CN-116858590-B - Biochemical sample slicing device and slicing method

CN116858590BCN 116858590 BCN116858590 BCN 116858590BCN-116858590-B

Abstract

The invention relates to the field of biochemistry, in particular to a biochemical sample slicing device and a slicing method. The slicing device comprises a round seat, wherein middle frames are fixed on two sides of the round seat, each middle frame is hinged with a rotating rod, and the lower part of each rotating rod is fixed with a fork. The slicing method comprises the steps of S1, clamping a biological sample between two forks, S2, immersing the biological sample in wax through the two forks, S3, driving the two clamping pieces to clamp the biological sample, loosening the two forks, S4, alternately clamping the two forks and the two clamping pieces on the biological sample, wrapping the biological sample with the wax layer by layer, S5, placing the biological sample wrapped with the wax on a disc, and slicing the biological sample layer by moving the biological sample wrapped with the wax tangentially by a cutter left and right. The biological sample may be coated with wax layer by layer and then sectioned layer by layer.

Inventors

SUN ZHE
LV YING
CUI YONGZHEN

Assignees

东北农业大学

Dates

Publication Date: 20260508
Application Date: 20230705

Claims (2)

1. The biochemical sample slicing device comprises a round seat (103) and is characterized in that middle frames (104) are fixed on two sides of the round seat (103), a rotating rod (106) is hinged on each middle frame (104), and a fork (107) is fixed on the lower part of each rotating rod (106); A motor (105) is fixed on each middle frame (104), and the motor (105) drives a corresponding rotating rod (106) to rotate; The round seat (103) is fixed in the middle of the transverse column (101), the upper parts of the two vertical rods (201) are respectively and slidably connected to the left end and the right end of the transverse column (101), and the vertical rods (201) are driven to slide on the transverse column (101) through the first telescopic rod (102); The lower part of the vertical rod (201) is rotatably connected with a rotating shaft (207), clamping pieces (208) are fixed at opposite ends of the two rotating shafts (207), and each clamping piece (208) is provided with a plurality of salient points (209); The outer end of the rotating shaft (207) is fixedly provided with a gear (206), the vertical rod (201) is fixedly provided with a convex seat (204), the convex seat (204) is vertically and slidably connected with a lifting column (202), the lifting column (202) is driven to slide through a telescopic rod II (203), the lower end of the lifting column (202) is fixedly provided with a rack (205), and the rack (205) is meshed with the gear (206) for transmission; the left end and the right end of the transverse column (101) are respectively fixed with a vertical column (108), the two vertical columns (108) are respectively vertically and slidably connected to the two sides of the square box (401), and the vertical columns (108) are driven to slide through a telescopic rod III (405); Round seats (403) are fixed at four corners of the inside of the square box (401), arc-shaped sheets (404) are fixed on the upper sides of the round seats (403), and electric heating wires (402) are fixed on the inner sides of the arc-shaped sheets (404); four L-shaped rods (301) are annularly fixed on the round seat (103), inclined plates (302) are fixed at the lower parts of the four L-shaped rods (301), and the four inclined plates (302) are respectively pressed at the upper parts of four arc-shaped pieces (404); a method of slicing a biochemical sample, comprising the steps of: S1, clamping a biological sample between two forks (107); s2, immersing the biological sample into wax through two forks (107); S3, driving the two clamping pieces (208) to clamp on the biological sample, and loosening the two forks (107); s4, alternately clamping the two forks (107) and the two clamping pieces (208) on the biological sample, and wrapping the wax on the biological sample layer by layer; s5, placing the biological sample wrapped with the wax on a disc (501), and slicing the biological sample layer by moving the biological sample wrapped with the wax tangentially left and right by a cutter (508).
2. A biochemical sample slicing device according to claim 1, wherein a vertical rail (503) is arranged in the middle of the rear side of the square box (401), an L-shaped piece (502) is connected to the vertical rail (503) in a sliding mode, a disc (501) is fixed on the L-shaped piece (502), the L-shaped piece (502) is driven to slide through a telescopic rod four (504), a support (506) is fixed on the rear side of the square box (401), a transverse frame (505) is connected to the support (506) in a sliding mode, a vertical piece (507) is fixed at the left end of the transverse frame (505), a cutter (508) is arranged at the lower portion of the vertical piece (507), and the cutter (508) is located above the disc (501).

