CN-116814478-B - Chromata slow rooting and application thereof

Abstract

The invention discloses a rhizobium chrobium and application thereof, and belongs to the technical field of nitrogen fixation symbiotic microorganisms. The slow rooting tumor bacteria are preserved in China general microbiological culture collection center (CGMCC) No.27064. The strain has good growth promoting property, and has strong nitrogen resistance and broad spectrum. Under the condition of reducing the application amount of nitrogen fertilizer, the strain can obviously increase the root nodule number, the side branch number, the main stem length and the fruit needle number of peanuts in the full bloom stage and improve the yield of the peanuts in the mature stage, thereby providing a strain source for obtaining rhizobium inoculants suitable for planting various varieties of peanuts, providing a technical basis for reducing the application of nitrogen fertilizer and saving the planting cost, providing high-efficiency stable growth-promoting microbial germplasm resources, expanding the symbiotic bacterial germplasm resources of oil crops in China and providing a basis for developing high-efficiency stable microbial fertilizers and application technologies.

Inventors

• WU YUE
• YANG JISHUN
• WU ZHENGFENG
• ZHANG ZHIMENG
• ZHENG YONGMEI
• YU TIANYI
• WU JUXIANG
• LI SHANGXIA

Assignees

• 山东省花生研究所

Dates

Publication Date

20260512

Application Date

20230608

Claims (8)

1. 1. A Bradyrhizobium sp, wherein the strain is deposited in the China general microbiological culture collection center with a deposit number of CGMCC No. 27064.
2. 2. Use of the slow rooting tumor bacteria according to claim 1 for peanut growth promotion and/or peanut nitrogen saving and/or peanut yield improvement.
3. 3. A rhizobia agent comprising the bradyrhizobium sp according to claim 1.
4. 4. A rhizobial inoculant according to claim 3, wherein the inoculant is in a form selected from the group consisting of a liquid inoculant, a powder or a granule.
5. 5. The rhizobial inoculant of claim 4, wherein the concentration of slow-yielding rhizobia in the liquid inoculant is 1 x 10 10 ~ 1×10 11 cfu/mL.
6. 6. Use of a bradyrhizobium of claim 1 or a rhizobium inoculant of claim 3 in the preparation of a microbial fertilizer.
7. 7. A microbial fertilizer comprising the bradyrhizobium of claim 1 or the rhizobium inoculant of claim 3.
8. 8. The rhizobium seed coating agent is characterized by comprising a slow rooting rhizobium bacterial liquid, a microelement mother solution and a sodium carboxymethyl cellulose solution; The slow rooting rhizobium bacterial liquid is obtained by culturing the slow rooting rhizobium of claim 1 by adopting a TY liquid culture medium, wherein the concentration of the slow rooting rhizobium in the bacterial liquid is 1 multiplied by 10 10 ~ 1×10 11 cfu/mL; The trace element mother solution comprises ：H 3 BO 3 2.86g/L,MnSO 4 1.81g/L,CuSO 4 ·5H 2 O 0.80g/L,ZnSO 4 0.22g/L,H 2 MoO 4 0.02g/L, parts of water; The sodium carboxymethyl cellulose solution is selected from 1% sodium carboxymethyl cellulose aqueous solution, and the preparation method comprises dissolving 10g sodium carboxymethyl cellulose in 1L deionized water at 60 ℃, and stirring to obtain transparent pasty glue solution.

Description

Chromata slow rooting and application thereof Technical Field The invention belongs to the technical field of nitrogen fixation symbiotic microorganisms, and particularly relates to a slow rooting tumor bacterium and application thereof. Background Chromorhizobium is a class of gram-negative bacteria that is widely distributed in soil. Peanut can form root nodule through symbiotic with slow rooting tumor bacteria, and fix nitrogen in air into ammonia capable of being absorbed and utilized by plant. According to statistics, the root nodule can provide 60-65% of nitrogen for peanuts, can partially replace chemical nitrogenous fertilizer to meet the demand of peanut fertility on nitrogen, has huge nitrogen-saving potential, and can reduce the pollution of chemical fertilizer to the environment. Calculated by 490 ten thousand hectares of planting area, 71 ten thousand tons of urea can be saved in one growing season, 14.2 hundred million yuan of expenditure is saved, and 106.5 ten thousand tons of coal is saved. Therefore, the symbiosis of the slow rooting tumor bacteria and the peanuts has great potential in reducing the application amount of nitrogenous fertilizer, protecting the environment and saving energy. The nitrogen content in the soil has a great influence on the nitrogen fixation efficiency of the root nodule, the nitrogen fixation effect of the root nodule can be obviously inhibited when the nitrogen concentration is too high, and the inhibition degree is positively correlated with the fertilization amount and the application time. Excess nitrogen in the soil reduces nodule formation by inhibiting attachment and infestation of plant roots by rhizobia, by activating nitrogen feedback regulation, hormone feedback regulation and nodulation automatic regulation systems in the plant. When symbiotic relationships are difficult to establish, plant growth and yield will become more dependent on nitrogen fertilizer, causing further damage to the environment by nitrogen fertilizer and further difficulty in improving crop yield and quality. The nitrogen-resistant rhizobia can break the situation, the rhizobia can carry out nodulation symbiosis with leguminous crops in high nitrogen fertilizer soil, nitrogen sources are provided for the plants through nitrogen fixation, so that the dependence and the demand of the crops on nitrogen fertilizer are reduced, the high yield of the crops is ensured under the condition of not applying nitrogen fertilizer or reducing the application amount of the nitrogen fertilizer, and the quality of the crops is improved. Although the rhizobia agent has a plurality of advantages in agricultural production, the effect of the rhizobia agent in production is extremely unstable because each rhizobia agent has a peanut variety which is preferred. Therefore, in the current situation, it is difficult to popularize the rhizobium japonicum and expand the application range of the rhizobium japonicum. The key problems faced at the present stage are how to screen and obtain peanut rhizobia strains with strong nitrogen resistance, wide application range, nitrogen fertilizer saving and high peanut yield promotion so as to further prepare rhizobia bacterial agents suitable for peanut planting. Disclosure of Invention The invention provides a Bradyrhizobium sp, which is preserved in China general microbiological culture Collection center with the preservation number of CGMCC No. 27064. The slow-producing rhizobia has better nitrogen resistance, symbiotic broad spectrum, growth promoting function and nitrogen saving and high capacity. Based on the above, the invention provides the application of the slow-growing rhizobia in plant growth promotion and/or nitrogen-saving and high yield promotion. The invention provides a rhizobia agent, which contains the slow-growing rhizobia. In addition, the microbial inoculum can also contain other conventional auxiliary materials. The rhizobia agent can be selected from liquid, powder or granule. In a specific embodiment, the microbial inoculum is specifically a liquid microbial inoculum, the liquid microbial inoculum is obtained by culturing the slow-growing rhizobia in TY liquid culture medium, and the concentration of the slow-growing rhizobia in the liquid microbial inoculum is 1 multiplied by 10 10 ~ 1×1011 cfu/mL. The invention provides application of the rhizobia agent in plant growth promotion and/or nitrogen-saving high yield promotion. The slow-growing rhizobium or rhizobium inoculant can replace part of nitrogenous fertilizer, and the slow-growing rhizobium or rhizobium inoculant, phosphate fertilizer and potash fertilizer form microbial fertilizer. Based on the above, the invention provides the application of the slow-growing rhizobia or rhizobia agent in preparing microbial fertilizer. The invention provides a microbial fertilizer, which contains the slow-growing rhizobium or rhizobium inoculant. The microbial fertilizer can also contain phosphate fe