CN-116807964-B - Hydrogel spray liquid for preventing traumatic ectopic ossification, preparation method and spraying device

Abstract

The invention discloses a hydrogel spray liquid for preventing traumatic ectopic ossification, a preparation method and a spraying device, wherein the hydrogel spray liquid comprises photo-curing hydrogel and nano drug-carrying complex loaded with curcumin, and the nano drug-carrying complex is uniformly dispersed in the photo-curing hydrogel to form hydrogel spray; the preparation method comprises the process of ultrasonically dispersing the nano medicine-carrying complex in the photo-curing hydrogel, wherein the spraying device comprises a liquid spraying container for containing hydrogel liquid spraying and a spraying head. The spray has strong penetrating power, is convenient to operate, does not stimulate the intestines and stomach of a human body, and can be used for long-acting prevention of traumatic ectopic ossification.

Inventors

• ZHU WANBO
• YANG JIAZHAO
• XU DONGDONG
• FANG SHIYUAN
• XU LEI
• ZHANG XIANZUO
• ZHU CHEN
• LU BAOLIANG

Assignees

• 安徽省立医院(中国科学技术大学附属第一医院)

Dates

Publication Date

20260508

Application Date

20230328

Claims (6)

1. 1. The hydrogel spray for preventing traumatic ectopic ossification is characterized by comprising a photo-cured hydrogel and a nano drug-loaded complex loaded with curcumin, wherein the nano drug-loaded complex comprises zinc-based zeolite imidazole ester skeleton nano particles used as a drug-loaded bracket for encapsulating the curcumin and nano cerium oxide used as a gating material, and the nano drug-loaded complex is uniformly dispersed in the photo-cured hydrogel to form the hydrogel spray; The photo-curing hydrogel is methacrylic acylated hyaluronic acid hydrogel added with a photoinitiator.
2. 2. A method of preparing a hydrogel spray as recited in claim 1, comprising the steps of: Step 1, preparing a nano drug-carrying complex; And step 2, adding the obtained nano medicine carrying complex into photo-curing hydrogel to carry out ultrasonic dispersion, so as to obtain the hydrogel spray liquid.
3. 3. The preparation method of the hydrogel spraying liquid according to claim 2 is characterized by comprising the following steps of 1.1, co-dissolving 2-methylimidazole and curcumin in methanol to form a mixed solution, dropwise adding a zinc nitrate hexahydrate solution into the mixed solution, stirring and reacting for 13-17 minutes to obtain an opacified suspension, 1.2, dispersing nano cerium oxide particles and polyvinylpyrrolidone in methanol, adding the methanol containing the nano cerium oxide particles and polyvinylpyrrolidone into the opacified suspension, stirring and reacting for 4-6 minutes at room temperature, centrifuging after the stirring reaction is finished, removing impurities to obtain a reaction end product, and 1.3, washing and drying the obtained reaction end product to obtain nano cerium oxide gate zinc-based zeolite imidazole ester skeleton nano particles loaded with curcumin, namely the nano drug-carrying complex.
4. 4. The method for preparing a hydrogel spray according to claim 3, wherein in step 1.2, after the stirring reaction, the reaction product is obtained by centrifugation for 8-11 minutes, washing with methanol to remove impurities, and centrifugation for 28-32 minutes.
5. 5. The method for preparing the hydrogel spray according to claim 2, wherein in step 2, the nano drug-loaded complex is added into the photo-curing hydrogel, and then the hydrogel spray is obtained by ultrasonic dispersion at room temperature for 14-17 minutes.
6. 6. A spray device comprising a spray container and a spray press head mounted to the spray container, the spray container containing a spray, wherein the spray is the hydrogel spray of claim 1.

