CN-116814455-B - Rhodococcus pyridine and application thereof in degrading nitrocellulose

Abstract

The invention discloses rhodococcus pyridine and application thereof in degrading nitrocellulose. The rhodococcus pyridine is LZ1 and has the preservation number of CCTCC NO: M2022785. The rhodococcus pyridine can convert the nitric oxide removed by the nitrocellulose into the nutrient substances of the rhodococcus pyridine under the condition of ensuring that the skeleton of the nitrocellulose is not damaged, so that the denitration of the nitrocellulose is realized, the nitrite nitrogen and the nitrate nitrogen can be denitrified, the green denitrification of the nitrocellulose is realized, and the water pollution in the treatment process is avoided.

Inventors

• DING YAJUN
• HUANG JUAN
• XUE HUIHUI
• XIAO ZHONGLIANG

Assignees

• 南京理工大学

Dates

Publication Date

20260512

Application Date

20220602

Claims (5)

1. 1. Rhodococcus pyridine (Rhodococcuspyridinivorans) is characterized by being Rhodococcus pyridine LZ1, and the preservation number is CCTCC NO: M2022785.
2. 2. The method for culturing rhodococcus pyridine according to claim 1, comprising the steps of: Rhodococcus pyridine is inoculated into LB liquid medium and dynamically cultured at 37 ℃.
3. 3. The culture method according to claim 2, wherein the LB liquid medium has a formula of 5g/L glucose, 5g/L yeast powder, 10g/L peptone and a natural pH.
4. 4. Use of rhodococcus pyridine for degrading nitrocellulose according to claim 1.
5. 5. The method according to claim 4, wherein the degradation of the nitrocellulose is carried out by inoculating Rhodococcus pyridine to the wastewater containing nitrocellulose.

Description

Rhodococcus pyridine and application thereof in degrading nitrocellulose Technical Field The invention belongs to the field of microorganism treatment of wastewater, and relates to rhodococcus pyridine and application thereof in degrading nitrocellulose. Background The propellant acts as a material control factor for the effective implementation of modern barrel weapons and is the support and foundation for advanced barrel systems. Where nitrocellulose is the main component of the propellant, there is a large number of military nitrocellulose scrapped each year. Military nitrocellulose is an explosive material, and any waste produced during its production is classified as K044 hazardous waste. Therefore, it is of great importance to find a safe and pollution-free method for degrading nitrocellulose. The biological method for treating the nitrocellulose is a novel pollution-free treatment technology, has the advantages of safety, low cost, greenness and the like, and meets the requirements of ecological environment protection under the condition of rapid development of global economy. Early researchers used composting to treat nitrocellulose with reduced nitrogen, which degraded organics by the combined action of heat and thermobacteria, and although the composting process was simpler, the composting time was longer and the degree of nitrocellulose reduced nitrogen was limited. The single strain is adopted to carry out the nitrogen reduction treatment of the nitrocellulose, so that the nitrogen reduction time of the nitrocellulose can be shortened, the denitrification process can be controlled easily, the later industrial biological treatment of the nitrocellulose is facilitated, the cost can be reduced, and the economic and green integrated treatment is achieved. Chinese patent application CN202110080661.X discloses a sulfate reducing bacterium which can utilize nitrate and nitrite degraded on nitrocellulose as self nutrient substances, but the strain needs strict anaerobic environment, has complex operation process and higher economic cost. Chinese patent application CN202110073488.0 discloses a strain of chaetomium macrostemon which can also be used for denitrating nitrocellulose, but the chaetomium macrostemon belongs to fungi, has long growth period and is not beneficial to industrialized treatment of nitrocellulose. Meanwhile, the sulfate reducing bacteria and Gao Damao shell bacteria have lower nitrogen reduction degree on the nitrocellulose, and can not fully convert nitrate nitrogen in the solution in the denitration process, so that the environment-friendly denitration of the nitrocellulose can not be realized. Chinese patent application CN201110006790.0 discloses a strain of rhodococcus pyridinphilia which, although able to effectively utilize nitrate nitrogen and ammonia nitrogen compounds in solution and convert them to a maximum extent into pollution-free gas N 2, is unable to denitrate nitrocellulose. Disclosure of Invention The invention provides rhodococcus dipyridis (Rhodococcus pyridinivorans) LZ1 with a nitrocellulose degrading effect. The rhodococcus pyridine is capable of denitrifying nitrocellulose under aerobic conditions and converting nitrate and nitrite into self-nutrient substances. The preservation number of the rhodococcus pyridine is CCTCC NO: M2022785, the preservation time is 2022, 6 and 2 days, and the preservation address is university of Wuhan in Wuhan, china. The invention also provides a culture method of the rhodococcus pyridine, which comprises the following steps: Rhodococcus pyridine is inoculated into LB liquid medium and dynamically cultured at 37 ℃. The LB liquid culture medium is conventionally used in the field, and has the formula of 5g/L glucose, 5g/L yeast powder, 10g/L peptone and natural pH. Furthermore, the invention also provides application of the rhodococcus pyridine-philic in degrading nitrocellulose. The specific method for the application is that rhodococcus pyridine is inoculated into the wastewater containing the nitrocellulose to degrade the nitrocellulose. Compared with the prior art, the invention has the following advantages: (1) The rhodococcus pyridine-philic can denitrify the nitrocellulose under the aerobic condition, does not need a strict anaerobic environment, and effectively reduces the nitrogen content of the nitrocellulose; meanwhile, the rhodococcus pyridine can utilize nitrate nitrogen and nitrite nitrogen under the denitrification of the nitrocellulose as the nutrition matters for self growth and reproduction; (2) The rhodococcus pyridine-philic has no harmful nitrate and nitrite generated in the denitration treatment of the nitrocellulose, and no secondary pollution to water quality; (3) The rhodococcus pyridine-philic culture medium has the advantages of simple components, easy acquisition of raw materials, simple components, simple and convenient denitration reaction process, and suitability for industrial application, and is a