saccharification force and the like, a separation method thereof and a bran koji culture condition optimization method. The invention has important value in the aspects of saving grains, improving efficiency, saving energy, reducing emission and the like. Drawings FIG. 1 is a graph comparing mold saccharification force. Wherein, the lower case letters are different to show that the difference is obvious (P < 0.05), and the numerical values in the graph are repeated for more than 3 times. FIG. 2 is an agarose gel electrophoresis of PCR amplified ITS1-5.8S-ITS 2. Wherein, negative control, A, TH001, B, TH002, C, TH003, D, TH004, E, TH005, F, TH006