CN-116837088-B - SNP molecular marker located in IFITM1 gene and related to pig interstitial diseases, primer and application

Abstract

The invention relates to the technical field of pig selective breeding, in particular to SNP molecular markers, primers and application of an IFITM1 gene and a pig intervenereal disease. The nucleotide sequence of the molecular marker is SEQ ID NO.1, the 32 nd base Y in the nucleotide sequence of the SNP molecular marker is a polymorphic site, individuals with genotypes TT and TC at the polymorphic site show interstitial disease characteristics, individuals with genotypes CC have NO interstitial disease characteristics, and the SNP molecular marker is positioned in the 2 nd exon in the IFITM1 gene of the chromosome 2 of the pig. The SNP molecular marker is closely related to the characteristics of the pig intervenereal disease, and the primer disclosed by the invention is used for detecting the SNP locus, screening individuals carrying pathogenic genes, and preventing the transmission of the pig intervenereal disease.

Inventors

• LI HUA
• YU CONGYING
• WU JINHUA
• Zhong Bingzhou
• ZHAO HAIQUAN
• YU HAIYI
• PEI YANGLI

Assignees

• 佛山科学技术学院

Dates

Publication Date

20260505

Application Date

20230625

Claims (2)

1. 1. A primer of SNP molecular marker related to pig intervenereal disease located in IFITM1 gene is used for breeding pig against pig intervenereal disease, which is characterized in that the nucleotide sequence of the molecular marker is SEQ ID NO. 1, the 32 th base Y in the nucleotide sequence of the SNP molecular marker is polymorphic site, at the polymorphic site, individuals with genotype TT and TC show intervenereal disease characteristics, and individuals with genotype CC show intervenereal disease characteristics; the SNP molecular marker is positioned in the 2 nd exon in the IFITM1 gene of the chromosome 2 of the pig; the SNP molecular marker is used for screening individuals with genotype of CC of the SNP molecular marker as breeding parents in breeding of breeding pigs.
2. 2. A primer of SNP molecular markers related to the pig interstitial diseases and located in the IFITM1 gene is used for breeding the pig resistant to the pig interstitial diseases, and is characterized in that the primer is used for amplifying the SNP molecular markers related to the pig interstitial diseases and located in the IFITM1 gene, and comprises an upstream primer and a downstream primer: the upstream primer F1: 5'-TCACCCTTGGGACTCAGACC-3', The downstream primer R1: 5'-AGTGTGGATGGTGGGGGAC-3'.

Description

SNP molecular marker located in IFITM1 gene and related to pig interstitial diseases, primer and application Technical Field The invention relates to the technical field of pig selective breeding, in particular to SNP molecular markers, primers and application of the SNP molecular markers, the primers and the application of the SNP molecular markers are located in IFITM1 genes and are related to pig intervenereal diseases. Background In the introduction of pigs or the promotion of semen, it is often required that the exporter mark in the pedigree or write that the breeding pigs do not have genetic defects in the terms of sales contracts. However, the carrier suffering from the pig genetic defect has no expression character, so heterozygotes cannot be effectively eradicated or the cause of the genetic defect cannot be judged, and a certain bottleneck appears in the breeding work. In addition, the pig-to-pig disease is a genetic defect disease which seriously affects the quality of the source, and mostly occurs in the introduced long white pigs, large white pigs and hybrid commercial pigs thereof, which is a technical breeding bottleneck problem faced by pig raising industry in China and even the world. In addition, the similarity of pigs to humans in terms of anatomical size and structure, physiology, immunology, etc. enhances their potential as human models. Thus, the pig-to-pig disease can also provide reference value for related diseases of human beings. Sex dysplasia (Disorder of sex development, DSD) is a disease caused by congenital genetic factors, resulting in a difference in the phenotype of the external genitalia and the inherited chromosomes of individuals, and in most species of pigs suffering from an interstitial disease, it has a normal female chromosome structure (38, xx) and is free of male sex determining genes (Sex Determining Region Y, SRY-), whose gonads develop into the egg testosterone (Ovotestis, OT) or immature testes (Testis, T), which is called pig 38, xx-OT/T-DSD (SRY-). XX-OT/T-DSD (SRY-) belongs to an autosomal genetic disease, the incidence rate of which is about 0.1-0.6% in different pig herds, and up to 20% in some independent inbred populations. The intermittent pigs not only can cause infertility, but also can cause growth and development and survival rate to be reduced, and the grade of carcasses to be reduced due to the smell of the boars, and the intermittent pigs also can show a certain delicacy, so that the production of high-quality breeding pigs in pig raising production industry in China and even in the world is severely restricted. At present, the pig breeding mode in China mainly adopts intensive breeding, and a large number of artificial fertilization technology is adopted for breeding and protecting the breeding pigs, so that the risk of incidence of the interstitial pigs is increased. If the molecular screening can be carried out on the artificial inseminated boar or the semen thereof, the interjacent diseases are prevented from being amplified and transmitted by the artificial insemination 1 (female): 80-200 (female): can achieve the aim of assisting in eliminating the harm of the interjacent pigs, and the guarantee is provided for the high-quality boar in China. The economic benefit of the pig farm can be effectively improved by preventing the intermittent diseases. At present, the genetic mode and molecular etiology of the pig are not quite clear, and the discussion of the causes of the pig's intervenereal disease is of great significance in reducing the incidence rate of the disease. The invention uses high-throughput sequencing technology and molecular biology means to identify pathogenic genes of the swine intermediate diseases, and development of molecular markers is helpful for preventing the occurrence of the swine intermediate diseases. As a single nucleotide polymorphism (Single nucleotide polymorphism, SNP) of the third generation molecular marker technology, the method has the characteristics of co-dominance, automatic detection, rich genetic diversity and the like, and is widely applied to the typing and identification of medical and animal diseases. Currently, in mammalian XX-DSD studies, a number of XX-DSD related SNPs have been reported, such as the homozygous mutation c.332G > A (p.cys11 tyr) in the furin-like cysteine-rich domain-2 (furin-LIKE CYSTEINE-rich domain-2, FU-CRD 2) of the human R-spinal protein 1 (R-spondin 1, rspo 1) gene, and the homozygous mutation c.43_43delA (p.Thr15Argfs. Times.77) of the RSPO1 gene are all related to 46, XX-DSD. The missense mutation of the heterozygous for human Wnt family member 4 (WNT FAMILY membrane 4 gene, wnt 4) can lead to a different degree of maleation in 46, xx women, including androgen excess and muller dysplasia, while the homozygous mutation of Wnt4 can cause 46, xx-DSD. It was found that heterozygous mutation of the nuclear receptor subfamily 5 group A member 1 (nuclear receptor subfamily