Description

Biochemical sample slicing device and slicing method Technical Field The invention relates to the field of biochemistry, in particular to a biochemical sample slicing device and a slicing method. Background Biochemical sample sectioning is a common experimental technique commonly used to study interactions and structures between biomolecules. In this process, it is necessary to cut a sample of biological tissue or cells or the like into very thin sections, which are then stained, microscopically observed or analyzed. In this process, the sample needs to be fixed, and in order to maintain the form and structure of the sample, the sample needs to be fixed by using wax, and then the sample is sliced conveniently, but the prior art cannot wrap the wax on the biological sample layer by layer, and then slice the biological sample layer by layer. Disclosure of Invention In order to overcome the defects of the prior art, the invention provides a biochemical sample slicing device and a slicing method, the method has the beneficial effects that the biological sample can be wrapped by wax layer by layer, and then sliced layer by layer. The utility model provides a biochemical sample section device, includes the circle seat, the both sides of circle seat all are fixed with well frame, all articulate on every well frame has the bull stick, and the lower part of every bull stick all is fixed with the fork. And each middle frame is fixedly provided with a motor, and the motor drives the corresponding rotating rod to rotate. The round seat is fixed in the middle of the cross column, the upper parts of the two vertical rods are respectively connected with the left end and the right end of the cross column in a sliding mode, and the vertical rods slide on the cross column through first driving of the telescopic rods. The lower part of the vertical rod is rotationally connected with a rotating shaft, clamping pieces are fixed at opposite ends of the two rotating shafts, and a plurality of salient points are arranged on each clamping piece. A method of slicing a biochemical sample, comprising the steps of: s1, clamping a biological sample between two forks; s2, immersing the biological sample into wax through two forks; s3, driving the two clamping pieces to clamp on the biological sample, and loosening the two forks; S4, alternately clamping the two forks and the two clamping pieces on the biological sample, and wrapping the wax on the biological sample layer by layer; S5, placing the biological sample wrapped with the wax on a disc, and slicing the biological sample layer by moving the biological sample wrapped with the wax tangentially left and right by a cutter. Drawings The invention will be described in further detail with reference to the accompanying drawings and detailed description. FIG. 1 is a flow chart of a method of slicing biochemical samples; FIG. 2 is a schematic diagram of a biochemical sample slicing device; FIG. 3 is a schematic diagram of a biochemical sample slicing device; FIG. 4 is a schematic diagram of a biochemical sample slice apparatus; FIG. 5 is a schematic view of a cross-pillar structure; FIG. 6 is a second schematic structural view of a cross-post; FIG. 7 is a schematic view of a vertical rod; FIG. 8 is a schematic structural view II of a vertical rod; FIG. 9 is a schematic view of the structure of an L-shaped rod; FIG. 10 is a schematic view of a square box and a disk; Fig. 11 is a schematic diagram of a square box and a disc. In the figure, a cross column 101, a telescopic rod I102, a round seat 103, a middle frame 104, a motor 105, a rotating rod 106, a fork 107 and a vertical column 108; Vertical rod 201, lifting column 202, telescopic rod two 203, boss 204, rack 205, gear 206, rotating shaft 207, clamping piece 208 and salient point 209; an L-shaped rod 301, a sloping plate 302; square box 401, heating wire 402, round seat 403, arc piece 404, expansion link III 405; Disc 501, L-shaped piece 502, vertical rail 503, telescopic rod IV 504, transverse frame 505, bracket 506, vertical sheet 507 and cutter 508. Detailed Description This example can achieve the effect of wrapping wax on the outside of the biological sample, as shown in fig. 5-6. Because biochemical sample section device includes circle seat 103, the both sides of circle seat 103 all weld the well frame 104, all articulate on every well frame 104 has bull stick 106, and the lower part of every bull stick 106 has all welded fork 107, and two bull sticks 106 can rotate on two well frames 104 respectively, and then drive two forks 107 and separate or close, and then can be under the circumstances of reducing the area of contact with biological sample with two forks 107 with biological sample centre gripping, then immerse biological sample in the wax, and then wrap up wax in biological sample's outside. This example may achieve the effect of driving the two prongs 107 apart or closed, as shown in fig. 5-6. Because each middle frame 10