CN-116850352-B - Double-layer artificial periosteum for guided bone tissue regeneration and preparation method thereof

CN116850352BCN 116850352 BCN116850352 BCN 116850352BCN-116850352-B

Abstract

The invention belongs to the technical field of artificial bionic materials, and particularly relates to a double-layer artificial periosteum for guiding bone tissue regeneration and a preparation method thereof. The double-layer artificial periosteum is formed by compounding a compact layer and a loose layer, wherein the compact layer is prepared by photo-crosslinking a PBS buffer solution containing raw materials with the mass volume fraction of 60-90% of methacryloyl gelatin and 0.1-10% of photoinitiator, and the loose layer is prepared by photo-crosslinking a PBS buffer solution containing raw materials with the mass volume fraction of 60-90% of methacryloyl gelatin, 0.1-10% of photoinitiator and 5-20mg/ml of nano-scale hydroxyapatite. The invention obtains the double-layer artificial periosteum with excellent repairing effect on bone defect by optimizing the material composition and the preparation process of the double-layer artificial periosteum, and simultaneously has good swelling coefficient, mechanical strength and degradation rate, thereby having good application prospect.

Inventors

ZHANG WENJIE
TIAN YE
WANG JIANGYUE
BAI DING

Assignees

四川大学华西医院
四川大学

Dates

Publication Date: 20260505
Application Date: 20230531

Claims (2)

1. A double-layer artificial periosteum for guiding bone tissue regeneration is characterized by being formed by compounding a compact layer and a loose layer; Wherein, the compact layer is prepared by photo-crosslinking a PBS buffer solution containing the following raw materials by mass and volume fraction: 10% of methacryloyl gelatin with a grafting degree of 90%, 0.5% Of a photoinitiator; the time for photocrosslinking the compact layer is 30s; The loose layer is prepared by photo-crosslinking a PBS buffer solution containing the following raw materials in percentage by mass and volume: 10% of methacryloyl gelatin with grafting degree of 60%, 0.5% Of photoinitiator, 10 Mg/ml of nano-scale hydroxyapatite; The time for photocrosslinking the loose layer was 10s.
2. The method for preparing a double-layered artificial periosteum for guided bone tissue regeneration according to claim 1, comprising the steps of: Step 1, adding a raw material for preparing a compact layer and PBS buffer solution into a die, and carrying out photocrosslinking; step2, adding the raw materials for preparing the loose layer and PBS buffer solution into a die with a formed compact layer, and carrying out photo-crosslinking; and 3, carrying out vacuum freeze-drying to obtain the product.

Description

Double-layer artificial periosteum for guided bone tissue regeneration and preparation method thereof Technical Field The invention belongs to the technical field of artificial bionic materials, and particularly relates to a double-layer artificial periosteum for guiding bone tissue regeneration and a preparation method thereof. Background Bone tissue defects caused by trauma, tumor, inflammation, dysplasia and the like are common clinical problems, and endanger the health and the quality of life of patients. Bone defects in the oromaxillofacial region often cause loosening or even loss of teeth and limit development of related treatments such as implant restoration and orthodontic techniques. Repair and reconstruction of bone defects is a great challenge to orthopedics and stomatologists from time to time. Guided bone tissue regeneration (Guided bone regeneration, GBR) is a currently in clinical use method for repairing bone defects, which is based on the principle that a barrier membrane is used to isolate the growth of epithelial cells and fibroblasts which grow faster into a bone defect area so as to prevent the cells from interfering with bone repair, construct and maintain the growth space of osteoblast-related cells and blood vessels, protect blood clots and other bone repair materials in the defect, promote the growth, proliferation, differentiation and migration of osteoblast-related cells, and further accelerate the formation of new bone. In GBR technology, barrier membranes play an important role, the performance of which determines to some extent the success or failure of GBR. The ideal barrier membrane should possess good biocompatibility, cell barrier effect, suitable physicochemical and mechanical properties, biodegradability, promotion of osteogenic effects, etc. GBR membranes commonly used in clinic can be divided into two categories, non-absorbable membranes and absorbable membranes. The non-absorbable membrane often needs to be removed by a secondary operation, and the removal time is not easy to control, so that the treatment effect is easily affected. The traditional absorbable membrane comprises natural collagen, chitosan, synthetic polyester materials and the like, has good biocompatibility and biodegradability, but has poor physical properties, insufficient mechanical properties, high degradation speed, high swelling ratio and the like, and is easy to enable fibroblast cells which grow faster to invade a bone defect area, further prevent the repair of bone tissues and difficult to completely meet clinical requirements. The GBR membrane widely used in clinic at present is mainly developed by Swiss Gauss companyThe absorbable barrier film has a double-layer film structure with different degrees of density, and comprises pigskin type I collagen and type II collagen which are not subjected to crosslinking treatment. As a pure collagen film, the collagen film,The method also has the defects of higher swelling ratio, too high degradation speed, poor mechanical properties and the like, and needs to be further improved and optimized. The properties of the collagen material can be improved by chemical crosslinking, but chemical crosslinking agents such as glutaraldehyde and the like have problems of cytotoxicity or being unfavorable for bone tissue regeneration and the like. Methacrylic Anhydride (MA) can undergo amidation reaction with active amino groups in materials such as collagen, gelatin, chitosan and the like, so that the materials have a methacryloyl group, and methacryloyl gelatin (gelatin methacryloyl, gelMA) is formed. The material can be endowed with the capability of cross-linking and curing under the irradiation of visible light or ultraviolet light after different types of photoinitiators are added. The photocrosslinking process is simple and convenient, the requirement on conditions is not high, the photocrosslinking process is only carried out at room temperature, in a neutral pH value range and under the condition of aqueous solution, and the crosslinking degree can be controlled by controlling the illumination range and time. The material not only maintains the biological activity of natural materials, such as cell adhesion promotion, bone activity promotion and the like, but also has high adjustability of physicochemical properties, and can realize the improvement of the heterogeneous double-layer structure of the GBR barrier membrane and the physicochemical properties thereof. Based on the thought, the Chinese patent No. CN114558172B provides a double-layer bionic artificial periosteum, a preparation method and application thereof, and the double-layer bionic artificial periosteum comprises a bionic hair growing layer and a bionic fiber layer, wherein the bionic hair growing layer is made of hydrogel prepared from methacryloylated gelatin and nano hydroxyapatite. The bionic fiber layer is made of hydrogel prepared from methacryloylated gelatin and N-acryl 2-glycine. Alth