Description

Hydrogel spray liquid for preventing traumatic ectopic ossification, preparation method and spraying device Technical Field The invention relates to the field of traumatic ectopic ossification prevention medicines, in particular to hydrogel spray for preventing traumatic ectopic ossification, a preparation method and a spraying device. Background Traumatic ectopic ossification is the most common acquired ectopic ossification, which is defined as the pathological process by which tissue damage caused by a wound stimulates ectopic production of bone in the soft tissue surrounding the wound. Traumatic ectopic ossification usually occurs around the large joints of the human body such as elbows, hips, and the like, so that the joint pain and swelling, the movement limitation, the joint rigidity and other functions loss are caused, and the disability is caused. Traumatic ectopic ossification is currently thought to be due to the association of osteogenic signals activated by inflammatory microenvironment cascades and transduction pathways at the time of trauma, and thus control of local tissue inflammatory responses at an early stage helps prevent traumatic ectopic ossification. However, traumatic ectopic ossification is characterized by uncertainty in whether each wound is induced, in the specific location of the onset, in the specific time of onset, and in the specific scope of onset. Once the disease is developed, the disease can be removed only through surgery, and the disease is difficult to be thoroughly removed for a wider range of focuses, thus bringing a heavy burden to patients and society. The existing means for preventing traumatic ectopic ossification are oral non-steroidal anti-inflammatory drugs and prophylactic radiation therapy. The oral non-steroidal anti-inflammatory drug has simple operation, but limited effect, limited penetration of oral drug to local tissues, obvious side effects such as gastrointestinal irritation and the like, and can not maintain the preventive effect for a long time. The equipment required for preventive radiotherapy is expensive and cannot be conventionally developed. It can be said that no ideal preventive mode exists at present, and the surgical injury can be locally intervened in the operation, and a long-acting preventive effect is obtained. Disclosure of Invention The invention provides hydrogel spray for preventing traumatic ectopic ossification, a preparation method and a spraying device thereof, and aims to solve the problem that the traumatic ectopic ossification is difficult to prevent for a long time. In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: The hydrogel spray liquid for preventing traumatic ectopic ossification comprises a photo-cured hydrogel and a nano drug-loaded complex loaded with curcumin, wherein the nano drug-loaded complex comprises zinc-based zeolite imidazole ester skeleton nano particles used as a drug-loaded bracket for encapsulating the curcumin and nano cerium oxide used as a gating material, and the nano drug-loaded complex is uniformly dispersed in the photo-cured hydrogel to form the hydrogel spray liquid. Further, the photo-curing hydrogel is a methacryloylated hyaluronic acid hydrogel added with a photoinitiator. The preparation method of the hydrogel spray liquid comprises the following steps: Step 1, preparing a nano drug-carrying complex; And step 2, adding the obtained nano medicine carrying complex into photo-curing hydrogel to carry out ultrasonic dispersion, so as to obtain the hydrogel spray liquid. Further, the procedure of step 1 is as follows: Step 1.1, 2-methylimidazole and curcumin are dissolved in methanol to form a mixed solution, a zinc nitrate hexahydrate solution is added into the mixed solution in a dropwise manner, and the mixed solution is stirred and reacts for 13-17 minutes to obtain an emulsion suspension; Step 1.2, dispersing nano cerium oxide particles and polyvinylpyrrolidone in methanol, adding the methanol containing the nano cerium oxide particles and polyvinylpyrrolidone into the emulsion suspension, stirring at room temperature for reaction for 4-6 minutes, centrifuging after the stirring reaction is finished, and removing impurities to obtain a reaction end product; And 1.3, washing and drying the obtained reaction end product to obtain the nano cerium oxide-gated zinc-based zeolite imidazole ester skeleton nano-particles carrying curcumin, namely the nano drug-carrying complex. In the further step 1.2, after the stirring reaction is finished, the reaction product is obtained by centrifuging for 8-11 minutes, then washing with methanol to remove impurities, and finally centrifuging for 28-32 minutes. In the further step 2, the nano drug-carrying complex is added into the photo-curing hydrogel, and then the hydrogel is subjected to ultrasonic dispersion for 14-17 minutes at room temperature, so as to obtain the hydrogel spray liquid. A spraying dev