CN-116869901-B - Application of rhizoma paridis extract in inhibiting melanin transfer and cosmetics

Abstract

The invention discloses application of a paris polyphylla extract in inhibiting melanin transfer and a cosmetic, and belongs to the technical field of cosmetics. The paris polyphylla extract comprises beta-ecdysterone, and can play a role in whitening by inhibiting melanin transfer. The cosmetic containing the rhizoma paridis extract has good whitening effect.

Inventors

• WU HUIJUN
• LIU NANNAN
• CAI JIALUN
• ZHANG LIHUA
• ZHU LONG

Assignees

• 欧诗漫生物股份有限公司

Dates

Publication Date

20260508

Application Date

20230825

Claims (4)

1. 1. Use of paris polyphylla extract in the preparation of a product with whitening effect, wherein the paris polyphylla extract comprises beta-ecdysterone; the rhizoma paridis extract is obtained by placing rhizoma paridis raw material purchased from Yunnan western grass in a blast drying oven at 55deg.C, and pulverizing with a traditional Chinese medicine pulverizer to obtain 10 mesh powder; Soaking the powder in 85vt% ethanol-water solution as extraction solvent for 1 hr at a feed-liquid ratio of 1:10, setting extraction temperature of 50deg.C, ultrasonic power of 500W, ultrasonic time of 50min, performing ultrasonic extraction, centrifuging at 5000rpm for 10min, collecting supernatant, and removing impurities by suction; Extracting ethanol extract with n-butanol for 2 times, and mixing the extracts; dividing the extract by using an 80-mesh normal-phase silica gel chromatographic column, and sequentially carrying out gradient elution on the extract by using chloroform-methanol solution according to the volume ratio of chloroform to methanol of 5:1, 4:1, 3:1, 2:1, 1:1 and 0:1, wherein each elution gradient corresponds to the volume of the used eluent to form two beds; Purifying the eluent eluted by the chloroform-methanol volume ratio of 5:1 by adopting a C18 reverse phase silica gel chromatographic column, carrying out gradient elution by using a methanol-water solution according to the methanol-water volume ratio of 10:90, 20:80, 30:70, 40:60 and 50:50 in sequence, wherein the volume of the eluent used by each elution gradient is two beds, taking 200mL of the eluent eluted by the methanol-water volume ratio of 50:50, and evaporating to dryness to obtain paris polyphylla extract powder.
2. 2. The use according to claim 1, wherein the paris polyphylla extract is used for preparing cosmetics having whitening effect.
3. 3. The use according to claim 1 or 2, wherein the paris polyphylla extract is used for inhibiting melanin transfer, wherein inhibiting melanin transfer comprises at least one of the following: a. reducing the number of melanin dendrite structures; b. shortening the length of melanin dendrite; c. reducing melanin content; d. inhibiting melanosome transfer.
4. 4. The use according to claim 1, further comprising concentrating the eluate obtained from the purification after the purification.

Description

Application of rhizoma paridis extract in inhibiting melanin transfer and cosmetics Technical Field The invention relates to the technical field of cosmetics, in particular to application of a paris polyphylla extract in inhibiting melanin transfer and a cosmetic. Background The color of human skin is primarily affected by pigmentation, which not only determines the color of the skin, hair and eyes, but also protects the skin from excessive exposure to ultraviolet light. The process of pigmentation is very complex, involving assembly of melanosomes within the melanocytes and melanin synthesis, transfer of mature melanosomes distally to the dendrites in the direction of cell dendrite extension, and then release of the melanosomes from the melanocytes into surrounding keratinocytes where they redistribute and degrade. The process of transferring melanosomes from the protruding tip of the nucleus Zhou Xiangshu and into the adjacent keratinocytes is commonly referred to as melanosome transport, and studies have shown that melanosome transport plays a very important role in the skin pigmentation process. Paris polyphylla is a plant of Paris genus of Liliaceae, also called Paris polyphylla, etc., and is distributed in Sichuan, yunnan, guangxi, guizhou, jiangxi, hunan and other places in China because its leaves are mostly 7 leaves take place in the stem, and flowers take place on the leaves of the roots of the stems. The paris polyphylla has complex chemical components, and at present, paris polyphylla is mainly used for resisting tumors, resisting inflammation, easing pain, inducing platelet aggregation, hemolysis and the like, and no related technology for inhibiting melanin transfer by using paris polyphylla is seen. In view of this, the present invention has been made. Disclosure of Invention The invention aims to provide an application of paris polyphylla extract in inhibiting melanin transfer. The second object of the present invention is to provide a cosmetic containing the above paris polyphylla extract. The application can be realized as follows: in a first aspect, the present application provides the use of a paris polyphylla extract for inhibiting melanin transfer, wherein the paris polyphylla extract comprises beta-ecdysterone. In an alternative embodiment, the paris polyphylla extract is used for preparing cosmetics with whitening effect. In alternative embodiments, inhibiting melanin transfer comprises at least one of the following: a. reducing the number of melanin dendrite structures; b. shortening the length of melanin dendrite; c. reducing melanin content; d. inhibiting melanosome transfer. In an alternative embodiment, the rhizoma paridis extract is obtained by subjecting rhizoma paridis to ultrasound-assisted alcohol extraction. In alternative embodiments, the ultrasound-assisted alcohol lift includes at least one of the following features: The ultrasonic power is 200-500W; the second characteristic is that the ultrasonic time is 30-60min; the alcohol extraction temperature is 30-60 ℃; the fourth feature is that the alcohol solution used in the alcohol extraction is ethanol-water solution with the ethanol concentration of 60-85vt percent; and fifth, the feed liquid ratio of the rhizoma paridis medicinal material to the alcohol solution is 1:5-1:20. In an alternative embodiment, the alcohol extract obtained by ultrasonic-assisted alcohol extraction is purified, and the chromatographic column used for purification comprises any one of a macroporous resin chromatographic column and a reverse phase silica gel chromatographic column. In an alternative embodiment, the macroporous resin type used for the macroporous resin column comprises at least one of AB-8, D101, HPD-100, and ADS 600; The reverse phase silica gel chromatographic column comprises an RP-18 reverse phase silica gel chromatographic column or a C18 reverse phase silica gel chromatographic column. In an alternative embodiment, the eluent is an ethanol-water solution when purifying with a macroporous resin chromatographic column, and a methanol-water solution when purifying with a reverse phase silica gel chromatographic column. In an alternative embodiment, the purification is performed using a reverse phase silica gel column, followed by a sectioning with a normal phase silica gel column. In an alternative embodiment, the normal phase silica gel column is an 80-100 mesh normal phase silica gel column. In an alternative embodiment, the eluent used in the segmentation process is chloroform-methanol solution. In an alternative embodiment, the method further comprises extracting the ethanol extract and purifying the extract obtained by extraction; And/or, after purification, further comprising concentrating the eluate obtained by purification. In a second aspect, the present application provides a cosmetic comprising a paris polyphylla extract, the paris polyphylla extract comprising beta-ecdysterone. The beneficial effects of the applicat